个人简介
吴长艾,女,山东巨野人。2004年获得山东农业大学理学博士学位,至今在山东农业大学生命科学学院工作。生物化学与分子生物学国家级教学团队成员。承担本科生《生物化学A》、《基础生物化学B》和《基因工程》;研究生《基因工程原理和技术》等课程的教学工作。“泰山学者”特聘岗位学术骨干。主要从事重要经济作物棉花及模式植物拟南芥耐盐、抗旱等重要基因的克隆、鉴定,探讨其调控机制及在作物遗传改良上的应用价值。近年来,承担了转基因生物新品种培育科技重大专项、国家自然科学基金和山东省自然基金等课题6项,参加2项。获国家发明专利4项。在《PlantandcellPhysiology》、《RNA》、《FEBSJournal》、《PlantScience》和《作物学报》等国内外著名学术刊物上发表研究论文30余篇。
教学工作
承担本科生《基础生物化学》,《基础生物化学实验》;研究生《基因工程原理与技术》、《基因表达调控》等课程的教学工作
研究领域
研究方向
1.棉花和拟南芥盐碱、干旱等非生物逆境响应基因的分离、鉴定及分子调控
2.棉花和拟南芥盐碱、干旱等非生物逆境响应的脱落酸、茉莉酸等激素调控
科研项目
1.转基因生物新品种培育科技重大专项《棉花抗旱、耐盐和低温重要新基因的克隆和功能验证》2009-2012,主持
2.国家自然科学基金《棉花盐诱导的4个海藻糖6-磷酸合酶和6个LEA基因的生理功能及其应用研究》2011-2013,主持
3.国家自然科学基金《五个拟南芥盐和干旱诱导的miRNAs的功能鉴定及调控途径研究》2010-2012,主持
4.国家自然科学基金《水稻干旱、盐胁迫相关microRNAs的分离和表达调控分析》2006-2008,主持
5.教育部“长江学者创新团队发展计划”《作物高产优质的生理与分子基础》2007-2009,子课题主持
6.山东省自然科学基金《“棉花新型转录调节因子GhPLATZ1的功能和作用机理研究》,2015-2017,主持
7.山东农业大学博士基金《棉花Na+/H+逆向运转体基因的耐盐机理及其转基因耐盐棉花新种质的创造》2005-2007,主持
8.国家自然科学基金《棉花GhSAG基因的功能分析和分子机理研究》,2015-2018,第二位
9.973前期《玉米高温干旱抗性关键microRNA基因的分离及功能鉴定》,2014-2016,第二位
研究专利
1.棉花Na+/H+反向转运蛋白基因及其应用,专利号:ZL02151530.1
2.棉花金属硫蛋白基因GhMT1序列及其克隆与应用,专利号:ZL200510104301.X
3.一种棉花促分裂原蛋白激酶基因GhMAPK6及其应用,专利号:ZL201010590833.X
4.一种可提高转基因作物细菌抗性的基因GhMKK5及其应用,ZL201010590843.X
近期论文
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1.CailiWang,ShicaiZhang,ShengdongQi,ChengchaoZheng,ChangaiWu*(2015)DelayedgerminationofArabidopsisseedsunderchillingstressbyoverexpressinganabioticstressinducibleGhTPS11.GENE,doi:10.1016/j.gene.2015.08.056.
2.RuiXu,YuhanWang,HaoZheng,WeiLu,ChangaiWu,JinguangHuang,KangYan,GuodongYangandChengchaoZheng.(2015)Salt-inducedtranscriptionfactorMYB74isregulatedbytheRNA-directedDNAmethylationpathwayinArabidopsis.JournalofExperimentalBotany,66(19):5997-6008.
3.FengjuanJia,ChunyanWang,JinguangHuang,GuodongYang,ChangaiWuandChengchaoZheng.(2015)SCFE3ligasePP2-B11playsapositiveroleinresponsetosaltstressinArabidopsis.JournalofExperimentalBotany,66(15):4683–4697.
4.ChangtianChen,ChangaiWu1,JiamingMiao,YunxueLei,DongxiaoZhao,DanSun,GuodongYang,JinguangHuangandChengchaoZheng.(2014)ArabidopsisSAGproteincontainingtheMDN1domainparticipatesinseedgerminationandseedlingdevelopmentbynegativelyregulatingABI3andABI5.JournalofExperimentalBotany,65(1):35-45.
