2025
6. Honghao Li#, Jiaming Zhang#, Jiaxin Gao, Yunzi Luo*. Advances in the microbial biosynthesis of L-tryptophan and its derivatives. Biodesign Research, 2025, 7(4): 100046. https://www.sciencedirect.com/science/article/pii/S2693125725000470
5. Jian Li#, Shiqing Zhang#, Honghao Li, Xiaoran Dai, Chaoqun Huang, He Ma, Huayi Liu, Qi Qi, Xiang Sheng*, Yunzi Luo*. Engineering phenylpyruvate decarboxylase for controlled biosynthesis of aromatic amino acid derivatives. Metabolic Engineering, 2025, 91: 466-479. https://www.sciencedirect.com/science/article/pii/S1096717625000977
4. Chaoqun Huang, Xuerui Jin, Zhenyu Liu, Can Cui, Yang Zhang, Binju Wang, Chun Zhang, Jianqiang Feng*, Dawen Niu* & Yunzi Luo*. Photoenzymatic enantioselective synthesis of fluorinated amides with remote stereocenter. Nature Communications, 2025, 16(1): 5753. https://www.nature.com/articles/s41467-025-60807-0
3. Jialu Li#, Huayi Liu#, Jian Li#, Juxiu Liu, Xinli Dai, Angqi Zhu, Qingjie Xiao, Wenyu Qian, Honghao Li, Li Guo, Chuangye Yan, Dong Deng*, Yunzi Luo* & Xiang Wang*. Cryo-EM structure of the β-1,3-glucan synthase FKS1-Rho1 complex. Nature Communications, 2025, 16(1): 2054. https://www.nature.com/articles/s41467-025-57152-7
2. Chaoqun Huang#, Xuerui Jin#, Yeyi Kan, Can Cui, Longchen Wei, Huayi Liu, Dawen Niu, Yunzi Luo*. Biocatalytic Synthesis of Aryl and Heteroaryl γ-Hydroxy-α-amino Acids via an Aldolase–Transaminase One-Pot Reaction. ACS Sustainable Chemistry & Engineering, 2025, 13(4): 1637-1647. https://doi.org/10.1021/acssuschemeng.4c08403
1. Yuhui Xie#, Xiaoyan Liu#, Tingting Wu#, Yunzi Luo*. Harnessing the streptomyces-originating type I-E CRISPR/Cas system for efficient genome editing in Streptomyces. Science China Life Sciences, 2025, 68(4): 1174-1182. https://engine.scichina.com/doi/10.1007/s11427-024-2677-4
2024
5. Qun Zhou#, Yatong Zhao#, Changqiang Ke#, Haojun Wang#, Sheng Gao, Hui Li, Yan Zhang,Yang Ye* & Yunzi Luo*. Repurposing endogenous type I-E CRISPRCas systems for natural product discovery in Streptomyces. Nature Communications.2024. 15: 9833. https://doi.org/10.1038/s41467-024-54196-z
4. Xuerui Jin#,Chaoqun Huang#, Can Cui, Huayi Liu, Zhenyu Liu, Dawen Niu, and Yunzi Luo*. Chemoenzymatic Synthesis of Fluorinated L‑α-Amino Acids. ACS Sustainable Chem. Eng. 2024. https://doi.org/10.1021/acssuschemeng.4c05318
3. Tianyu Zeng#, Chaoqun Huang#, Yang Zhang*, Yunzi Luo* and Dawen Niu*. C–H fluorination promoted by pyridine N-oxyl radicals. Green Chemistry, 2024, 26(15): 8701-8705.https://doi.org/10.1039/D4GC02247E
2. Zhenyu Liu, Huayi Liu, Chaoqun Huang, Qun Zhou, and Yunzi Luo*. Hybrid Cas12a variants with relaxed pam requirements expand genome editing compatibility. ACS Synth. Biol, 2024, https://doi.org/10.1021/acssynbio.4c00103
1. Hui Li#, Sheng Gao#, Sanyuan Shi#, Xiaomin Zhao, Haoyu Ye & Yunzi Luo*. Rational construction of genome-minimized Streptomyces host for the expression of secondary metabolite gene clusters. Synthetic and Systems Biotechnology, 2024, 9(3): 600-608 https://doi.org/10.1016/j.synbio.2024.04.017
2023
3. Jian Li#, Honghao Li# , Huayi Liu & Yunzi Luo*. Recent advances in the biosynthesis of natural sugar substitutes in yeast. J. Fungi 2023, 9, 907. https://doi.org/10.3390/jof9090907
2. Huayi Liu#, Qingjie Xiao, Xinxin Wu, He Ma, Jian Li, Xufan Guo, Zhenyu Liu, Yan Zhang & Yunzi Luo*. Mechanistic investigation of a D to N mutation in DAHP synthase that dictates carbon flux into the shikimate pathway in yeast. Communications chemistry, 2023, 6: 152. https://doi.org/10.1038/s42004-023-00946-x
