Vibrational Probes: From Small Molecule Solvatochromism Theory and Experiments to Applications in Complex Systems,Accounts of Chemical Research

当前位置： X-MOL 学术 › Acc. Chem. Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Vibrational Probes: From Small Molecule Solvatochromism Theory and Experiments to Applications in Complex Systems
Accounts of Chemical Research ( IF 16.4 ) Pub Date : 2017-03-27 00:00:00 , DOI: 10.1021/acs.accounts.7b00002
Bartosz Błasiak _{1,

2} , Casey H. Londergan ₃ , Lauren J. Webb ₄ , Minhaeng Cho _{1,

2}

Affiliation

The vibrational frequency of a chosen normal mode is one of the most accurately measurable spectroscopic properties of molecules in condensed phases. Accordingly, infrared absorption and Raman scattering spectroscopy have provided valuable information on both distributions and ensemble-average values of molecular vibrational frequencies, and these frequencies are now routinely used to investigate structure, conformation, and even absolute configuration of chemical and biological molecules of interest. Recent advancements in coherent time-domain nonlinear vibrational spectroscopy have allowed the study of heterogeneous distributions of local structures and thermally driven ultrafast fluctuations of vibrational frequencies. To fully utilize IR probe functional groups for quantitative bioassays, a variety of biological and chemical techniques have been developed to site-specifically introduce vibrational probe groups into proteins and nucleic acids. These IR-probe-labeled biomolecules and chemically reactive systems are subject to linear and nonlinear vibrational spectroscopic investigations and provide information on the local electric field, conformational changes, site–site protein contacts, and/or function-defining features of biomolecules.

中文翻译：

振动探针：从小分子溶剂变色理论和实验到复杂系统中的应用

选定的正常模式的振动频率是分子在凝聚相中可最准确测量的光谱性质之一。因此，红外吸收和拉曼散射光谱学已经提供了有关分子振动频率的分布和总体平均值的有价值的信息，并且现在这些频率被常规地用于研究感兴趣的化学和生物分子的结构，构象，甚至是绝对构型。相干时域非线性振动光谱学的最新进展允许研究局部结构的不均匀分布以及振动频率的热驱动超快波动。为了充分利用IR探针官能团进行定量生物测定，已经开发了多种生物和化学技术以将振动探针基团特异性地引入蛋白质和核酸中。这些经过IR探针标记的生物分子和化学反应系统需要进行线性和非线性振动光谱研究，并提供有关局部电场，构象变化，位点间蛋白质接触和/或生物分子功能定义特征的信息。

更新日期：2017-03-27

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11