Cross-Linked Micellar Spherical Nucleic Acids from Thermoresponsive Templates,Journal of the American Chemical Society

当前位置： X-MOL 学术 › J. Am. Chem. Soc. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Cross-Linked Micellar Spherical Nucleic Acids from Thermoresponsive Templates
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2017-03-20 , DOI: 10.1021/jacs.6b13359
Resham J. Banga ₁ , Brian Meckes ₁ , Suguna P. Narayan ₁ , Anthony J. Sprangers ₁ , SonBinh T. Nguyen ₁ , Chad A. Mirkin ₁

Affiliation

A one-pot synthesis of micellar spherical nucleic acid (SNA) nanostructures using Pluronic F127 as a thermoresponsive template is reported. These novel constructs are synthesized in a chemically straightforward process that involves intercalation of the lipid tails of DNA amphiphiles (CpG motifs for TLR-9 stimulation) into the hydrophobic regions of Pluronic F127 micelles, followed by chemical cross-linking and subsequent removal of non-cross-linked structures. The dense nucleic acid shell of the resulting cross-linked micellar SNA enhances their stability in physiological media and facilitates their rapid cellular internalization, making them effective TLR-9 immunomodulatory agents. These constructs underscore the potential of SNAs in regulating immune response and address the relative lack of stability of noncovalent constructs.

中文翻译：

来自热响应模板的交联胶束球形核酸

报道了使用 Pluronic F127 作为热响应模板的胶束球形核酸 (SNA) 纳米结构的一锅法合成。这些新型构建体是在化学上简单的过程中合成的，该过程涉及将 DNA 两亲物的脂质尾部（用于 TLR-9 刺激的 CpG 基序）插入 Pluronic F127 胶束的疏水区域，然后进行化学交联并随后去除非交联结构。所得交联胶束 SNA 的致密核酸壳增强了它们在生理介质中的稳定性并促进它们的快速细胞内化，使其成为有效的 TLR-9 免疫调节剂。这些构建体强调了 SNA 在调节免疫反应方面的潜力，并解决了非共价构建体相对缺乏稳定性的问题。

更新日期：2017-03-20

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11