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个人简介

教育经历 2005.9-2011.12, 中国科学院上海生命科学研究院,微生物学,博士 2003.9-2005.6, 河南大学, 生物工程, 工学学士 2000.9-2003.6, 河南牧业经济学院, 生物技术   工作经历 2016.9-至今, 中国科学院天津工业生物技术研究所,研究员 2013.10-2016.9, 德国马普煤炭研究所/菲利普马尔堡大学,博士后 2012.10-2013.9, 新加坡南洋理工大学,博士后研究员 2012.1-2012.9, 中国科学院上海生命科学研究院/上海工业生物技术研发中心,助理研究员/项目主管 2008.7-2011.12, 上海工业生物技术研发中心,项目负责人(兼) 2007.4-2008.6, 中国科学院上海生命科学研究院湖州工业生物技术中心,项目负责人(兼)

研究领域

工业生物技术是人类应对环境问题、能源问题和资源问题的重要解决方案,酶是核心发动机,广泛应用于食品,医药,农产品加工等行业,是实现生物催化合成的基本功能单元。团队围绕工业酶设计与绿色生物催化,重点开展酶分子工程、化酶催化工程、生物合成工程等方向的基础与应用研究。 主要研究进展和学术贡献: (1)针对人工酶设计筛选瓶颈,聚焦氨基酸残基精准定位和密码子设计,建立了精简密码子设计体系模型,突破了筛选瓶颈,揭示了密码子设计原理。 (2)针对酶催化机制,基于晶体结构解析与多尺度计算模拟,解析了底物特异识别与手性控制机制,为酶的理性设计奠定了基础。 (3)针对工业酶精准设计应用,将分子构象动力学、脯氨酸诱导设计等策略用于酶的理性设计,实现了工业酶的精准设计与人工途径创建,构建了系列高效生物催化剂。

