Designing Peptide and Protein Modified Hydrogels: Selecting the Optimal Conjugation Strategy,Journal of the American Chemical Society

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Designing Peptide and Protein Modified Hydrogels: Selecting the Optimal Conjugation Strategy
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2017-05-08 00:00:00 , DOI: 10.1021/jacs.7b00513
Stephanie A. Fisher ₁ , Alexander E.G. Baker ₁ , Molly S. Shoichet ₁

Affiliation

Hydrogels are used in a wide variety of biomedical applications including tissue engineering, biomolecule delivery, cell delivery, and cell culture. These hydrogels are often designed with a specific biological function in mind, requiring the chemical incorporation of bioactive factors to either mimic extracellular matrix or to deliver a payload to diseased tissue. Appropriate synthetic techniques to ligate bioactive factors, such as peptides and proteins, onto hydrogels are critical in designing materials with biological function. Here, we outline strategies for peptide and protein immobilization. We specifically focus on click chemistry, enzymatic ligation, and affinity binding for transient immobilization. Protein modification strategies have shifted toward site-specific modification using unnatural amino acids and engineered site-selective amino acid sequences to preserve both activity and structure. The selection of appropriate protein immobilization strategies is vital to engineering functional hydrogels. We provide insight into chemistry that balances the need for facile reactions while maintaining protein bioactivity or desired release.

中文翻译：

设计肽和蛋白质修饰的水凝胶：选择最佳结合策略

水凝胶可用于多种生物医学应用，包括组织工程，生物分子传递，细胞传递和细胞培养。这些水凝胶通常在设计时考虑到特定的生物学功能，需要化学结合生物活性因子来模拟细胞外基质或将有效载荷运送到患病组织。适当的合成技术将生物活性因子（例如肽和蛋白质）连接到水凝胶上对于设计具有生物学功能的材料至关重要。在这里，我们概述了肽和蛋白质固定化的策略。我们特别专注于点击化学，酶促连接和亲和力结合的瞬时固定化。蛋白质修饰策略已转向使用非天然氨基酸和工程化的位点选择性氨基酸序列进行位点特异性修饰，以保留活性和结构。选择合适的蛋白质固定策略对工程功能水凝胶至关重要。我们提供有关化学的见解，该化学在保持蛋白质生物活性或所需释放的同时平衡了对易反应的需求。

更新日期：2017-05-24

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