周佳海 - 中国科学院深圳先进技术研究院 -

个人简介

Professional experience 2020-now Professor, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences 2006-2020 Professor, State Key Laboratory of Bio-organic Chemistry and Natural Products Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences 2000-2001 Assistant Professor, State Key Laboratory of Bio-organic Chemistry and Natural Products Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences 1993-1995 Research Assistant, Nanjing Chemical Plant Education 2001-2006 Postdoctoral Research Fellow, University of Michigan 1995-2000 Ph.D., Bioorganic Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences 1989-1993 B.S., Analytical Chemistry, Nanjing University

研究领域

In-Silico Enzyme Engineering In silico design (ISD) methods are used to discover new biocatalysts and improve existing enzyme performance. Deep learning methods have been increasingly applied to find patterns in data favorable to predicting substrate specificity, and guiding rational design to improve enzyme stability, solubility, and other characteristics. High Throughput Mutant Screening for Repurposed Enzyme High throughput screening or selection methods plays an extremely significant role in successful evolutionary enzyme engineering, which greatly increases the chance of obtaining desired properties and reduce the time and cost. By taking advantage of automation, high throughput screening methods can streamline traditional screening process. Most importantly, designed methods can make the desired mutants easy to detect.

近期论文

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Zhi-Pu Li, Lanteng Wang, Yan Liu, Xiao-Qiong Pei, M. Qaiser Fatmi, Zhuanglin Shen, Jian Zhao, Hui Lin, Jiahai Zhou, Zhong-Liu Wu, Angew. Chem. Int. Ed. 2025, e202423117. Su n, Z.; Zang, X.; Zhou, Q.; Ohashi, M.; Houk, K. N.#; Zhou, J.#; Tang, Y.# Iminium Catalysis in Natural Diels-Alderase. Nat. Catal. 2025. https://doi.org/10.1038/s41929-025-01294-w. Jia-Jing Li, Xiao-Jian Zhou, Juan Ao, Jin-Tao Gao, An-Ni Wang, Zhuang-Lin Shen, Yang Gu, Jia-Hai Zhou, Yong-Zheng Chen* . (2024) . P450DA Monooxygenase-Catalyzed Chemoselective and Enantiodivergent Epoxidation of Unactivated Alkenes. ACS Catalysis . 14, (21), 16175–16183. Xin Zang, Undramaa Bat-Erdene, Weixue Huang, Zhongshuo Wu, Steve E Jacobsen, Yi Tang#, Jiahai Zhou#. (2024) Structural bases of dihydroxy acid dehydratase inhibition and biodesign for self-resistance. BioDesign Research. Hou X, Feng J, Franklin JL, Russo R, Guo Z, Zhou J#, Gao JM#, Liu HW#, Wang B#. (2024) Mechanistic Insights from the Crystal Structure and Computational Analysis of the Radical SAM Deaminase DesII. Adv Sci. 28: e2403494. Qian Tang, Yuxin Huang, Zhuanglin Shen, Linhui Sun, Yang Gu, Huiqing He, Yanhong Chen, Jiahai Zhou, Limin Zhang, Cuihuan Zhao, Shisong Ma, Yunhai Li, Jie Wu#, Qiao Zhao# (2024) 6-Phosphogluconate dehydrogenase 2 bridges the OPP and shikimate pathways to enhance aromatic amino acid production in plants. SCIENCE CHINA Life Sciences‍(Online ahead of print) Jie Wu, Yanhong Chen, Yuxin Huang, Bingbing Hao, Shengkun Dai, Lingling Zhao, Zhehui Zhao, Cuihuan Zhao, Limin Zhang, Yunhai Li, Xuexia Xu, Nan Li, Ancheng C. Huang, Jiahai Zhou, Minjia Tan, Wentao Zhu#, Qiao Zhao# (2024) Cytosolic