Cheesman, Myles - University of East Anglia

个人简介

Myles Cheesman graduated from University of Cambridge with a B.A. in Natural Sciences in 1983. He then moved to UEA to join the group of Professor Andrew Thomson and studied for a PhD on the spectroscopic properties of nickel ions and iron-sulphur clusters in bacterial proteins. Post-doctoral research on heme-copper oxidases was followed by a period as Senior Research Officer responsible for the Magnetic Circular Dichroism (MCD) and Electron Paramagnetic Resonance (EPR) laboratories of the Centre for Metalloprotein Spectroscopy and Biology (CMSB) and then appointment to faculty in 2004. Myles’ research centres on the use of of MCD and EPR spectroscopy to study proteins containing transition metal ions: One third of all proteins would not function were it not for the presence of one or more metal cofactors. Enzymes which catalyse the redox transformation of small inorganic substrates, such as O2, NO, NO2- and S2O32-, invariably contain transition metal ions, both as electron transfer centres and at the active site itself. Thus the bacterial enzymes which drive the global nitrogen and sulphur cycles and the enzymes of the O2-terminated respiratory chains of many organisms are almost all transition metalloproteins. The techniques of MCD and EPR detect species which possess colour and paramagnetism and are therefore eminently suited to the study of these protein-bound inorganic complexes, providing information on metal identity, redox state, nature of ligands and interactions with other adjacent metal sites. These methods are currently used to study a wide variety of metalloproteins with a current emphasis on multiheme enzymes. Much of this work necessitates active collaboration with other research groups within the Henry Wellcome Laboratories for Biological Chemistry and with members of the School of Biological Sciences through the Centre for Metalloprotein Spectroscopy and Biology (CMSB).

