Secondary metabolome mining efforts in the myxobacterial multiproducer of natural products, Chondromyces crocatus Cm c5, resulted in the isolation and structure elucidation of crocagins, which are novel polycyclic peptides containing a tetrahydropyrrolo[2,3‐b]indole core. The gene cluster was identified through an approach combining genome analysis, targeted gene inactivation in the producer, and in vitro experiments. Based on our findings, we developed a biosynthetic scheme for crocagin biosynthesis. These natural products are formed from the three C‐terminal amino acids of a precursor peptide and thus belong to a novel class of ribosomally synthesized and post‐translationally modified peptides (RiPPs). We demonstrate that crocagin A binds to the carbon storage regulator protein CsrA, thereby inhibiting the ability of CsrA to bind to its cognate RNA target.

中文翻译：

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crocagins的结构和生物合成：藏红花软骨细胞的多环翻译后修饰的核糖体肽。

天然产物的粘菌多生产者中的次生代谢组挖掘工作，就是Chondromyces crocatus Cm c5，导致了crocagins的分离和结构解析，crocagins是含有四氢吡咯并[2,3-b]吲哚核的新型多环肽。通过将基因组分析，生产者中的靶向基因灭活和体外实验相结合的方法，鉴定了基因簇。根据我们的发现，我们开发了一种用于crocagin生物合成的生物合成方案。这些天然产物是由前体肽的三个C末端氨基酸形成的，因此属于核糖体合成和翻译后修饰的肽（RiPPs）的新型类别。我们证明了crocagin A与碳储存调节蛋白CsrA结合，