aminotransferase VAS1 coordinates aromatic amino acids biosynthesis and metabolism. Sci Adv 10(2): eadk0738. Dhanaraju Mandalapu, Xinjian Ji, Jinfeng Chen, Chuchu Guo, Wan-Qiu Liu, Wei Ding, Jiahai Zhou, Qi Zhang#. (2018) Thioesterase-Mediated Synthesis of Teixobactin Analogues: Mechanism and Substrate Specificity. J Org Chem. 83 (13): 7271-7275. Wan, Zheng, Man Shi, Yanqing Gong, Massimo Lucci, Jinjin Li, Jiahai Zhou, Xiao-Liang Yang#, Moreno Lelli#, Xiao He#, and Jiafei Mao#. (2024) Multitasking Pharmacophores Support Cabotegravir-Based Long-Acting HIV Pre-Exposure Prophylaxis (PrEP). Molecules, 29 (2): 376. Tzu-Yu Chen, Jinfeng Chen, Mark W. Ruszczycky, Dalton Hilovsky, Tyler Hostetler, Xiaojing Liu#, Jiahai Zhou#, and Wei-chen Chang# (2024). Variation in Biosynthesis and Metal-Binding Properties of Isonitrile-Containing Peptides Produced by Mycobacteria versus Streptomyces. Acs Catal, 14, 4975–4983. Jie Zheng, Zhuanglin Shen, Jin-Ming Gao #, Jiahai Zhou #, and Yang Gu #. (2023） Enzymatic Photodecarboxylation on Secondary and Tertiary Carboxylic Acids . Org. Lett. https://doi.org/10.1021/acs.orglett.3c03356 Xiaolin Zou, Zhen Hui, Robert A. Shepherd, Shuaiqiang Zhao, Yanfei Wu, Zhuanglin Shen, Cuiping Pang, Shipeng Zhou, Zehai Yu, Jiahai Zhou, Bradly S. Moore, Laura M. Sanchez, Xiaoyu Tang #.（2023）Unveiling a CAAX Protease-Like Protein Involved in Didemnin Drug Maturation and Secretion. Adv. Sci. 2023, 2306044. Jingkun Shi, Xin Zang, Zhijie Zhao, Zhuanglin Shen, Wei Li, Guiyun Zhao, Jiahai Zhou#, and Yi-Ling Du*. 2023 Conserved Enzymatic Cascade for Bacterial Azoxy Biosynthesis . J. Am. Chem. Soc . https://doi.org/10.1021/jacs.3c12018 Yuanyuan Huang, Yanfei Wu, Han Hu, Bangzhuo Tong, Jie Wang, Siyu Zhang, Yanyi Wang, Jicong Zhang, Yue Yin, Shengkun Dai, Wenjuan Zhao, Bolin An, Jiahua Pu, Yaomin Wang, Chao Peng, Nan Li, Jiahai Zhou#, Yan Tan #, Chao Zhong#. (2023) Accelerating the design of pili-enabled living materials using an integrative technological workflow. Nat Chem Biol. https://doi.org/10.1038/s41589-023-01489-x Wu L., Wang Z., Cen Y.#, Wang B.#, Zhou J.# (2023). Reply to Correspondence on “Structural Insight into the Catalytic Mechanism of the Endoperoxide Synthase FtmOx1”. Angew Chem Int Edit, 62, e202306059. Jun-Bin He, Lian Wu, Wanqing Wei, Song Meng, Zheng-Tao Liu, Xuan Wu, Hai-Xue Pan, Sheng Yang, Yong Liang#, Jiahai Zhou#, Gong-Li Tang#. (2023). Enzymatic catalysis favours eight-membered over five-membered ring closure in bicyclomycin biosynthesis. Nat Catal, 6, 637–648. Yang, Qin, Zhenkun Zhang, Lin Wang, Xiwen Xing#, Jiahai Zhou#, and Long Li#. (2023). Preparation and Characterization of Metal–Organic Framework Coatings for Improving Protein Crystallization Screening. Nanomaterials, 13(14): 2064. Dong-Dong Wu, Sheng Jin, Ruo-Xiao Cheng, Wen-Jie Cai#, Wen-Long Xue, Qing-Qing Zhang, Le-Jie Yang, Qi Zhu, Meng-Yao Li, Ge Lin, Yi-Zhen Wang, Xue-Pan Mu, Yu Wang, Igor Ying Zhang, Qi Zhang, Ying Chen, Sheng-Yang Cai, Bo Tan, Ye Li, Yun-Qian Chen, Pu-Juan Zhang, Chen Sun, Yue Yin, Ming-Jie Wang, Yi-Zhun Zhu, Bei-Bei Tao, Jia-Hai Zhou#, Wei-Xue Huang#, and Yi-Chun Zhu#. (2023). Hydrogen sulfide functions as a micro-modulator bound at the copper active site of Cu/Zn-SOD to regulate the catalytic activity of the enzyme. Cell Reports, 42, (7): 112750. Liu J., Lu J., Zhang C., Zhou Q., Jamieson C.S., Shang C., Houk K.N. #, Zhou J. #, Hu Y. #(2023). Tandem intermolecular [4 + 2] cycloadditions are catalysed by glycosylated enzymes for natural product biosynthesis. Nat. Chem, 15, 1083–1090. Xu X.