Malone, Jacob - University of East Anglia - School of Biological Sciences

个人简介

1997 - 2001 MBiochem, St-Catherine's College, Oxford University, UK 2001 - 2005 DPhil, Department of Plant Sciences, Oxford University, UK 2005 - 2011 Postdoctoral researcher, Biozentrum, University of Basel, CH 2011 - present Synergy Lecturer in Plant and Microbial Science, UEA/JIC, UK

研究领域

My group studies the molecular mechanisms underlying bacterial signal transduction during responses to the environment. To achieve this we employ a range of processes including genetics, cell and molecular microbiology and biochemistry. Our current research focuses on the control of rhizosphere colonisation by the widespread Gram-negative bacterium Pseudomonas fluorescens. Biocontrol agents; soil microorganisms that promote plant growth and/or suppress pathogens represent an attractive potential alternative to existing chemical pesticides. The commensal soil bacterium Pseudomonas fluorescens is a prominent biocontrol species that forms beneficial relationships with plants and suppresses fungal growth. The effectiveness of biocontrol is directly related to the effectiveness of bacterial rhizosphere colonisation. However, despite much research into biocontrol by P. fluorescens, the internal signalling systems within the bacteria that control root colonisation are only poorly understood. Recent work from our laboratory and others has identified an important role for the ubiquitous bacterial second messenger cyclic-di-GMP in the control of P. fluorescens root colonisation. Cyclic-di-GMP is found in almost every bacterial species on Earth, and is a critical component of the microbial decision-making machinery that controls when, where and how bacteria initiate biofilm formation, progress through the cell cycle, produce and secrete secondary metabolites or regulate motility and virulence factors. In my previous laboratory, we used in-vitro expression technology (IVET) to identify a range of P. fluorescens genes that are specifically up-regulated in the rhizosphere. These include potential cdG targets such as the wss exopolysaccharide synthase operon, as well as several cdG-related operons. We have since shown that these rhizosphere up-regulated cdG systems control important aspects of bacterial behaviour, including attachment to plant roots and swarming motility. At least one system appears to be crucial for the effective colonisation of the wheat rhizosphere. My group is currently working to functionally characterise these cdG systems, with the overall aim of constructing an integrated molecular model for cdG signalling during the process of P. fluorescens rhizosphere colonisation.

近期论文

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Little, R. H., Grenga, L., Saalbach, G., Howat, A. M., Pfeilmeier, S., Trampari, E., Malone, J. G.(2016)Adaptive remodeling of the bacterial proteome by specific ribosomal modification regulates Pseudomonas infection and niche colonisationin PLoS Genetics12. Full Text UEA Repository(Article) Pfeilmeier, S., Saur, I. M., Rathjen, J. P., Zipfel, C., Malone, J. G.(2016)High levels of cyclic-di-GMP in plant-associated Pseudomonas correlate with evasion of plant immunityin Molecular Plant Pathology17.pp. 521-531 Full Text UEA Repository(Article) Skotnicka, D., Smaldone, G. T., Petters, T., Trampari, E., Liang, J., Kaever, V., Malone, J. G., Singer, M., Søgaard-Andersen, L.(2016)A Minimal Threshold of c-di-GMP Is Essential for Fruiting Body Formation and Sporulation in Myxococcus xanthusin PLoS Genetics12. Full Text UEA Repository(Article) Pfeilmeier, S., Caly, D. L., Malone, J. G.(2016)Bacterial pathogenesis of plants: Future challenges from a microbial perspectivein Molecular Plant Pathology17.pp. 1298–1313 Full Text UEA Repository(Article) Mauchline, T., Chedom-Fotso, D., Chandra, G., Samuels, T., Greenaway, N., Backhaus, A., McMillan, V., Canning, G., Powers, S. J., Hammond-Kosack, K. E., Hirsch, P. R., Clark, I. M., Mehrabi, Z., Roworth, J., Burnell, J., Malone, J. G.(2015)An analysis of Pseudomonas genomic diversity in take-all infected wheat fields reveals the lasting impact of wheat cultivars on the soil microbiotain Environmental Microbiology17.pp. 4764-4778 Full Text UEA Repository(Article) Malone, J.(2015)Role of small colony variants in persistence of Pseudomonas aeruginosa infections in cystic fibrosis lungsin Infection and Drug Resistance2015.pp. 237-247 Full Text UEA Repository(Article) Trampari, E., Stevenson, C. E., Little, R. H., Wilhelm, T., Lawson, D. M., Malone, J. G.(2015)Bacterial rotary export ATPases are allosterically regulated by the nucleotide second messenger cyclic-di-GMPin Journal of Biological Chemistry290.pp. 24470-24483 Full Text UEA Repository(Article) Caly, D., Coulthurst, S., Geoghegan, J., Malone, J., Ryan, R.(2012)Socializing, networking and development: a report from the second ‘Young Microbiologists Symposium on Microbe Signalling, Organization and Pathogenesis’in Molecular Microbiology86.pp. 501-512 Full Text UEA Repository(Article) Malone, J., Jaeger, T., Manfredi, P., Dötsch, A., Blanka, A., Bos, R., Cornelis, G., Häussler, S., Jenal, U.(2012)The YfiBNR Signal Transduction Mechanism Reveals Novel Targets for the Evolution of Persistent Pseudomonas aeruginosa in Cystic Fibrosis Airwaysin PLoS Pathogens8. Full Text UEA Repository(Article) Malone, J., Jaeger, T., Spangler, C., Ritz, D., Spang, A., Arrieumerlou, C., Kaever, V., Landmann, R., Jenal, U.(2010)YfiBNR Mediates Cyclic di-GMP Dependent Small Colony Variant Formation and Persistence in Pseudomonas aeruginosain PLoS Pathogens6. Full Text UEA Repository(Article) Silby, M., Cerdeño-Tárraga, A., Vernikos, G., Giddens, S., Jackson, R., Preston, G., Zhang, X., Moon, C., Gehrig, S., Godfrey, S., Knight, C., Malone, J., Robinson, Z., Spiers, A., Harris, S., Challis, G., Yaxley, A., Harris, D., Seeger, K., Murphy, L., Rutter, S., Squares, R., Quail, M., Saunders, E., Mavromatis, K., Brettin, T., Bentley, S., Hothersall, J., Stephens, E., Thomas, C., Parkhill, J., Levy, S., Rainey, P., Thomson, N.(2009)Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescensin Genome Biology10.pp. R51 Full Text UEA Repository(Article)