DCEO Biotechnology: Tools To Design, Construct, Evaluate, and Optimize the Metabolic Pathway for Biosynthesis of Chemicals,Chemical Reviews

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DCEO Biotechnology: Tools To Design, Construct, Evaluate, and Optimize the Metabolic Pathway for Biosynthesis of Chemicals
Chemical Reviews ( IF 51.4 ) Pub Date : 2017-04-26 00:00:00 , DOI: 10.1021/acs.chemrev.6b00804
Xiulai Chen _{1,

2} , Cong Gao _{1,

2} , Liang Guo _{1,

2} , Guipeng Hu _{1,

2} , Qiuling Luo _{1,

2} , Jia Liu _{1,

2} , Jens Nielsen _{3,

4} , Jian Chen _{1,

2} , Liming Liu _{1,

2,

3}

Affiliation

Chemical synthesis is a well established route for producing many chemicals on a large scale, but some drawbacks still exist in this process, such as unstable intermediates, multistep reactions, complex process control, etc. Biobased production provides an attractive alternative to these challenges, but how to make cells into efficient factories is challenging. As a key enabling technology to develop efficient cell factories, design-construction-evaluation-optimization (DCEO) biotechnology, which incorporates the concepts and techniques of pathway design, pathway construction, pathway evaluation, and pathway optimization at the systems level, offers a conceptual and technological framework to exploit potential pathways, modify existing pathways and create new pathways for the optimal production of desired chemicals. Here, we summarize recent progress of DCEO biotechnology and examples of its application, and provide insights as to when, what and how different strategies should be taken. In addition, we highlight future perspectives of DCEO biotechnology for the successful establishment of biorefineries.

中文翻译：

DCEO生物技术：设计，构建，评估和优化化学品生物合成代谢途径的工具

化学合成是大规模生产许多化学药品的公认方法，但是该方法仍然存在一些缺点，例如不稳定的中间体，多步反应，复杂的过程控制等。生物基生产为这些挑战提供了有吸引力的替代方法，但是如何使细胞成为高效的工厂具有挑战性。作为开发高效细胞工厂的关键使能技术，设计-构建-评估-优化（DCEO）生物技术在系统级别融合了途径设计，途径构建，途径评估和途径优化的概念和技术，提供了一种概念和技术框架，以开发潜在途径，修改现有途径并为最佳生产所需化学品创造新途径。这里，我们总结了DCEO生物技术的最新进展及其应用示例，并提供了有关何时，什么以及如何采用不同策略的见解。此外，我们重点介绍了DCEO生物技术在成功建立生物炼油厂方面的未来前景。

更新日期：2017-06-28

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