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Highly Sensitive, Label-Free Detection of 2,4-Dichlorophenoxyacetic Acid Using an Optofluidic Chip
ACS Sensors ( IF 8.2 ) Pub Date : 2017-05-30 00:00:00 , DOI: 10.1021/acssensors.7b00217 Xueling Feng 1 , Gong Zhang 2 , Lip Ket Chin 2 , Ai Qun Liu 2 , Bo Liedberg 1
ACS Sensors ( IF 8.2 ) Pub Date : 2017-05-30 00:00:00 , DOI: 10.1021/acssensors.7b00217 Xueling Feng 1 , Gong Zhang 2 , Lip Ket Chin 2 , Ai Qun Liu 2 , Bo Liedberg 1
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A highly sensitive approach for rapid and label-free detection of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) using an optofluidic chip is demonstrated. The optofluidic chip is prepared by covalent immobilization of 2,4-D-bovine serum albumin (2,4-D-BSA) conjugate to an integrated microring resonator. Subsequent detection of 2,4-D carried out in a competitive immunoreaction format enables selective detection of 2,4-D in different types of water samples, including bottled, tap, and lake water, at a limit of detection (LOD) of 4.5 pg/mL and in a quantitative range of 15–105 pg/mL. The microring resonator-based optofluidic chip is reusable with ultrahigh sensitivity that offers real-time and on-site detection of low-molecular-weight targets for potential applications in food safety and environmental monitoring.
中文翻译：
使用光电芯片对2，4-二氯苯氧基乙酸进行高灵敏度，无标记的检测
演示了使用光流控芯片快速，无标签地检测除草剂2,4-二氯苯氧基乙酸（2,4-D）的高灵敏度方法。通过将2,4-D-牛血清白蛋白（2,4-D-BSA）共轭物共价固定到集成的微环共振器上来制备光流体芯片。随后以竞争性免疫反应形式进行的2,4-D检测，能够以4.5的检出限（LOD）选择性检测包括瓶装水，自来水和湖水在内的不同类型的水样品中的2,4-D。 pg / mL，定量范围为15-10 5pg / mL。基于微环谐振器的光流体芯片具有超高灵敏度，可重复使用，可实时，现场检测低分子量目标，可用于食品安全和环境监测。
更新日期：2017-06-28
中文翻译：
使用光电芯片对2，4-二氯苯氧基乙酸进行高灵敏度，无标记的检测
演示了使用光流控芯片快速，无标签地检测除草剂2,4-二氯苯氧基乙酸（2,4-D）的高灵敏度方法。通过将2,4-D-牛血清白蛋白（2,4-D-BSA）共轭物共价固定到集成的微环共振器上来制备光流体芯片。随后以竞争性免疫反应形式进行的2,4-D检测，能够以4.5的检出限（LOD）选择性检测包括瓶装水，自来水和湖水在内的不同类型的水样品中的2,4-D。 pg / mL，定量范围为15-10 5pg / mL。基于微环谐振器的光流体芯片具有超高灵敏度，可重复使用，可实时，现场检测低分子量目标，可用于食品安全和环境监测。
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