Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore,Chemical Communications

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Ca-NIR: a ratiometric near-infrared calcium probe based on a dihydroxanthene-hemicyanine fluorophore
Chemical Communications ( IF 4.3 ) Pub Date : 2017-05-15 00:00:00 , DOI: 10.1039/c7cc02418e
Mayeul Collot _{1,

2,

3,

4,

5} , Flavien Ponsot _{1,

2,

3,

4,

5} , Andrey S. Klymchenko _{1,

2,

3,

4,

5}

Affiliation

Fluorescent calcium probes are essential tools for studying the fluctuation of calcium ions in cells. Herein, we developed Ca-NIR, the first ratiometric calcium probe emitting in the near infrared region. This probe arose from the fusion of a BAPTA chelator and a dihydroxanthene-hemicyanine fluorophore. It is efficiently excited with common 630–640 nm lasers and displays two distinct emission bands depending on the calcium concentration (K_d = ∼8 μM). The physicochemical and spectroscopic properties of Ca-NIR allowed for ratiometric imaging of calcium distribution in live cells.

中文翻译：

Ca-NIR：基于二氢黄嘌呤-半花菁荧光团的比例式近红外钙探针

荧光钙探针是研究细胞中钙离子波动的重要工具。本文中，我们开发了Ca-NIR，这是第一种在近红外区域发射的比例钙探针。该探针源自BAPTA螯合剂和二氢黄嘌呤-半花菁荧光团的融合。它可以通过普通的630–640 nm激光有效激发，并根据钙浓度显示两个不同的发射带（K _d =〜8μM）。Ca-NIR的理化性质和光谱性质允许对活细胞中钙分布进行比例成像。

更新日期：2017-05-22

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