研究领域

生物核磁，分析化学，分子生物学和结构生物学

蛋白质（-蛋白质/核酸）复合物的结构与功能研究：

（1）生物大分子复合物的结构与动力学的新方法研究

        在单体的NMR结构基础上，应用NMR方法(NOE, CSP, PRE, PCS, CEST，etc), EPR, HDX-MS, SAXS/SANS等方法，获取单体间的距离、取向和界面位点等不同信息，发展新的复合物结构研究方法。

（2）蛋白质-蛋白质/核酸复合物的结构与功能研究

        当前研究课题有：SP_0782-ssDNA, CV0373-dsRNA, TGIF1(HD)-DNA, INSM1(ZF)-DNA等课题。

（3）生物大分子复合物的结构与动力学的MS研究

近期论文

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1.         Shuangli Li, Theresa A. Ramelot, Michael A. Kennedy, Maili Liu^*, and Yunhuang Yang^*," Chemical Shift Assignments of the Homodimer Protein SP_0782 (7-79) from Streptococcus pneumoniae ", Biomolecular NMR Assignments, 2016.

2.         李双利, 朱勤俊, 刘买利, 杨运煌^*, "蛋白质分子核磁共振谱峰的特性及其化学位移归属的一些技巧", 波谱学杂志，2016。

3.         Bo Li, Xudong Wang, Yali Guo, Anam Iqbal, Weisheng Liu, Wenwu Qin, Yaping Dong, Wu Li,   Shizhen Chen, Xin Zhou and Yunhuang Yang*, "One-pot Synthesis Polyamines Improved Magnetism and Fluorescence Fe3O4-Carbon Dots Hybrid NPs for Dual Modal Imaging", Dalton transaction,45: 5484-5491, 2016.

4.         John Everett, Roberto Tejero, Sarath B.K. Murthy, Thomas B. Acton, James M. Aramini, Michael C. Baran, Jordi Benach, John R. Cort, Alexander Eletsky, Farhad Forouhar, Rongjin Guan, Alexandre P. Kuzin, Hsiau-Wei Lee, Gaohua Liu, Rajeswari Mani, Binchen Mao, Jeffery L. Mills, Alexander F. Montelione, Kari Pederson, Robert Powers, Theresa Ramelot, Paolo Rossi, Jayaraman Seetharaman, David Snyder, G.V.T. Swapna, Sergey M. Vorobiev, Yibing Wu, Rong Xiao, Yunhuang Yang, Cheryl H. Arrowsmith, John Hunt, Michael A. Kennedy, James H. Prestegard, Thomas Szyperski, Liang Tong andGaetano T. Montelione, "A Community Resource of Experimental Data for NMR – X-ray Crystal Structure Pairs", Protein Science, 25: 30-45, 2016.

5.         Yunhuang Yang, Theresa A. Ramelot, Hsiau-Wei Lee, Rong Xiao, John K. Everett, Gaetano T. Montelione, James H. Prestegard, and Michael A. Kennedy. Solution structure of a C-terminal fragment (175-257) of CV_0373 protein from Chromobacterium violaceum adopts a winged helix-turn-helix (wHTH) fold. Journal of Biomolecular NMR, 60:197-202, 2014.

6.         Yunhuang Yang, Theresa A. Ramelot, Hsiau-Wei Lee, Rong Xiao, John K. Everett, Gaetano T. Montelione, James H. Prestegard, and Michael A. Kennedy. Solution structure of the free Za domain of human DLM-1 (ZBP1/DAI), a Z-DNA binding domain. Journal of Biomolecular NMR, 60:189-195, 2014.

7.         Sherif I. Elshahawi, Theresa A. Ramelot, Jayaraman Seetharaman, Jing Chen, Shanteri Singh, Yunhuang Yang, Kari Pederson, Madan K. Kharel, Rong Xiao, Thomas B. Acton, John K. Everett, Ragothaman M. Yennamalli, Fengbin Wang, James H. Prestegard, Scott Lew, Liang Tong, Gaetano T. Montelione, Michael A. Kennedy, Craig A. Bingman, Haining Zhu, George N. Phillips Jr, and Jon S. Thorson. Structure-Guided Functional Characterization of Enediyne Self-Sacrifice Resistance Proteins, CalU16 and CalU19. ACS Chemical Biology, 9: 2347-2358, 2014.

