Blumenschein, Tharin - University of East Anglia

个人简介

Tharin obtained a BSc in Molecular Sciences and a PhD in Biochemistry from the University of São Paulo (Brazil). Her interests in Structural Biology then took her to Canada, where she used nuclear magnetic resonance (NMR) spectroscopy to study protein structure and dynamics in the regulation of muscle contraction as a postdoctoral fellow in Prof. Brian Sykes’s laboratory, in the University of Alberta, before moving to the UK to work with Prof. Steve Matthews at Imperial College London, where she studied proteins involved in cell invasion by the parasite Toxoplasma gondii. Research interests in the Blumenschein group involve using NMR spectroscopy to investigate structural and dynamic properties of proteins, and their role in protein interactions and function. Current projects include Chlamydia effectors, cell signalling in cancer, and the cytochrome c, as well as the study of intrinsically disordered proteins by NMR spectroscopy.

研究领域

Research interests in the Blumenschein group revolve around understanding protein structures and dynamics, and their role in protein interactions and function. We perform protein expression and purification to obtain isotopically labelled samples for nuclear magnetic resonance (NMR) spectroscopy studies, complemented by other biophysical techniques as necessary. Current research projects include the study of pathogen effectors, cell signalling in cancer and apoptosis, with a particular interest in intrinsically disordered proteins in these areas. Effectors are proteins produced by pathogens that contribute to the process of infection, both of animal and plant hosts. We are currently studying the structure and dynamics of Tarp, an effector from Chlamydia trachomatis involved in the cell invasion in human infection (in collaboration with Dr Ted Hackstadt, NIH, USA). Cell signalling is intrinsic to the process of cancer. The process of cell division and growth is usually tightly regulated in each cell, involving a number of proteins that interact with each other to maintain this balanced network of signals. We are looking at three different signalling processes that are involved in cancer and apoptosis: ADAM-15 interactions with different intracellular kinases (in collaboration with Prof Dylan Edwards), the E3 ligase WWP2 interactions with its targets (in collaboration with Dr Andrew Chantry), and the role of cytochrome c in apoptosis (in collaboration with Dr Jonathan Worral, Essex).

