个人简介

PhD - Microbiology McGill University, 1997 MS - Geography McGill University, 1991 BSc - Biology Dalhousie University, 1988

研究领域

Microbiology

1. Extremophiles:16S rRNA gene-based microbial ecology techniques have revealed that the vast majority of bacterial species in nature have never been cultivated in a laboratory. Determining the ecological lifestyles of these bacteria is a major goal of microbial ecology. We combine modern cultivation techniques with DNA-based molecular methods to explore the metabolic potential of this uncultured diversity. The work focuses particularly on geothermal environments such as Dewar Creek in BC (photo), as these environments are known to be hotspots for exotic microbial diversity. For example, we recently isolated the first member of Candidate Division OP10, a phylum-level group previously known only from cultivation-independent surveys of 16S rRNA genes. We are presently studying the genomes of some of these uncultured bacteria using single-cell genomic methods. 2. Methane is both an extremely potent greenhouse gas and an inexpensive fuel. Our group studies methane-eating or methanotrophic bacteria in diverse natural and man-made environments. We have discovered a number of new methanotrophic species. Methanotrophs are useful for diverse biotechnologies, including: i) biofiltration to reduce atmospheric methane emissions from industrial point sources such as abandoned oil wells or oilsands tailings ponds; ii) co-oxidation of a diverse array of pollutants for bioremediation of oilsands tailings waste in situ; and iii) production of value-added products from natural gas with natural and engineered methanotroph strains.

近期论文

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Lee KC, Stott MB, Dunfield PF, Huttenhower C, McDonald IR, Morgan XC (2016) TheChthonomonas calidirosea genome is highly conserved across geography and distinct chemical and microbial environments in the Taupō Volcanic Zone, New Zealand. Appl Environ Microbiol. in press Aguilar M, Richardson E, Tan B, Walker G, Dunfield PF, Bass D, Nesbø C, Foght J, Dacks JB (2016) Next-generation sequencing assessment of eukaryotic diversity in oil sands tailings ponds sediments and surface water. J Eukaryotic Microbiol. in press Grasby SE, Fergusson G, Brady AL, Sharp CE, Dunfield PF, McMechan M (2016) Deep groundwater circulation and associated methane leakage in the Northern Canadian Rocky Mountains. Appl Geochem 68:10-18 Dedysh SN, Dunfield, PF (2016) Genus Methylocella in Whitman, WB (ed) Bergey’s Manual of Systematics of Archaea and Bacteria 3rd Edition, vol 1, Springer-Verlag, New York, NY Dunfield, PF (2016) Genus Methylohalobius in Whitman, WB (ed) Bergey’s Manual of Systematics of Archaea and Bacteria 3rd Edition, vol 1, Springer-Verlag, New York, NY Dedysh SN, Dunfield, PF (2016) Genus Methyloferula in Whitman, WB (ed) Bergey’s Manual of Systematics of Archaea and Bacteria 3rd Edition, vol 1, Springer-Verlag, New York, NY Flynn J, Hirayama H, Sakai Y, Dunfield PF, et al. (2016) Draft genomes of gammaproteobacterial methanotrophs isolated from marine ecosystems Genome Announce in press Eloe-Fadrosh EA, Paez-Espino D, Jarett J, Dunfield PF, Hedlund BP, Grasby SE, Brady AL, Dong H, Briggs BR, Li WJ, Goudeau D, Malmtrom R, Pati A, Rubin EM, Woyke T, Kyrpides NC, Ivanova NN (2016) Kryptonia, a novel bacterial phylum discovered through a global metagenomic survey. Nature Commun 7: Article 1047. Featured in: Hiding in Plain Sight, The Scientist, http://wwwthe-scientistcom/?articlesview/articleNo/42569/title/Hiding-in-Plain-Sight/ Lee KCY, Morgan XC, Power JF, Dunfield PF, Huttenhower C, Stott MB (2015)Complete genome sequence of the thermophilic Acidobacteria, Pyrinomonas methylaliphatogenes type strain K22T. Stand Genom Sci, 10: Article 101 Brady AL, Sharp CE, Grasby SE, Dunfield PF (2015) Anaerobic carboxydotrophic bacteria in Canadian geothermal springs identified using stable isotope probing.Front Microbiol 6: Article 897 Sharp CE, Smirnova AV, Kalyuzhnaya M, Bringel F, et al. (2015) Draft genome sequence of the moderately halophilic methanotroph, Methylohalobius crimeensis strain 10Ki.Genome Announce 3:Paper e00644-15 Wang X, Sharp CE, Jones GM, Brady AL, Grasby SE, Dunfield PF (2015) Stable-isotope-probing detects uncultured Planctomycetes as primary degraders of a complex heteropolysaccharide in soil. Appl Environ Microbiol 81: 4607-4615 Hamilton R, Kits K, Ramonovskay V, Rozova O, et al. (2015) Draft genomes of gammaproteobacterial methanotrophs Isolated from terrestrial ecosystems.Genome Announce 3: Paper e00515-15 Dedysh SN, Naumoff D, Vorobev A, Kyrpides N, et al. (2015) Draft genome sequence ofMethyloferula stellata AR4, an obligate methanotroph possessing only a soluble methane monooxygenase. Genome Announce 3:e01555-14 Kollah B, Dubey G, Dunfield PF, Mohanty SR (2015) Bioenergy crop Jatropha curcasamendment influences soil biogeochemistry in a tropical vertisol. Mitig Adapt Strat Glob Change 20:1459-1470 Dedysh SN, Dunfield PF (2014) Cultivation of methanotrophs in McGenity, T, Timmis, K, and Nogales, N Springer Protocols Handbooks Springer-Verlag Berlin Heidelberg (wwwspringerprotocolscom) Online resource doi: 101007/8623_2014_14 Sharp CE, Martinez A, Grasby SE, Dunfield, PF (2014) Methanotrophic bacteria in warm geothermal springs identified using stable isotope probing. FEMS Microbiol Ecol90:92-102 Sinninghe Damsté JS, Rijpstra WIC, Hopmans EC, Foesel BU, Wüst PK, Overmann J, Tank M, Bryant DA, Dunfield PF, Stott MB (2014) Ether- and ester-bound iso-diabolic acid and other lipids in members of Acidobacteria subdivision 4. Appl Environ Microbiol 80:5207-5218 Dunfield PF, Dedysh SN (2014) Methylocella: A gourmand among methanotrophs (Invited spotlight article). Trends Microbiol 22:368-369 Sharp CE, Smirnova AV, Graham JM, Stott MB, Khadka R, Moore TR, Grasby SE, Strack M, Dunfield PF (2014) Distribution and diversity of verrucomicrobial methanotrophs in geothermal and acidic environments. Environ Microbiol 16:1867-1878