Single-Cell Genomics Sequencing the genome of single cells gives insight into issues such as cell-to-cell heterogeneity and genome instability. Key to single-cell sequencing techniques are whole-genome amplification (WGA) methods that provide sufficient DNA for next-generation sequencing. Current WGA methods have been hampered by low accuracy and spatial resolution of gene copy numbers and by low amplification fidelity. Chen et al. report an improved single-cell WGA method, Linear Amplification via Transposon Insertion (LIANTI). The DNA is randomly fragmented by Tn5 transposition of a transposon that includes a T7 promoter, which allows linear amplification. The authors used the method to determine the spectrum of single-nucleotide variations in a single human cell after ultraviolet radiation. Science , this issue p. [189][1] [1]: /lookup/doi/10.1126/science.aak9787

中文翻译：

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制作一个无偏见的图书馆

单细胞基因组学 对单细胞基因组进行测序可以深入了解细胞间异质性和基因组不稳定性等问题。单细胞测序技术的关键是全基因组扩增 (WGA) 方法，可为下一代测序提供足够的 DNA。当前的 WGA 方法受到基因拷贝数的低准确性和空间分辨率以及低扩增保真度的阻碍。陈等人。报告了一种改进的单细胞 WGA 方法，通过转座子插入的线性放大 (LIANTI)。DNA 被包含 T7 启动子的转座子的 Tn5 转座随机片段化，从而允许线性扩增。作者使用该方法确定了紫外线辐射后单个人类细胞中单核苷酸变异的光谱。科学，这个问题 p。[189][1][1]：/lookup/doi/10。