8.         Yunhuang Yang, Theresa A. Ramelot, Shuisong Ni, Robert M. McCarrick, and Michael A. Kennedy. Applications of NMR-based PRE and EPR-based DEER spectroscopy to homodimer chain exchange characterization and structure determination. Methods in Molecular Biology, 1091: 215-227, 2014.

9.         Theresa A. Ramelot, Yunhuang Yang, Indra D. Sahu, Hsiau-Wei Lee, Rong Xiao, Gary A. Lorigan, Gaetano T. Montelione, and Michael A. Kennedy. NMR structure and MD simulations of the AAA protease intermembrane space domain indicates peripheral membrane localization within the hexaologome. FEBS Letter, 587(21):3522-3528, 2013.

10.     Yunhuang Yang, Theresa A. Ramelot, Shuisong Ni, Robert M. McCarrick, and Michael A. Kennedy. Measurement of rate constants for homodimer subunit exchange using double electron-electron resonance and paramagnetic relaxation enhancements. Journal of Biomolecular NMR, 55, 47-58, 2013.

11.     Theresa A. Ramelot, Paolo Rossi, Farhad Forouhar, Hsiau-Wei Lee, Yunhuang Yang, Shuisong Ni, Sarah Unser, Scott Lew, Jayararaman Seetharaman, Rong Xiao, Thomas B. Acton, John K. Everett, James H. Prestegard, John F. Hunt, Gaetano T. Montelione, and Michael A. Kennedy. Structure of a specialized acyl carrier protein essential for lipid A biosynthesis with very long chain fatty acids in open and closed conformations. Biochemistry, 51, 7239-7249, 2012.

12.     Antonio Rosatosend, James M. Aramini, Cheryl Arrowsmith, Anurag Bagaria, David Baker, Andrea Cavalli, Jurgen F. Doreleijers, Alexander Eletsky, Andrea Giachetti, Paul Guerry, Aleksandras Gutmanas, Peter Güntert, Yunfen He, Torsten Herrmann, Yuanpeng J. Huang, Victor Jaravine, Hendrik R.A. Jonker, Michael A. Kennedy, Oliver F. Lange, Gaohua Liu, Thérèse E. Malliavin, Rajeswari Mani, Binchen Mao, Gaetano T. Montelione, Michael Nilges, Paolo Rossi, Gijs van der Schot, Harald Schwalbe, Thomas A. Szyperski, Michele Vendruscolo, Robert Vernon, Wim F. Vranken, Sjoerd de Vries, Geerten W. Vuister, Bin Wu, Yunhuang Yang, and Alexandre M.J.J. Bonvin. Blind testing of routine, fully automated determination of protein structures from NMR data. Structure, 20, 227-236, 2012.

13.     Yunhuang Yang, Theresa A. Ramelot, John R. Cort, Maite Garcia, Adelinda Yee, Cheryl H. Arrowsmith, and Michael A. Kennedy. Solution NMR structure of hypothetical protein CV_2116 encoded by a viral prophage element in Chromobacterium violaceum. International Journal of Molecular Sciences, 13, 7354-7364, 2012.

14.     Yunhuang Yang, Theresa A. Ramelot, John R. Cort, Huang Wang, Colleen Ciccosanti, Mei Jiang, Haleema Janjua, Thomas B. Acton, Rong Xiao, John K. Everett, Gaetano T. Montelione, and Michael A. Kennedy. Solution NMR structure of Dsy0195 homodimer from Desulfitobacterium hafniense: first structure representative of the YabP domain family of proteins involved in spore coat assembly. Journal of Structural and Functional Genomics; 12, 175-179, 2011.

15.     Theresa A. Ramelot, Yunhuang Yang, Rong Xiao, Thomas B. Acton, John K. Everett, Gaetano T. Montelione, and Michael A. Kennedy. Solution NMR structure of BT_0084, a conjugative transposon lipoprotein from Bacteroides thetaiotamicron. Proteins: Structure, Function, and Bioinformatics, 80, 667-670, 2011.

