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Bioorthogonal double-fluorogenic siliconrhodamine probes for intracellular super-resolution microscopy
Chemical Communications ( IF 4.9 ) Pub Date : 2017-05-11 00:00:00 , DOI: 10.1039/c7cc02212c
E. Kozma 1, 2, 3, 4, 5 , G. Estrada Girona 6, 7, 8, 9, 10 , G. Paci 6, 7, 8, 9, 10 , E. A. Lemke 6, 7, 8, 9, 10 , P. Kele 1, 2, 3, 4, 5
Affiliation  

A series of double-fluorogenic siliconrhodamine probes were synthesized. These tetrazine-functionalized, membrane-permeable labels allowed site-specific bioorthogonal tagging of genetically manipulated intracellular proteins and subsequent imaging using super-resolution microscopy.

中文翻译:

用于细胞内超分辨率显微镜的生物正交双荧光罗丹明探针

合成了一系列的双荧光罗丹明探针。这些四嗪功能化的,可透过膜的标记物允许对基因操作的细胞内蛋白质进行位点特异性生物正交标记,并随后使用超分辨率显微镜进行成像。
更新日期:2017-05-23
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