#, Yang Q., Wang L., Zheng J., Gu Y., Xing X.#, Zhou J.# (2023) Enzymatic hydrolysis on L-azetidine-2-carboxylate ring opening. Catal Sci & Technol, 13: 3953-3962. Ohashi, M., D. Tan, J. Lu, C. S. Jamieson, D. Kanayama, J. Zhou#, K. N. Houk# and Y. Tang# (2023) Enzymatic cis-Decalin Formation in Natural Product Biosynthesis. J Am Chem Soc, 145(6): 3301-3305. Xu, X.#, L. Yang, X. Zhang, X. Xing, J. Zhou# (2023) Characterization and structural basis of D-cysteine desulfhydrase from Pectobacterium atrosepticum. Tetrahedron, 130:133174. Wu P., Y. Gu, L. Liao, Y. Wu, J. Jin, Z. Wang, J. Zhou, S. Shaik#, B. Wang# (2022) Coordination Switch Drives Selective C−S Bond Formation by the Non-heme Sulfoxide Synthases. Angew Chem Int Edit, 61(50): e202214235. Wang L., X. Zang, J. Zhou# (2022) Synthetic biology: A powerful booster for future agriculture. Advanced Agarochem ,1(1): 7-11. Zhang, J., X. Hou, Z. Chen, Y. Ko, M. W. Ruszczycky, Y. Chen, J. Zhou# and H. W. Liu# (2022) Dioxane bridge formation during the biosynthesis of spectinomycin involves a twitch radical SAM dehydrogenase that may have evolved from an epimerase. J Am Chem Soc, 144(22): 9910-9919. Huang, X., J. Feng, J. Cui, G. Jiang, W. Harrison, X. Zang, J. Zhou, B. Wang# and H. Zhao# (2022) Photoinduced chemomimetic biocatalysis for enantioselective intermolecular radical conjugate addition. Nat Catal, 5: 586–593. Zhang, X., Z. Xu, J. Zhou, X. Xing# and L. Li# (2022) Enhancement of protein crystallization using nano-sized metal–organic framework. Crystals, 12(5): 578. Lee, Y. H., X. Hou, R. Chen, J. Feng, X. Liu, M. W. Ruszczycky, J. M. Gao#, B. Wang#, J. Zhou# and H. W. Liu# (2022) Radical S-Adenosyl methionine enzyme BlsE catalyzes a radical-mediated 1,2-diol dehydration during the biosynthesis of Blasticidin S. J Am Chem Soc, 144(10): 4478-4486. Chen, T., Z. Zheng, X. Zhang, J. Chen, L. Cha, Y. Tang, Y. Guo#, J. Zhou#, B. Wang#, H. Liu# and W. Chang# (2022) Deciphering the reaction pathway of mononuclear iron enzyme-catalyzed N≡C triple bond formation in isocyanide lipopeptide and polyketide biosynthesis. Acs Catal, 12(4): 2270-2279. Wu, L., Z. Wang, Y. Cen#, B. Wang# and J. Zhou# (2022) Structural insight into the catalytic mechanism of the endoperoxide synthase FtmOx1. Angew Chem Int Ed Engl, 61(12): e202112063. Cheng, Q., Y. Cheung, C. Liu, Q. Xiao, B. Sun, J. Zhou, E. W. C. Chan, R. Zhang and S. Chen (2021) Structural and mechanistic basis of the high catalytic activity of monooxygenase Tet(X4) on tigecycline. BMC Biol, 19(1): 262. Wen, W., Y. Zhang, Y. Zhang, Q. Yu, C. Jiang, M. Tang, J. Pu, L. Wu, Y. Zhao, T. Shi, J. Zhou# and G. Tang# (2021) Reductive inactivation of the hemiaminal pharmacophore for resistance against tetrahydroisoquinoline antibiotics. Nat Commun, 12(1): 7085. Li, S., P. D. Bury, F. Huang, J. Guo, G. Sun, A. Reva, C. Huang, X. Jian, Y. Li, J. Zhou, Z. Deng, F. J. Leeper#, P. F. Leadlay#, M. V. B. Dias# and