近期论文

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Chen Q, Qu G, Li X, Feng M, Yang F, Li Y, Li J, Tong F, Song S, Wang Y*, Sun Z*, Luo G. Active and stable alcohol dehydrogenase-assembled hydrogels via synergistic bridging of triazoles and metal ions. Nat. Commun., 2023, 14, 2117. Reetz MT*, Sun Z*, Qu G*. Book review of “Enzyme engineering: Selective catalysts for applications in biotechnology, organic chemistry, and life science”. Green Syn. Catal., 2023, https://doi.org/10.1016/j.gresc.2023.02.006 曲戈*,孙周通*.催化混杂性驱动的酶功能重塑.生物技术通报,2023,39(2),https://doi.org/10.13560/j.cnki.biotech.bull.1985.2022-1305 王钦宏,张以恒,田朝光,孙周通,马延和.低碳生物合成:机遇与挑战。科学通报 (Chinese Sci. Bull.),2023,68,https://doi.org/10.1360/TB-2022-1194 Li Y, Bao C, Sun Z, Zhang W, Yuan B. Comparison between Chemoenzymatic and Bienzymatic Cascades Leading to Morita–Baylis–Hillman Adducts. ChemCatChem, 2023, e202201621. Huang W, Huang S, Sun Z, Zhang W, Zeng Z, Yuan B. Chemoenzymatic Synthesis of Sterically Hindered Biaryls by Suzuki Coupling and Vanadium Chloroperoxidase Catalyzed Halogenations. ChemBioChem, 2023, 24(1), e202200610. Li Jk#, Qu G#, Li X#, Tian Y, Cui C, Zhang F, Zhang W, Ma J*, Reetz MT*, Sun Z*. Rational enzyme design for enabling biocatalytic Baldwin cyclization and asymmetric synthesis of chiral heterocycles. Nat. Commun., 2022, 13:7813. 曲戈,袁波,孙周通*.工业蛋白质理性设计与应用. 生物工程学报, 2022, 38(11): 4068-4080. Chen Q, An Y, Feng M, Li J, Li Y, Tong F, Qu G, Sun Z, Wang Y, Luo G. An enzyme-assembled gel monolithic microreactor for continuous flow asymmetric synthesis of aryl alcohols. Green Chem., 2022, 24(24), 9508-9518. Zhang J, Li X, Chen R, Tan X, Liu X, Ma Y, Zhu F, An C, Wei G, Yao Y, Yang L, Zhang P, Wu Q, Sun Z, Wang B, Gao S, Cui C. Actinomycetes-derived imine reductases with a preference towards bulky amine substrates. Commun. Chem., 2022, 5(1), 123. Ge R, Zhang P, Dong X, Li Y, Sun Z, Zeng Y, Chen B, Zhang W. Photobiocatalytic Decarboxylation for the Synthesis of Fatty Epoxides from Renewable Fatty Acids. ChemSusChem. 2022, 15(20), e202201275. Wu X, Jiang Y, Wang Z, Yu X, Sun Z, Luo W. Enhanced thermostability of formate dehydrogenase via semi-rational design. Mol. Catal., 2022, 530, 112628. Yu ZP, An C, Yao Y, Wang CY, Sun Z, Cui C, Liu L, Gao S. A combined strategy for the overproduction of complex ergot alkaloid agroclavine. Synth. Syst. Biotechnol., 2022, 7(1), 1126-1132. Ming H, Yuan B, Qu G*, Sun Z*. Engineering the activity of Amine Dehydrogenase in the Asymmetric Reductive Amination of Hydroxyl Ketones. Catal. Sci. Technol., 2022, 12(19), 5952-5960. Jiang Y#, Li X#, Liu B, Tong F, Qu G*, Sun Z*. Engineering the hydrogen transfer pathway of an alcohol dehydrogenase to increase activity by rational enzyme design. Mol. Catal., 2022, 530, 112603. Wang P, Han X, Liu X*, Lin R, Chen Y, Sun Z*, Zhang W*. Synthesis of Enantioenriched Sulfoxides by an Oxidation-Reduction Enzymatic Cascade. Chem. Eur. J. 2022, 28(61), e202201997. Lu J, Wang Z, Jiang Y, Sun Z*, Luo W*. Modification of the substrate specificity of leucine dehydrogenase by site-directed mutagenesis based on biocomputing strategies. Syst. Microbiol. and Biomanuf., 2022. https://doi.org/10.1007/s43393-022-00116-5. Sang X, Tong F, Zeng Z, Wu M, Yuan B, Sun Z, Sheng X, Qu G, Alcalde M, Hollmann F, Zhang W. A Biocatalytic Platform for the Synthesis of Enantiopure Propargylic Alcohols and Amines. Org. Lett., 2022, 24(23), 4252-4257. Qin Z, Zhang X, Sang X, Zhang W, Qu G*, Sun Z*. Carboxylic acid reductases enable intramolecular lactamization reactions. Green Synth. Catal., 2022, 3(3), 294-297. Wang J#, Qu G#, Xie L, Gao C, Jiang Y, Zhang YPJ, Sun Z*, You C*. Engineering of a thermophilic dihydroxy-acid dehydratase toward glycerate dehydration for in vitro biosystems. Appl. Microbiol. Biotechnol., 2022, 106, 3625-3637. Li Y, Zhang P, Sun Z, Li H, Ge R, Sheng X, Zhang W. Peroxygenase-Catalyzed Selective Synthesis of Calcitriol Starting from Alfacalcidol. Antioxidants, 2022, 11, 1044. Zhang J#, Liao D#, Chen R#, Zhu F, Ma Y, Gao L, Qu G, Cui C, Sun Z*, Lei X*, Gao S*. Tuning an Imine Reductase for the Asymmetric Synthesis of Azacycloalkylamines by Concise Structure-Guided Engineering. Angew. Chem. Int. Ed., 2022. 