个人简介

BS Chem., Bradley U, 1977; PhD Biochemistry, with J. Ivan Legg, Washington State U, 1983; Postdoc with Michael Smith, U British Columbia, 1983-1986; Postdoc with Robert JP Williams, Oxford. 1986-1988; DuPont Young Faculty Award; Morrow Young Faculty Award; NIH Pioneer Award, 2006; National Science Foundation Program Director 2013-2014

研究领域

Analytical/Biochemistry & Chemical Biology

Protein Biophysics Emphasizing Studies in Living Cells

近期论文

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Smith AE, Zhou Z, Pielak GJ. 2015. Hydrogen exchange of disordered proteins in Escherichia coli. Protein Science, 24: in press. Monteith WB, Cohen RD, Smith AE, Guzman-Cisneros E. 2015. Quinary structure modulates protein stability in cells. Proceedings of the National Academy of Sciences of the United States of America, in press. Smith AE, Zhang Z, Pielak GJ, Li C 2015. NMR studies of protein folding and binding in cells and cell-like environments. Current Opinion in Structural Biology. 30 7-16. Sarkar M, Pielak GJ 2014. An osmolyte mitigates the destabilizing effect of protein crowding. Protein Science 23: 1161-1164. Cover article. Monteith WB, Pielak GJ 2014. Residue level quantification of protein stability in living cells. Proceedings of the National Academy of Sciences of the United States of America 111: 11335-11340. Featured by Faculty of 1000. Theillet F-X, Binolfi A, Frembgen-Kesner T, Hingorani K, Sarkar M, Kyne C, Li C, Crowley P, Gierasch L, Pielak G, Elcock A, Gershenson A, Selenko P. 2014. Physicochemical properties of cells and their effects on intrinsically disordered proteins (IDPs). Chemical Reviews 13: 6661-6714. Xu G, Ye Y, Liu X, Cao S, Wu Q, Cheng K, Liu M, Pielak GJ, Li C. 2014. Strategies for Protein NMR in Escherichia coli. Biochemistry 53: 1971-1981. Sarkar M, Lu J, Pielak GJ. 2014. Protein-crowder charge and protein stability. Biochemistry 53: 1601-1606. Tyrrell J, McGinnis JL, Weeks KM, Pielak GJ. 2013. The cellular environment stabilizes adenine riboswitch RNA structure. Biochemistry, 52: 8777–8785. Featured by Faculty of 1000. Sarkar M, Smith AE, Pielak GJ. 2013. Impact of reconstituted cytosol on protein stability. Proceedings of the National Academy of Sciences of the United States of America 110: 19342-19347. Featured by Faculty of 1000. Smith AE, Sarkar M, Young GB, Pielak GJ. 2013. Amide proton exchange of a dynamic loop in cell extracts. Protein Science 22: 1313-1319. Ye Y, Liu X, Zhang Z, Wu Q, Jiang B, Jiang L, Zhang X, Liu M, Pielak GJ, C Li C. 2013. 19F NMR as a probe of cytoplasmic viscosity and weak protein interactions in living cells. Chemistry--A European Journal 19: 12705-12710. Sarkar M, Li C, Pielak GJ. 2013. Soft interactions and crowding. Biophysical Reviews 5: 187-194. Gary J. Pielak 1/18/15 Benton LA, Smith AE, Young GB, Pielak GJ. 2012. Unexpected effects of macromolecular crowding on protein stability. Biochemistry 51: 9773-9775. Wang Y, Sarkar M, Smith AE, Krois AS, Pielak, GJ. 2012. Macromolecular crowding and protein stability. Journal of the American Chemical Society 134: 16614–16618. Wang Y, Benton L, Singh V, Pielak GJ. 2012. Disordered protein diffusion under crowded conditions. Journal of Physical Chemistry Letters 3: 2703–2706. Wang Y, Li C, Pielak GJ. 2012. In-cell protein magnetic resonance spectroscopy. Chinese Journal of Magnetic Resonance, 29: 475-488. Pielak GJ, Tian F. 2012. Membrane proteins, magic-angle spinning, and in-cell NMR. Proceedings of the National Academy of Sciences of the United States of America 109: 4715-4716. Zigoneanu IG, Pielak GJ 2012. Interaction of α-synuclein and a cell penetrating fusion peptide with higher eukaryotic cell membranes assessed by 19F NMR. Molecular Pharmaceutics 9: 1024-1029. Zigoneanu IG, Yang YJ, Krois AS, Haque Md E, Pielak GJ. 2011. Interaction of α-synuclein and its A30P variant with vesicles of composition similar to mitochondrial membranes. Biochimica et Biophysica Acta 1818: 512-519. Fu R, Wang X, Li C, Santiago-Miranda A, Pielak GJ, Tian F. 2011. In situ Structural characterization of a recombinant protein in native Escherichia coli membranes with solid-state MAS NMR. Journal of the American Chemical Society 133: 12370-12373. Schlesinger AP, Wang Y, Tadeo X, Millet O, Pielak GJ. 2011 Macromolecular crowding fails to fold a globular protein in cells. Journal of the American Chemical Society 133: 8082-8085. Miklos AC, Sarkar M, Wang Y, Pielak GJ. 2011. Proteins tune protein stability. Journal of the American Chemical Society 133: 7116–7120. Featured by Faculty of 1000. Miklos AC, Li C, Sorrell CD, Lyon LA, Pielak GJ. 2011. An upper limit for macromolecular crowding effects. BMC Biophysics 4:13. Barnes CO, Monteith WB, Pielak GJ. 2011. Internal and global protein motion assessed with a fusion construct and in-cell NMR. ChemBioChem 12: 390-391. Barnes CO, Pielak GJ. 2011. In-cell NMR and protein leakage. Proteins: Structure, Function, and Bioinformatics 79: 347-351.