Gross, Stephane - Aston University - School of Life & Health Sciences, Biology/Biosciences

个人简介

2000-2005 Postdoctoral Research Fellow, University of Medicine and Dentistry of New Jersey (USA) 2005-2007 Adjunct Assistant Professor, University of Medicine and Dentistry of New Jersey (USA) 2007-2009 Lecturer in Cellular and Molecular Biology, Liverpool John Moores University (UK) 2009 – Lecturer in Cellular Biology, Aston University

研究领域

The research in our laboratory aims to characterise the role, at the cellular level, of different actin binding/bundling proteins on the organisation of the actin cytoskeleton and the potential roles these proteins may have in disease progressions (such as cancer). Oncogenic transformation, metastasis, cellular aging and apoptosis are processes where cytoskeleton organization and protein synthesis are often synchronously deregulated. A factor that is essential for regulating both actin remodelling and ribosomal functions is the eukaryotic Elongation Factor 1A (eEF1A). Consequently, and maybe not surprisingly, increases in eEF1A levels have been observed to increase in oncogenic conditions and its level usually correlates to the degree of tumorgenesis. Understanding eEF1A’s non-canonical function towards actin regulation as well as the biological consequences of their interactions on the cytoskeleton and both localised and global protein translation could provide new avenues for therapy in such pathologies. Projects in the lab aim to further determine the importance of the eEF1A-actin interactions on the actin organization in both yeast (saccharomyces cerevisae) and mammalian systems as well as in vitro, using the state of the art facilities in cellular biology and molecular biology offered in our department. The S100A4 protein has also been linked to cancer progression and has been shown to enhance cellular migration, leading to a metastatic phenotype when it is over expressed in certain tumour cells. Our work and that of others has shown that S100A4 affect the actin cytoskeleton though interaction with the non muscle myosin network. Using cell culture and state of the art facilities in cellular biology and molecular biology, we aim to understand the molecular mechanisms that allow S100A4 to remodel the actin cytoskeleton and the consequences of such reorganisation on cell migration.

近期论文

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Hipkiss AR, Cartwright SP, Bromley C, Gross SR and Bill RM (2013)Carnosine: can understanding its actions on energy metabolism and protein homeostasis inform its therapeutic potential?Chem Cent J, in press Gross, S. R. (2013)Actin binding proteins: their ups and downs in metastatic life Cell adhesion and migration. 7, 2 Du, M. , Wang, G. , Ismail, T. M. , Gross, S.R. , Fernig, D. G. , Barraclough, R. & Rudland (2012) S100P dissociates myosin IIA filaments and focal adhesion sites to reduce cell adhesion and enhance cell migrationJournal of biological chemistry. 287, 19, p. 15330-15344 Bawa Z, Bland CE, Bonander N, Bora N, Cartwright SP, Conner MT, Darby RAJ, Dilworth MV, Holmes WJ, Jamshad M, Routledge SJ, Gross SR and Bill RM (2011)Understanding the yeast host cell response to recombinant membrane protein production Biochem Soc Trans. 2011 Jun;39(3):719-23. Goh, C. , Hersch, N. , Rudland, P. S. , Barraclough, R. , Hoffmann, B. & Gross, S. R. (2011)S100A4 downregulates filopodia formation through increased dynamic instability Cell adhesion and migration. 5, 5, p. 439-447 Wang Z, Collighan RJ, Gross SR, Danen EH, Orend G, Telci D, Griffin M.(2010) RGD-independent cell adhesion via a tissue transglutaminase-fibronectin matrix promotes fibronectin fibril deposition and requires syndecan-4/2 and {alpha}5{beta}1 integrin co-signaling. J Biol Chem. 17;285(51):40212-29Thamir, I, Zhang S., Fernig D., Gross S.R. , Martin-Fernandez M.L., Rudland P.S., See V., Tozawa K., Tynan C.J, Wang G., Wilkinson M.C and Barraclough R (2009). Self-association of calcium binding protein, S100A4 and metastasis.J. Biol. Chem., In press Gross S.R. and Kinzy T.G. (2007) Improper organization of the actin cytoskeleton affects protein synthesis at initiation.Mol. Cell. Biology. 27(5):1974-89 Anand M., Balar B., Ulloque R., Gross S. R. and Kinzy T.G. (2006). Domain and nucleotide dependence of the interaction between Saccharomyces cerevisiae translation Elongation Factors 3 and 1A.J. Biol. Chem.,281(43):32318-26 Chatterjee I., Gross S.R., Kinzy T.G. and Chen K.U.(2006).Rapid depletion of mutant eukaryotic initiation factor 5A at restrictive temperature reveals connections to actin cytoskeleton and cell cycle progression.Mol. Genet Genomics, 275(3):264-7 Gross S.R. and Kinzy T.G. (2005). The translation elongation factor 1A plays essential regulatory functions in the organization of both the actin cytoskeleton and cell morphology.Nature Structural and Molecular Biology, 12(9):772-8 Komar A.A., Gross S.R., Barth-Baus D., Strachan R., Hensold J.O., Kinzy T.G. and Merrick W.C. (2005). Novel characteristics of the biological properties of the yeast Saccharomyces cerevisiae eukaryotic initiation factor 2A.J. Biol. Chem., 280(16):15601–11 Anand M., Balar B.A., Gross S. R., Ortiz P.A., Ozturk S., Pittman Y.R., Ulloque R.,and Kinzy T.G. (2005).The Reactome: Translation Elongation. http://www.reactome.org/cgi-bin/frontpage Nur-E-Kamal A., Gross S.R., Pan Z., Balklava Z., Ma J. and Liu L.F. (2004)Nuclear Translocation of Cytochrome C during Apoptosis.J. Biol. Chem.:277(19), 24911-4 Gross S.R.*, Mercado M.L.T.*, Nur-E-Kamal A.*, Liu H.Y. *, Movahed R. and Meiners S. (2004).Neurite outgrowth by the alternatively spliced region of human tenascin-C is mediated b neuronal a7b1integrin. * Equally contributed to the workJ. Neurosciences. 24(1):238 –247 Gross S.R., Balklava Z. and Griffin M.(2003). The importance of tissue transglutaminase in the repair of extracellular matrices and cell death of dermal fibroblasts after exposure to a solarium UVA exposures.J. Invest. Dermat.,121(2):412-23 Gross S.R.*, Romero A.A.*,Cheng K.Y. Goldsmith N.K. and Geller H.M. (2003).An age-related increase in resistance to DNA damage-induced apoptotic cell death is associated with development of DNA repair mechanisms. * Equally contributed to the work.J. Neurochem., 84(6):1275-87. Balklava Z., Verderio E., Collingham R., Gross S.R., Adams J. and Griffin M. (2002). Analysis of tissue transglutaminase function in the migration of Swiss 3T fibroblasts: the active-state conformation of the enzyme does not affect cell motility but is important for its secretion. J. Biol. Chem., 277(19):16567-75. Verderio E., Gaudry C., Gross S.R., Smith C., Downes S. and Griffin M. (1999). Regulation of cell-surface tissue transglutaminase: effects on matrix storage of latent transforming growth factor-? binding protein-1. J. Histochem. Cytochem., 47:1417-1432 Verderio E, Nicholas B, Gross S.R., Griffin M.(1998). Regulated expression of tissue transglutaminase in Swiss 3T3 fibroblasts: effects on the processing of fibronectin, cell attachment and cell death.Exp. Cell Res. 239:119-138