Based on the recent near-atomic structures of the PYRIN domain of ASC in the protein filament of inflammasomes and the observation that the active form of vitamin B6 (pyridoxal phosphate, P5P) modulates the self-assembly of ASC, we rationally designed an N-terminal capped nonapeptide (Nap-FFKKFKLKL, 1) to form supramolecular nanofibers consisting of α-helix. The addition of P5P to the solution of 1 results in a hydrogel almost instantly (about 4 seconds). Several other endogenous small molecules (for example, pyridoxal, folinic acid, ATP, and AMP) also convert the solution of 1 into a hydrogel. As the demonstration of correlating assemblies of peptides and the relevant protein epitopes, this work illustrates a bioinspired approach to develop supramolecular structures modulated by endogenous small molecules.

中文翻译：

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炎症小体启发的即时水凝胶化

根据炎症小体蛋白丝中ASC的PYRIN域的最新近原子结构，并观察到维生素B6的活性形式（磷酸吡rid醛，P5P）调节ASC的自组装，我们合理地设计了N-末端封端的九肽（Nap-FFKKFKLKL，1）形成由α-螺旋组成的超分子纳米纤维。将P5P添加到1的溶液中几乎可以立即（约4秒）产生水凝胶。其他几个内源性小分子（例如吡ido醛，亚叶酸，ATP和AMP）也可以转化1的溶液。变成水凝胶。作为肽和相关蛋白表位的相关组装的证明，这项工作说明了一种受生物启发的方法来开发由内源性小分子调节的超分子结构。