Structure-Dependent Binding of hnRNPA1 to Telomere RNA,Journal of the American Chemical Society

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Structure-Dependent Binding of hnRNPA1 to Telomere RNA
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2017-05-16 00:00:00 , DOI: 10.1021/jacs.7b01599
Xiao Liu ₁ , Takumi Ishizuka ₁ , Hong-Liang Bao ₁ , Kei Wada ₂ , Yuma Takeda ₁ , Keisuke Iida ₃ , Kazuo Nagasawa ₃ , Danzhou Yang ₄ , Yan Xu ₁

Affiliation

Telomeric repeat-containing RNA is a new noncoding RNA molecule that performs various biofunctions. Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 is an RNA-binding protein involved in the telomere maintenance machinery. To date, little is known about how hnRNPA1 binds to telomeric RNA. In this study, we investigated the binding affinity and recognition mechanism of telomere RNA with the RNA recognition motif of hnRNPA1. Using the photochemical cross-linking method, we showed that the telomere RNA G-quadruplex with loops is important in the interaction of telomere RNA with hnRNPA1. Using small-molecule probes, we directly visualized the complex formed by the telomere RNA G-quadruplex and hnRNPA1 in vitro and in live cells. The results suggested that the structure-dependent binding of hnRNPA1 to telomere RNA regulates the telomere function. Therefore, our study provides new insights into the interactions between the RNA G-quadruplex and proteins at the telomere.

中文翻译：

hnRNPA1与端粒RNA的结构依赖性结合。

含有端粒重复序列的RNA是一种新型的非编码RNA分子，具有多种生物功能。异质核核糖核蛋白（hnRNP）A1是一种涉及端粒维持机制的RNA结合蛋白。迄今为止，人们对hnRNPA1如何结合端粒RNA知之甚少。在这项研究中，我们调查了端粒RNA与hnRNPA1的RNA识别基序的结合亲和力和识别机制。使用光化学交联方法，我们表明带环的端粒RNA G四联体在端粒RNA与hnRNPA1的相互作用中很重要。使用小分子探针，我们可以在体外和活细胞中直接观察端粒RNA G-四链体和hnRNPA1形成的复合体。结果表明，hnRNPA1与端粒RNA的结构依赖性结合可调节端粒功能。

更新日期：2017-05-25

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