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Girvan, H. M., Bradley, J. M., Cheesman, M. R., Kincaid, J. R., Liu, Y., Czarnecki, K., Fisher, K., Leys, D., Rigby, S. E. J., Munro, A. W.(2016)Analysis of Heme Iron Coordination in DGCR8: The Heme-Binding Component of the Microprocessor Complexin Biochemistry55.pp. 5073-5083 Full Text UEA Repository(Article) Lockwood, C., Burlat, B., Cheesman, M., Kern, M., Simon, J., Clarke, T., Richardson, D., Butt, J.(2015)Resolution of Key Roles for the Distal Pocket Histidine in Cytochrome c Nitrite Reductasesin Journal of the American Chemical Society137.pp. 3059–3068 Full Text UEA Repository(Article) Doyle, R., Marritt, S., Gwyer, J., Lowe, T., Cheesman, M., Butt, J., Tikhonova, T., Popov, V.(2013)Contrasting catalytic profiles of multiheme nitrite reductases containing CxxCK heme-binding motifsin JBIC Journal of Biological Inorganic Chemistry18.pp. 655-667 Full Text UEA Repository(Article) Van Wonderen, J. H., Oganesyan, V. S., Watmough, N. J., Richardson, D. J., Thomson, A. J., Cheesman, M. R.(2013)Unexpected weak magnetic exchange coupling between haem and non-haem iron in the catalytic site of nitric oxide reductase (NorBC) from Paracoccus denitrificans.in Biochemical Journal451.pp. 389-394 Full Text UEA Repository(Article) Bradley, J., Marritt, S., Kihlken, M., Haynes, K., Hemmings, A., Berks, B., Cheesman, M., Butt, J.(2012)Redox and Chemical Activities of the Hemes in the Sulfur Oxidation Pathway Enzyme SoxAXin Journal of Biological Chemistry287.pp. 40350-40359 Full Text UEA Repository(Article) Marritt, S., Lowe, T., Bye, J., McMillan, D., Shi, L., Fredrickson, J., Zachara, J., Richardson, D., Cheesman, M., Jeuken, L., Butt, J.(2012)A functional description of CymA, an electron-transfer hub supporting anaerobic respiratory flexibility in Shewanellain Biochemical Journal444.pp. 465-474 Full Text UEA Repository(Article) Bradley, J., Butt, J., Cheesman, M.(2011)Electrochemical titrations and reaction time courses monitored in situ by magnetic circular dichroism spectroscopyin Analytical Biochemistry419.pp. 110-116 Full Text UEA Repository(Article) Bradley, J., Silkstone, G., Wilson, M., Cheesman, M., Butt, J.(2011)Probing a Complex of Cytochromecand Cardiolipin by Magnetic Circular Dichroism Spectroscopy: Implications for the Initial Events in Apoptosisin Journal of the American Chemical Society133.pp. 19676-19679 Full Text UEA Repository(Article) White, G., Singleton, C., Todd, J., Cheesman, M., Johnston, A., Le Brun, N.(2011)Heme binding to the second, lower-affinity site of the global iron regulator Irr from Rhizobium leguminosarum promotes oligomerizationin FEBS Journal278.pp. 2011-2021 Full Text UEA Repository(Article) Driscoll, M. D., McLean, K. J., Cheesman, M. R., Jowitt, T. A., Howard, M., Carroll, P., Parish, T., Munro, A. W.(2011)Expression and characterization of Mycobacterium tuberculosis CYP144: Common themes and lessons learned in the M. tuberculosis P450 enzyme familyin Biochimica et Biophysica Acta - Proteins and Proteomics1814.pp. 76-87 Full Text UEA Repository(Article) Kemp, G., Clarke, T., Marritt, S., Lockwood, C., Poock, S., Hemmings, A., Richardson, D., Cheesman, M., Butt, J.(2010)Kinetic and thermodynamic resolution of the interactions between sulfite and the pentahaem cytochrome NrfA fromEscherichia coliin Biochemical Journal431.pp. 73-80 Full Text UEA Repository(Article) Singleton, C., White, G., Todd, J., Marritt, S., Cheesman, M., Johnston, A., Le Brun, N.(2010)Heme-responsive DNA binding by the global iron regulator Irr from Rhizobium leguminosarumin The Journal of Biological Chemistry285.pp. 16023-16031 Full Text UEA Repository(Article) Girvan, H. M., Levy, C. W., Williams, P., Fisher, K., Cheesman, M. R., Rigby, S. E. J., Leys, D., Munro, A. W.(2010)Glutamate-haem ester bond formation is disfavoured in flavocytochrome P450 BM3: characterization of glutamate substitution mutants at the haem site of P450 BM3in Biochemical Journal427.pp. 455-466 Full Text UEA Repository(Article) Macmillan, F., Van Wonderen, J., Cheesman, M., Van Der Hocht, I., Angerer, H., Gennis, R., Michel, H.(2010)Using EPR up close and from afar: Elucidating mechanisms in haem copper oxidasesin Biochimica et Biophysica Acta - Bioenergetics1797.pp. 97 Full Text UEA Repository(Article) Jervis, A., Crack, J., White, G., Artymiuk, P., Cheesman, M., Thomson, A., Le Brun, N., Green, J.(2009)The O2 sensitivity of the transcription factor FNR is controlled by Ser24 modulating the kinetics of [4Fe-4S] to [2Fe-2S] conversionin Proceedings of the National Academy of Sciences of the United States of America106.pp. 4659-4664 Full Text UEA Repository(Article) Bew, S., Cheesman, M., Sharma, S.(2009)Ferrocenium Salts Mediate para-tert-Butylcalixarene Synthesis.in ChemInform40. Full Text UEA Repository(Article) Girvan, H. M., Toogood, H. S., Littleford, R. E., Seward, H. E., Smith, W. E., Ekanem, I. S., Leys, D., Cheesman, M. R., Munro, A. W.(2009)Novel haem co-ordination variants of flavocytochrome P450 BM3in Biochemical Journal417.pp. 65-76 Full Text UEA Repository(Article) Kemp, G., Marritt, S., Xiaoe, L., Durrant, J., Cheesman, M., Butt, J.(2009)Opportunities for mesoporous nanocrystalline SnO2 electrodes in kinetic and catalytic analyses of redox proteinsin Biochemical Society Transactions37.pp. 368-372 Full Text UEA Repository(Article) McLean, K. J., Lafite, P., Levy, C., Cheesman, M. R., Mast, N., Pikuleva, I. A., Leys, D., Munro, A. W.(2009)The Structure of Mycobacterium tuberculosis CYP125: Molecular basis for cholesterol binding in a P450 needed for host infectionin Journal of Biological Chemistry284.pp. 35524-35533 Full Text UEA Repository(Article) Bew, S., Cheesman, M., Sharma, S.(2008)Ferrocenium salts mediate para-tert-butylcalixarene synthesisin Chemical Communicationspp. 5731-5733 Full Text UEA Repository(Article)