Light-Induced Fluorescence Modulation of Quantum Dot-Crystal Violet Conjugates: Stochastic Off–On–Off Cycles for Multicolor Patterning and Super-Resolution,Journal of the American Chemical Society

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Light-Induced Fluorescence Modulation of Quantum Dot-Crystal Violet Conjugates: Stochastic Off–On–Off Cycles for Multicolor Patterning and Super-Resolution
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2017-05-11 00:00:00 , DOI: 10.1021/jacs.7b02530
Sungwook Jung , Joonhyuck Park , Jiwon Bang ₁ , Jae-Yeol Kim , Cheolhee Kim , Yongmoon Jeon , Seung Hwan Lee , Ho Jin , Sukyung Choi , Bomi Kim , Woo Jin Lee , Chan-Gi Pack ₂ , Jong-Bong Lee , Nam Ki Lee ₃ , Sungjee Kim

Affiliation

Photoswitching or modulation of quantum dots (QDs) can be promising for many fields that include display, memory, and super-resolution imaging. However, such modulations have mostly relied on photomodulations of conjugated molecules in QD vicinity, which typically require high power of high energy photons at UV. We report a visible light-induced facile modulation route for QD-dye conjugates. QD crystal violets conjugates (QD-CVs) were prepared and the crystal violet (CV) molecules on QD quenched the fluorescence efficiently. The fluorescence of QD-CVs showed a single cycle of emission burst as they go through three stages of (i) initially quenched “off” to (ii) photoactivated “on” as the result of chemical change of CVs induced by photoelectrons from QD and (iii) back to photodarkened “off” by radical-associated reactions. Multicolor on-demand photopatterning was demonstrated using QD-CV solid films. QD-CVs were introduced into cells, and excitation with visible light yielded photomodulation from “off” to “on” and “off” by nearly ten fold. Individual photoluminescence dynamics of QD-CVs was investigated using fluorescence correlation spectroscopy and single QD emission analysis, which revealed temporally stochastic photoactivations and photodarkenings. Exploiting the stochastic fluorescence burst of QD-CVs, simultaneous multicolor super-resolution localizations were demonstrated.

中文翻译：

量子点-结晶紫共轭物的光诱导荧光调制：用于多色图案和超分辨率的随机开-关循环

量子点（QD）的光开关或调制对于包括显示，内存和超分辨率成像在内的许多领域都有望实现。但是，此类调制主要依赖于QD附近的共轭分子的光调制，这通常需要在UV处具有高功率的高能光子。我们报告了可见光诱导量子点染料共轭物的简便调制途径。制备了QD结晶紫共轭物（QD-CV），QD上的结晶紫（CV）分子有效地猝灭了荧光。QD-CVs的荧光显示了一个单一的发射脉冲周期，因为它们经历了三个阶段：（i）初始淬灭“关”到（ii）光激活的“开”，这是由于来自QD和（iii）由于自由基相关反应而变暗。使用QD-CV固态胶片演示了多色按需图案化。将QD-CV引入细胞，并用可见光激发产生从“关”到“开”和“关”的光调制近十倍。使用荧光相关光谱和单QD发射分析研究了QD-CV的个体光致发光动力学，揭示了时间上随机的光激活和光暗化。利用随机的QD-CV荧光猝发，证明了同时多色超分辨率定位。使用荧光相关光谱和单QD发射分析研究了QD-CV的个体光致发光动力学，揭示了时间上随机的光激活和光暗化。利用随机的QD-CV荧光猝发，证明了同时多色超分辨率定位。使用荧光相关光谱和单QD发射分析研究了QD-CV的个体光致发光动力学，揭示了时间上随机的光激活和光暗化。利用随机的QD-CV荧光猝发，证明了同时多色超分辨率定位。

更新日期：2017-05-27

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