5.NanaMa,HailongFeng,XiaMeng,DongLi,DongyueYang,ChangaiWu*,QingweiMeng*.(2014)OverexpressionoftomatoSlNAC1transcriptionfactoraltersfruitpigmentationandsoftening.BMCPlantBiology,14:351.
6.YunxueLei,YaruFu,ChangtianChen,JinguangHuang,ChangaiWu*.(2014)OverexpressionofArabidopsiswali7domain-containingproteinASRproducesauxin-mediatedshort-rootphenotype.JournalofPlantGrowthRegulation,33:355-363.
7.YanjieLi,YiFang,YaruFu,JinguangHuang,ChangaiWu*,ChengchaoZheng*.(2013)NFYA1isinvolvedinregulationofpostgerminationgrowtharrestundersaltstressinArabidopsis.PLoSOne,8(4):e61289.
8.PengLiu,KangYan,YunXueLei,RuiXu,YueMinZhang,GuodongYang,JinguangHuang,ChangaiWu*,ChengchaoZheng*.(2013)TranscriptprofilingofmicroRNAsduringtheearlydevelopmentofthemaizebracerootviaSolexasequencing.Genomics,101:149-156.
9.KangYan,PengLiu,Chang-AiWu,Guo-DongYang,RuiXu,Qian-HuanGuo,Jin-GuangHuang,andCheng-ChaoZheng.(2012)Stress-InducedAlternativeSplicingProvidesaMechanismfortheRegulationofMicroRNAProcessinginArabidopsisthaliana.MolecularCell,48:521-531.
10.WeiLu,XiaoliTang,YanqingHuo,RuiXu,ShengdongQi,JingguangHuang,ChengchaoZheng,ChangaiWu*.(2012)Identificationandcharacterizationoffructose1,6-bisphosphatealdolasegenesinArabidopsisrevealsagenefamilywithdiverseresponsestoabioticstresses.Gene,503:65-74.
11.GangWang,QingguoZhu,QingweiMeng,ChangaiWu*.(2012)Transcriptprofilingduringsaltstressofyoungcotton(Gossypiumhirsutum)seedlingsviaSolexasequencing.ActaPhysiologiaePlantarum,34:107-115.
12.YanjieLi,YaruFu,JingguangHuang,ChangaiWu*,ChengchaoZheng*.(2011)TranscriptprofilingduringtheearlydevelopmentofthemaizebracerootviaSolexasequencing.TheFEBSJournal,278:156-166.
13.RuiruiXu,ShengdongQi,LongtaoLu,ChangtianChen,ChangaiWu*,ChengchaoZheng*.(2011)ADExD/HboxRNAhelicaseisimportantforpotassiumdeprivationresponsesandtoleranceinArabidopsisthaliana.TheFEBSJournal,278(13):2293-2306.
14.JingShi,LiangZhang,HailongAn,ChangaiWu*,XingqingGuo*.(2011)GhMPK16,anovelstress-responsivegroupDMAPKgenefromcotton,isinvolvedindiseaseresistanceanddroughtsensitivity.BMCMolecularBiology,12:22.
15.YanjieLi,YaruFu,LushaJi,ChangaiWu*,ChengchaoZheng*.(2010).CharacterizationandexpressionanalysisoftheArabidopsismir169family.PlantScience,178:271-280.
16.PeiLiu,GuodongYang,HongLi,ChangaiWu*.(2010)OverexpressionofNHX1sintransgenicArabidopsisenhancesphotoprotectioncapacityinhighsalinityanddroughtconditions.ActaPhysiologiaePlantarum,32:81-90.
17.HanhuaLiu,XinTian,YanjieLi,ChangaiWu*,ChengchaoZheng*.(2008)Microarray-basedAnalysisofStress-regulatedmicroRNAsinArabidopsisthaliana.RNA,14:836-843.
18.ChangaiWu,GuodongYang,QingweiMeng,ChengchaoZheng.(2004)ThecottonGhNHX1geneencodinganovelputativetonoplastNa+/H+antiporterplaysanimportantroleinsaltstress.Plant&CellPhysiology,45:600-607.