DNA Nanotechnology A wide variety of DNA nanostructures have been assembled by folding long DNA single strands with short DNA staples. However, such structures typically need annealing at elevated temperatures in order to form. To accommodate the formation of such structures in living cells, Praetorius and Dietz developed an approach in which custom protein staples based on transcription activator–like effector proteins fold double-stranded DNA templates (see the Perspective by Douglas). The structures folded into user-defined geometric shapes on the scale of tens to hundreds of nanometers. These nanostructures could self-assemble at room temperature in physiological buffers. Science , this issue p. [eaam5488][1]; see also p. [1261][2] [1]: /lookup/doi/10.1126/science.aam5488 [2]: /lookup/doi/10.1126/science.aam9541

中文翻译：

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蛋白质折叠的DNA纳米结构

DNA 纳米技术 通过折叠长 DNA 单链和短 DNA 订书钉，已经组装了各种各样的 DNA 纳米结构。然而，这种结构通常需要在升高的温度下退火才能形成。为了适应活细胞中此类结构的形成，Praetorius 和 Dietz 开发了一种方法，其中基于转录激活因子样效应蛋白的定制蛋白质主食折叠双链 DNA 模板（参见 Douglas 的观点）。这些结构折叠成几十到几百纳米尺度的用户定义的几何形状。这些纳米结构可以在室温下在生理缓冲液中自组装。科学，这个问题 p。[eaam5488][1]; 另见第。[1261][2][1]：/lookup/doi/10.1126/science.aam5488 [2]：/lookup/doi/10.1126/science.aam9541