Brooks, Donald - University of British Columbia

个人简介

B.Sc., British Columbia (1964); M.Sc., British Columbia (C.P.S. Taylor, 1967); Ph.D., Oregon (G.V.F. Seaman, 1971); Postdoctoral, Weizmann Institute (A. Silberberg, 1971-72) and Cambridge (D.A. Haydon, 1972-74).

研究领域

It is known that grafted polymer chains have a strong effect on the interaction of surfaces with blood and biological fluids. Our approach to developing biocompatible surfaces is to synthesize relatively large, surfactant-free solid polymer latex beads based on polystyrene and provide them with a reactive copolymer shell to allow grafting of acrylate-based monomers and co-monomers. These provide model, polymer-bearing surfaces which are then evaluated via biocompatibility reactions with proteins and cells. By assaying the number of reacted surface groups and the amount of monomer incorporated the molecular weights of the grafts are determined. The surfaces are then characterized by traditional (NMR), surface chemical (XPS) and non-traditional approaches (particle electrophoresis; contact angles in phase separated aqueous two phase mixtures) to provide unique descriptions of the grafted chain phase and its effects on biocompatibility. The grafted beads are further evaluated as high resolution chromatographic media by packing them in HPLC columns and measuring plate heights and partit ion coefficients for biomolecules, the latter parameter providing a further characterization of the grafted layer. The grafted surfaces are also being used to develop a thermodynamic method for measuring the accessibility of various locations in the grafted layer to dissolved macromolecules as a tool to be applied to cell surface structure definition. The particles are further used in studies of the colloidal stability of suspensions and the effects of grafted chain composition on depletion or bridging destabilization induced by dissolved macromolecules. Work on macromolecular stabilizing agents for cells via adsorption of copolymers to cell surfaces, is being extended towards development of disaggregation agents for whole blood. The effects of such agents on the rheological behaviour of blood from diabetes, stroke and heart attack patients is being studied out as a possible treatment for the abnormally high red cell aggregation known to be a risk fact or in these patients. A significant enhancement in sensitivity to cell surface properties is being sought in work on the development of cell isolation procedures utilizing partition in phase separated two polymer solutions. The separations depend on the surface free energy of cells in the phases and on a stochastic factor that is likely associated with the gravity-driven flows present during phase separation. To circumvent these effects we are synthesizing copolymers with which to make equal density phases to allow experiments of the type we performed previously on the Space Shuttle.