61(24), e202201908. Song S#, Jiang Y#, Chen R, Su W, Liang W, Yang D, Li J, Zhang W, Gao S, Yuan B*, Qu G*, Sun Z*. Whole-cell Biotransformation of Penicillin G by a Three-enzyme Co-expression System with Engineered Deacetoxycephalosporin C Synthase. ChemBioChem, 2022, 23(11), e202200179. Yang D, Su W, Jiang Y, Gao S, Li X, Qu G*, Sun Z*. Biosynthesis of β-lactam nuclei in yeast. Metab. Eng., 2022, 72, 56-65. 毕悦欣,蒋迎迎,覃宗敏,曲戈*,孙周通*.醇脱氢酶金属离子绑定位点的可替换性. 生物工程学报, 2022, 38(04),1518-1526. Jiang Y#, Qu G#, Sheng X, Tong F, Sun Z*. Unraveling the mechanism of enantio-controlling switches of an alcohol dehydrogenase toward sterically small ketone. Catal. Sci. Technol., 2022, 12, 1777-1787. Qu G, Bi Y, Liu B, Li JK, Han X, Liu W, Jiang Y, Qin Z, Sun Z*. Unlocking the Stereoselectivity and Substrate Acceptance of Enzymes: Proline Induced Loop Engineering Test. Angew. Chem. Int. Ed., 2022, 61 (1), e202110793. Bi H#, Qu G#, Wang S, Zhuang Y, Sun Z*, Liu T*, Ma Y. Biosynthesis of a Rosavin Natural Product in Escherichia coli by Glycosyltransferase Rational Design and Artificial Pathway Construction. Metab. Eng., 2022, 69, 15-25. Dong C, Qu G, Guo J, Wei F, Gao S, Sun Z, Jin L, Sun X, Rochaix JD, Miao Y, Wang R. Rational design of geranylgeranyl diphosphate synthase enhances carotenoid production and improves photosynthetic efficiency in Nicotiana tabacum. Science Bulletin, 2022, 67(3), 315-327. Tong F#, Qin Z#, Wang H#, Jiang Y, Li J, Ming H, Qu G, Xiao Y*, Sun Z*. Biosynthesis of Chiral Amino Alcohols via an Engineered Amine Dehydrogenase in E. coli. Front. Bioeng. Biotechnol., 2021, 9: 778584. Wu R, Yu Y, Wang Y, Wang YZ, Song H, Ma C, Qu G, You C, Sun Z, Zhang W, Li A, Li CM, Yong YC, Zhu Z. Wastewater-powered high-value chemical synthesis in a hybrid bioelectrochemical system. iScience, 2021, 103401. 张发光, 曲戈, 孙周通*, 马军安*. 从化学合成到生物合成—天然产物全合成新趋势. 合成生物学, 2021, 2(5), 674-696. 张武元, 袁波, 曲戈, 孙周通. 光促酶催化反应设计及生物合成应用.生物学杂志 (J. Biol.), 2021, 38(5), 1-11. 特约综述 Li Y, Yuan B, Sun Z, Zhang W. C–H bond functionalization reactions enabled by photobiocatalytic cascades. Green Synth. Catal., 2021, 2 (3), 267-274. Chen Q, Guo M, Bi Y, Qu G, Sun Z, Wang Y, Luo G. Whole-cell biocatalytic synthesis of S-(4-chlorophenyl)-(pyridin-2-yl) methanol in a liquid–liquid biphasic microreaction system. Bioresource Technol., 2021, 330: 125022. Li J#, Qu G#, Shang N, Chen P, Men Y, Liu W, Mei Z, Sun Y*, Sun Z*. Near-perfect control of the regioselective glucosylation enabled by rational design of glycosyltransferases. Green Synth. Catal., 2021, 2, 45-53. Acevedo-Rocha, CG*, Hollmann F*, Sanchis J*, Sun Z*. A Pioneering Career in Catalysis: Manfred T. Reetz. ACS Catal., 2020, 10, 15123-15139. (invited) Wang H#, Qu G#, Li JK#, Ma JA, Guo J, Miao Y, Sun Z*. Data mining of amine dehydrogenases for the synthesis of enantiopure amino alcohols. Catal. Sci. Technol., 2020. 10, 5945-5952. 蒋迎迎,曲戈,孙周通*. 机器学习助力酶定向进化. 生物学杂志 (J. Biol.), 2020, 37(4), 1-11. 特约综述 Mei Z#, Zhang K#, Qu G, Li JK, Liu B, Ma JA, Tu R*, Sun Z*. A High-Throughput Fluorescence Assay for Ketone Detection and its Applications in Enzyme Mining and Protein Engineering. ACS Omega, 2020, 5, 13588-13594. Qu G#, Li A#, Acevedo-Rocha CG#, Sun Z*, Reetz MT*. The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes. Angew. Chem. Int. Ed., 2020, 59, 13204-13231. (invited) Chen J#, Fan F#, Qu G#, Tang J, Xi Y, Bi C, Sun Z*, Zhang X*. Identification of Absidia orchidis steroid 11β-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone. Metab. Eng., 2020, 57, 31-42.

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