16.     Erik A. Feldmann, Theresa A. Ramelot, Yunhuang Yang, Rong Xiao, Thomas B. Acton, John K. Everett, Gaetano T. Montelione, and Michael A. Kennedy. Solution NMR structure of Asl3597 from Nostoc sp. PCC7120, the first structure from protein domain family PF12095, reveals a novel fold. Proteins: Structure, Function, and Bioinformatics, 80, 671-675, 2011.

17.     Yunhuang Yang, Theresa A. Ramelot, John R. Cort, Dongyan Wang, Colleen Ciccosanti, Keith Hamilton, Rajesh Nair, Burkhard Rost, Thomas B. Acton, Rong Xiao, John K. Everett, Gaetano T. Montelione, and Michael A. Kennedy. Solution NMR structure of photosystem II reaction center protein Psb28 from Synechocystis sp. strain PCC 6803. Proteins: Structure, Function, and Bioinformatics, 79, 340-344, 2011.

18.     Yunhuang Yang, Theresa A. Ramelot, Robert M. McCarrick, Shuisong Ni, Erik A. Feldmann, John R. Cort, Huang Wang, Colleen Ciccosanti, Mei Jiang, Haleema Janjua, Thomas B. Acton, Rong Xiao, John K. Everett, Gaetano T. Montelione, and Michael A. Kennedy. Combining NMR and EPR methods for homodimer protein structure determination. Journal of American Chemical Society, 132, 11910-11913, 2010.

19.     Arun Sivashanmugam, Yunhuang Yang, Victoria Murray, Christopher McCullough, Bin Chen, Xuefeng Ren, Qianqian Li, and Jianjun Wang. Structural basis of human high-density lipoprotein formation and assembly at sub nanometer resolution. Methods in Cell Biology, 90, 327-364, 2008.

20.     Xuefeng Ren, Yunhuang Yang, Tracey Neville, David Hoyt, Daniel Sparks, and Jianjun Wang. A complete backbone spectral assignment of human apolipoprotein AI on a 38 kDa preb-HDL (Lp1-AI) particle. Biomolecular NMR Assignments, 1, 69-71, 2007.

21.     Yunhuang Yang, David Hoyt, and Jianjun Wang. A complete NMR spectral assignment of the lipid-free mouse apolipoprotein A-I (apoAI) C-terminal truncation mutant, apoAI (1-216). Biomolecular NMR Assignments, 1, 109-111, 2007.

22.     Xuefeng Ren, Arun Sivashanmugam, Bin Chen, Congmin Li, Yunhuang Yang, Tracey Neville, David Hoyt, W. Gray Jerome, Daniel Sparks, Qianqian Li, and Jianjun Wang. NMR structural studies of human apolipoprotein A-I at the lipid-free and HDL-associated states: A progress report. Functional and Structural Biology on the Lipo-network, 65-93, 2006.

23.     Guoyun Bai, Yanfang Cui, Yunhuang Yang, Chaohui Ye, and Maili Liu. A competitive low-affinity binding model for determining the mutual and specific sites of two ligands on protein. Journal of Pharmaceutical and Biomedical Analysis, 38, 588-593, 2005.

24.     Xiaochuan Wang, Zhenghui Hu, Zhengyu Fang, Yue Feng, Yunhuang Yang, Qun Wang, Xiaowei Tang, Yigen Wu, and Jianzhi Wang. Correlation of Alzheimer-like tau hyperphosphorylation and fMRI bold intensity. Current Alzheimer Research, 1, 143-148, 2004.

25.     Yunhuang Yang, Guoyun Bai, Xu Zhang, Chaohui Ye, and Maili Liu. ¹H NMR spectroscopic evidence of interaction between ibuprofen and lipoproteins in human blood plasma. Analytical Biochemistry, 324, 292-297, 2004.

26.     Guoping Yan, Renxi Zhuo, Yunhuang Yang, Liyun Li, Maili Liu, and Chaohui Ye. Tumor-selective macromolecular MRI contrast agents. Journal of Bioactive and Compatible Polymers, 17:139-151, 2002.