Y. Sun# (2021) Mechanistic insights into dideoxygenation in gentamicin biosynthesis. Acs Catal, 11(19): 12274-12283. Xie, L., X. Zang, W. Cheng, Z. Zhang, J. Zhou#, M. Chen# and Y. Tang# (2021) Harzianic acid from Trichoderma afroharzianum is a natural product inhibitor of acetohydroxyacid synthase. J Am Chem Soc, 143(25): 9575-9584. Xiang, M. Wang, Wu, Z. Lu, J. Tang, J. Zhou, W. Huang#, and G. Zhang# (2021) Structural insights into xylanase mutant 254RL1 for improved activity and lower pH optimum, Enzyme Microb Technol, 147: 109786. Liu, N., L. Wu, J. Feng, X. Sheng#, J. Li, X. Chen, J. Li, W. Liu, J. Zhou#, Q. Wu#, and D. Zhu# (2021) Crystal structures and catalytic mechanism of L-erythro-3,5-diaminohexanoate dehydrogenase and rational engineering for asymmetric synthesis of β-amino acids, Angew Chem Int Ed, 60: 10203-10. Gao, D., C. S. Jamieson, G. Wang, Y. Yan, J. Zhou#, K. N. Houk#, and Y. Tang# (2021) A polyketide cyclase that forms medium-ring lactones. J Am Chem Soc, 143: 80-84. Chen, T., J. Chen, Y. Tang, J. Zhou#, Y. Guo# and W. Chang# (2021) Current understanding toward isonitrile group biosynthesis and mechanism. Chin J Chem, 39(2): 463-472. Yan, Y., X. Zang, C. S. Jamieson, H. Lin, K. N. Houk, J. Zhou# and Y. Tang# (2020). Biosynthesis of the fungal glyceraldehyde-3-phosphate dehydrogenase inhibitor heptelidic acid and mechanism of self-resistance. Chem Sci, 11(35): 9554-9562. Ohashi, M., C. S. Jamieson, Y. Cai, D. Tan, D. Kanayama, M. C. Tang, S. M. Anthony, J. V. Chari, J. S. Barber, E. Picazo, T. B. Kakule, S. Cao, N. K. Garg, J. Zhou#, K. N. Houk# and Y. Tang# (2020) An enzymatic Alder-ene reaction. Nature, 586(7827): 64-69. Chen, C., L. Su#, L. Wu, J. Zhou and J. Wu# (2020) Enhanced the catalytic efficiency and thermostability of maltooligosyltrehalose synthase from Arthrobacter ramosus by directed evolution. Biochem Eng J, 162(2020):107724. Cheng, R., L. Wu, R. Lai, C. Peng, N. Naowarojna, W. Hu, X. Li, S. A. Whelan, N. Lee, J. Lopez, C. Zhao, Y. Yong, J. Xue, X. Jiang, M. W. Grinstaff, Z. Deng, J. Chen, Q. Cui#, J. Zhou# and P. Liu# (2020) Single-step replacement of an unreactive C-H bond by a C-S bond using polysulfide as the direct sulfur source in anaerobic ergothioneine biosynthesis. ACS Catal, 10(16): 8981-8994. Dong, Q., S. Yuan, L. Wu, L. Su, Q. Zhao, J. Wu, W. Huang# and J. Zhou# (2020) Structure-guided engineering of a Thermobifida fusca cutinase for enhanced hydrolysis on natural polyester substrate. Bioresour and Bioprocess, 7(1). Yang, X., L. Wu, A. Li, L. Ye#, J. Zhou# and H. Yu# (2020) The engineering of decameric D-fructose-6-phosphate aldolase A by combinatorial modulation of inter- and intra-subunit interactions. Chem Commun (Camb), 56(55): 7561-7564. Li, A., C. G. Acevedo-Rocha, L. D'Amore, J. Chen, Y. Peng, M. Garcia-Borras, C. Gao, J. Zhu, H. Rickerby,S. Osuna#, J. Zhou# and M. T. Reetz# (2020) Regio- and stereoselective steroid hydroxylation at C7 by cytochrome P450 monooxygenase mutants. Angew Chem Int Ed Engl, 59(30): 12499-12505. Chen, T., J. Chen, Y. Tang, J. Zhou#, Y. Guo# and W. Chang# (2020) Pathway from N-alkylglycine to alkylisonitrile catalyzed by Iron (II) and 2-oxoglutarate-dependent oxygenases. Angew Chem Int Ed Engl, 59(19): 7367-7371.