近期论文

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Karsisiotis, A. I., Deacon, O. M., Wilson, M. T., Macdonald, C., Blumenschein, T. M., Moore, G. R., Worrall, J. R.(2016)Increased dynamics in the 40-57 Ω-loop of the G41S variant of human cytochrome c promote its pro-apoptotic conformationin Scientific Reports6. Full Text UEA Repository(Article) Karsisiotis, A. I., Deacon, O. M., Rajagopal, B., Macdonald, C., Blumenschein, T. M. A., Moore, G., Worrall, J. R.(2015)Backbone resonance assignments of ferric human cytochrome c and the pro-apoptotic G41S mutant in the ferric and ferrous statesin Biomolecular NMR Assignments9.pp. 415-419 Full Text UEA Repository(Article) Boutemy, L., King, S., Win, J., Hughes, R., Clarke, T., Blumenschein, T., Kamoun, S., Banfield, M.(2011)Structures of Phytophthora RXLR Effector Proteins: a CONSERVED BUT ADAPTABLE FOLD UNDERPINS FUNCTIONAL DIVERSITYin Journal of Biological Chemistry286.pp. 35834-35842 Full Text UEA Repository(Article) Julien, O., Mercier, P., Allen, C. N., Fisette, O., Ramos, C. H. I., Lagüe, P., Blumenschein, T. M. A., Sykes, B. D.(2011)Is there nascent structure in the intrinsically disordered region of troponin I?in Proteins: Structure, Function, and Bioinformatics79.pp. 1240-1250 Full Text UEA Repository(Article) de Oliveira, J. F., Sforça, M. L., Blumenschein, T., Goldfeder, M. B., Guimarães, B. G., Oliveira, C. C., Zanchin, N. I., Zeri, A.(2010)Structure, Dynamics, and RNA Interaction Analysis of the Human SBDS Proteinin Journal of Molecular Biology396.pp. 1053-1069 Full Text UEA Repository(Article) Blumenschein, T.(2009)Biomolecular structures by solution magnetic nuclear resonanceWiley-VHC Verlag GmbH & Co KGaA, Weinheimpp. 963-987ISBN 978-3-527-40773-6 UEA Repository(Chapter)Blumenschein, T., Friedrich, N., Childs, R. A., Saouros, S., Carpenter, E. P., Campanero-Rhodes, M. A., Simpson, P., Chai, W., Koutroukides, T., Blackman, M. J., Feizi, T., Soldati-Favre, D., Matthews, S.(2007)Atomic resolution insight into host cell recognition by Toxoplasma gondiiin The EMBO Journal26.pp. 2808-2820 Full Text UEA Repository(Article) Saouros, S., Blumenschein, T., Sawmynaden, K., Marchant, J., Koutroukides, T., Liu, B., Simpson, P., Carpenter, E. P., Matthews, S. J.(2007)High-Level Bacterial Expression and Purification of Apicomplexan Micronemal Proteins for Structural Studiesin Protein and Peptide Letters14.pp. 411-415 Full Text UEA Repository(Article) Hoffman, R. M. B., Gagne, S. M., Blumenschein, T. M. A., Sykes, B. D.(2007)A functional role for the intrinsically disordered regions of troponin Iin Biophysical Journalpp. 396A UEA Repository(Article) Blumenschein, T. M. A., Carpenter, E. P., Saouros, S., Friedrich, N., Koutroukides, T., Soldati, D., Matthews, S.(2007)The cell-binding region of Toxoplasma gondii micronemal protein 1 presents a novel fold UEA Repository(Paper)Blumenschein, T., Stone, D., Fletterick, R., Mendelson, R., Sykes, B.(2006)Dynamics of the C-Terminal Region of TnI in the Troponin Complex in Solutionin Biophysical Journal90.pp. 2436-2444 Full Text UEA Repository(Article) Hoffman, R. M., Blumenschein, T., Sykes, B. D.(2006)An Interplay between Protein Disorder and Structure Confers the Ca2+ Regulation of Striated Musclein Journal of Molecular Biology361.pp. 625-633 Full Text UEA Repository(Article) Blumenschein, T., Stone, D., Fletterick, R., Mendelson, A., Sykes, B.(2005)Calcium-dependent Changes in the Flexibility of the Regulatory Domain of Troponin C in the Troponin Complexin The Journal of Biological Chemistry280.pp. 21924-21932 Full Text UEA Repository(Article) Blumenschein, T. M. A., Stone, D. B., Sykes, B. D.(2005)Dynamics of TnC in the skeletal troponin complexin Biophysical Journal88.pp. 359A UEA Repository(Article) Blumenschein, T., Gillis, T. E., Tibbits, G. F., Sykes, B. D.(2004)Effect of Temperature on the Structure of Trout Troponin Cin Biochemistry43.pp. 4955-4963 Full Text UEA Repository(Article) Blumenschein, T. M. A., Stone, D. B., Sykes, B. D.(2004)Troponin C in the skeletal ternary complexin Biophysical Journal86.pp. 215A UEA Repository(Article) Blumenschein, T. M. A., Gillis, T. E., Tibbits, G. F., Sykes, B. D.(2003)Effects of temperature on protein structure: Trout troponin Cin Biophysical Journal84.pp. 567A UEA Repository(Article) Gillis, T. E., Blumenschein, T., Sykes, B. D., Tibbits, G. F.(2003)Effect of Temperature and the F27W Mutation on the Ca2+Activated Structural Transition of Trout Cardiac Troponin Cin Biochemistry42.pp. 6418-6426 Full Text UEA Repository(Article) Blumenschein, T. M. A., Gillis, T. E., Tibbits, G. F., Sykes, B. D.(2002)Effect of temperature on trout cardiac troponin C structurein Biophysical Journal82.pp. 1887 UEA Repository(Article) Gillis, T. E., Blumenschein, T. M. A., Sykes, B. D., Tibbits, G. F.(2002)Site I binds Ca2+ in trout cardiac troponin Cin Biophysical Journal82.pp. 1886 UEA Repository(Article)