近期论文

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Kizhakkedathu, J. N. ; Janzen, J. ; Le, Y. ; Kainthan, R. K. ; Brooks, D. E. Poly(Oligo(Ethylene Glycol)Acrylamide) Brushes By Surface Initiated Polymerization: Effect Of Macromonomer Chain Length On Brush Growth And Protein Adsorption From Blood Plasma. Langmuir 2009, 25, 3794-3801.BibTex Zou, Y. Q. ; Rossi, N. A. A. ; Kizhakkedathu, J. N. ; Brooks, D. E. Barrier Capacity Of Hydrophilic Polymer Brushes To Prevent Hydrophobic Interactions: Effect Of Graft Density And Hydrophilicity. Macromolecules 2009, 42, 4817-4828.BibTex Mugabe, C. ; Hadaschik, B. A. ; Kainthan, R. K. ; Brooks, D. E. ; So, A. I. ; Gleave, M. E. ; Burt, H. M. Paclitaxel Incorporated In Hydrophobically Derivatized Hyperbranched Polyglycerols For Intravesical Bladder Cancer Therapy. Bju International 2009, 103, 978-986.BibTex Zou, Y. Q. ; Kizhakkedathu, J. N. ; Brooks, D. E. Surface Modification Of Polyvinyl Chloride Sheets Via Growth Of Hydrophilic Polymer Brushes. Macromolecules 2009, 42, 3258-3268.BibTex 2008 Kainthan, R. K. ; Brooks, D. E. Unimolecular Micelles Based On Hydrophobically Derivatized Hyperbranched Polyglycerols: Biodistribution Studies. Bioconjugate Chemistry 2008, 19, 2231-2238.BibTex Kainthan, R. K. ; Mugabe, C. ; Burt, H. M. ; Brooks, D. E. Unimolecular Micelles Based On Hydrophobically Derivatized Hyperbranched Polyglycerols: Ligand Binding Properties. Biomacromolecules 2008, 9, 886-895.BibTex Ranganathan, K. ; Deng, R. ; Kainthan, R. K. ; Wu, C. ; Brooks, D. E. ; Kizhakkedathu, J. N. Synthesis Of Thermoresponsive Mixed Arm Star Polymers By Combination Of Raft And Atrp From A Multifunctional Core And Its Self-Assembly In Water. Macromolecules 2008, 41, 4226-4234.BibTex Zou, Y. Q. ; Brooks, D. E. ; Kizhakkedathu, J. N. A Novel Functional Polymer With Tunable Lcst. Macromolecules 2008, 41, 5393-5405.BibTex Zhang, J. G. ; Krajden, O. B. ; Kainthan, R. K. ; Kizhakkedathu, J. N. ; Constantinescu, I. ; Brooks, D. E. ; Gyongyossy-Issa, M. I. C. Conjugation To Hyperbranched Polyglycerols Improves Rgd-Mediated Inhibition Of Platelet Function In Vitro. Bioconjugate chemistry 2008, 19, 1241-7.BibTex 2007 Kainthan, R. K. ; Hester, S. R. ; Levin, E. ; Devine, D. V. ; Brooks, D. E. In Vitro Biological Evaluation Of High Molecular Weight Hyperbranched Polyglycerols. Biomaterials 2007, 28, 4581-4590.BibTex Kainthan, R. K. ; Brooks, D. E. In Vivo Biological Evaluation Of High Molecular Weight Hyperbranched Polyglycerols. Biomaterials 2007, 28, 4779-4787.BibTex Coad, B. R. ; Kizhakkedathu, J. N. ; Haynes, C. A. ; Brooks, D. E. Synthesis Of Novel Size Exclusion Chromatography Support By Surface Initiated Aqueous Atom Transfer Radical Polymerization. Langmuir 2007, 23, 11791-11803.BibTex Coad, B. R. ; Steels, B. M. ; Kizhakkedathu, J. N. ; Brooks, D. E. ; Haynes, C. A. The Influence Of Grafted Polymer Architecture And Fluid Hydrodynamics On Protein Separation By Entropic Interaction Chromatography. Biotechnology and Bioengineering 2007, 97, 574-587.BibTex 2006 Kainthan, R. K. ; Gnanamani, M. ; Ganguli, M. ; Ghosh, T. ; Brooks, D. E. ; Maiti, S. ; Kizhakkedathu, J. N. Blood Compatibility Of Novel Water Soluble Hyperbranched Polyglycerol-Based Multivalent Cationic Polymers And Their Interaction With Dna. Biomaterials 2006, 27, 5377-5390.BibTex 2005 Kumar, K. R. ; Brooks, D. E. Comparison Of Hyperbranched And Linear Polyglycidol Unimolecular Reverse Micelles As Nanoreactors And Nanocapsules. Macromolecular Rapid Communications 2005, 26, 155-159.BibTex Pang, P. ; Koska, J. ; Coad, B. R. ; Brooks, D. E. ; Haynes, C. A. Entropic Interaction Chromatography: Separating Proteins On The Basis Of Size Using End-Grafted Polymer Brushes. Biotechnology and Bioengineering 2005, 90, 1-13.BibTex 2004 Kumar, K. R. ; Kizhakkedathu, J. N. ; Brooks, D. E. Atom Transfer Radical Polymerization Using Multidentate Amine Ligands Supported On Soluble Hyperbranched Polyglycidol. Macromolecular Chemistry and Physics 2004, 205, 567-573.BibTex Goodman, D. ; Kizhakkedathu, J. N. ; Brooks, D. E. Attractive Bridging Interactions In Dense Polymer Brushes In Good Solvent Measured By Atomic Force Microscopy. Langmuir 2004, 20, 2333-2340.BibTex Goodman, D. ; Kizhakkedathu, J. N. ; Brooks, D. E. Evaluation Of An Atomic Force Microscopy Pull-Off Method For Measuring Molecular Weight And Polydispersity Of Polymer Brushes: Effect Of Grafting Density. Langmuir 2004, 20, 6238-6245.BibTex Kizhakkedathu, J. N. ; Norris-Jones, R. ; Brooks, D. E. Synthesis Of Well-Defined Environmentally Responsive Polymer Brushes By Aqueous Atrp. Macromolecules 2004, 37, 734-743.BibTex Yang, C. ; Kizhakkedathu, J. N. ; Brooks, D. E. ; Jin, F. ; Wu, C. Laser-Light-Scattering Study Of Internal Motions Of Polymer Chains Grafted On Spherical Latex Particles. Journal of Physical Chemistry B 2004, 108, 18479-18484.BibTex Goodman, D. ; Kizhakkedathu, J. N. ; Brooks, D. E. Molecular Weight And Polydispersity Estimation Of Adsorbing Polymer Brushes By Atomic Force Microscopy. Langmuir 2004, 20, 3297-3303.BibTex Janzen, J. ; Le, Y. ; Kizhakkedathu, J. N. ; Brooks, D. E. Plasma Protein Adsorption To Surfaces Grafted With Dense Homopolymer And Copolymer Brushes Containing Poly(N-Isopropylacrylamide). Journal of Biomaterials Science-Polymer Edition 2004, 15, 1121-1135.BibTex Kizhakkedathu, J. N. ; Kumar, K. R. ; Goodman, D. ; Brooks, D. E. Synthesis And Characterization Of Well-Defined Hydrophilic Block Copolymer Brushes By Aqueous Atrp. Polymer 2004, 45, 7471-7489.BibTex 2003 Kizhakkedathu, J. N. ; Brooks, D. E. Synthesis Of Poly(N,n-Dimethylacrylamide) Brushes From Charged Polymeric Surfaces By Aqueous Atrp: Effect Of Surface Initiator Concentration. Macromolecules 2003, 36, 591-598.BibTex Kizhakkedathu, J. N. ; Goodman, D. ; Brooks, D. E. Synthesis Of Poly(N,n-Dimethylacrylamide) Brushes From Functionalized Latex Surfaces By Aqueous Atom Transfer Radical Polymerization. In Advances in Controlled/Living Radical Polymerization; Matyjaszewski, K. ; Advances in Controlled/Living Radical Polymerization; Amer Chemical Soc: Washington, 2003; Vol. 854, pp. 316-330.BibTex