1. Xufan Guo#, Xinxin Wu#, He Ma, Huayi Liu, Yunzi Luo*. Yeast: A platform for the production of L-tyrosine derivatives. Yeast, 2023. https://doi.org/10.1002/yea.3850
2022
6. Sanyuan Shi#, Jingrui Tian#, Yunzi Luo*. Recent advances in fluorinated products biosynthesis. Bioresource Technology Reports, 2022, 20: 101288. https://doi.org/10.1016/j.biteb.2022.101288
5. Huayi Liu, Xinxin Wu, He Ma, Jian Li, Zhenyu Liu, Xufan Guo, Jia Dong, Shaolan Zou, Yunzi Luo*. High-Level Production of Hydroxytyrosol in Engineered Saccharomyces cerevisiae. ACS Synthetic Biology, 2022, 11(11): 3706-3713. https://doi.org/10.1021/acssynbio.2c00316
4. Huayi Liu, Yi Zhou#, Yingjie Song#, Qianqian Zhang#, Yeyi Kan, Xinyue Tang, Qingjie Xiao, Qianyin Xiang, Huanxiang Liu*, Yunzi Luo*, and Rui Bao*. Structural and Dynamics Studies of the Spcas9 Variant Provide Insights into the Regulatory Role of the REC1 Domain. ACS Catalysis, 2022, 12: 8687-8697. https://doi.org/10.1021/acscatal.2c01804
3. Sanyuan Shi#, Yuhui Xie#, Gaoli Wang, Yunzi Luo*. Metabolite-based biosensors for natural product discovery and overproduction. Current Opinion in Biotechnology, 2022, 75. https://doi.org/10.1016/j.copbio.2022.102699
2. Chaoqun Huang#, Chang Wang#, Yunzi Luo*. Research progress of pathway and genome evolution in microbes. Synthetic and Systems Biotechnology, 2022, 7(1): 648-656. https://doi.org/10.1016/j.synbio.2022.01.004.
1. Xuerui Jin, Jixin Dong, Xufan Guo, Mingzhu Ding, Rui Bao*, Yunzi Luo*. Current advances in polyurethane biodegradation. Polym Int. 2022. https://doi.org/10.1002/pi.6360
2021
3. Jing Yang#, Yujuan Tian#, Huayi Liu, Yeyi Kan, Yi Zhou, Ying Wang, Yunzi Luo*. Harnessing the Endogenous 2μ Plasmid of Saccharomyces cerevisiae for Pathway Construction. Frontiers in Microbiology, 2021, 12: 679665. https://doi.org/10.3389/fmicb.2021.679665
2. Zhenyu Liu#, Yatong Zhao#, Chaoqun Huang, Yunzi Luo*. Recent Advances in Silent Gene Cluster Activation in Streptomyces. Frontiers in Bioengineering and Biotechnology, 2021, 9:632230. https://doi.org/10.3389/fbioe.2021.632230
1. Yeyi Kan, Lihui He, Yunzi Luo*, Rui Bao*. IsPETase Is a Novel Biocatalyst for Poly(ethylene terephthalate) (PET) Hydrolysis. ChemBioChem. 2021, 22 (10), 1706-1716. https://doi.org/10.1002/cbic.202000767
2020
6. D. Zhang, X. Wang, Y. Ye, Y. He, F. He, Y. Tian, Y. Luo*, S. Liang*. Label-Free Proteomic Dissection on Dptp-Deletion Mutant Uncovers Dptp Involvement in Strain Growth and Daptomycin Tolerance of Streptomyces Roseosporus. Microbial Biotechnology, 2020, 14(2), 708-725. https://doi.org/10.1111/1751-7915.13736
5. J. Lian*, Y. Wang*, Y. Luo*, C. Li*. Editorial: Development and Application of Novel Genome Engineering Tools in Microbial Biotechnology. Frontiers in Bioengineering and Biotechnology, 2020, 8:621851. https://doi.org/10.3389/fbioe.2020.621851
4. B. Liu#, C. Zhao#, Q. Xiang, N. Zhao, Y. Luo*, R. Bao*. Structural and Biochemical Studies of the Glycosyltransferase Bs-Yjic from Bacillus Subtilis. International Journal of Biological Macromolecules, 2020, 166, 806-817. https://doi.org/10.1016/j.ijbiomac.2020.10.238
3. H. Liu, Y. Tian, Y. Zhou, Y. Kan, T. Wu, W. Xiao, Y. Luo*. Multi-modular Engineering of Saccharomyces Cerevisiae for High-titre Production of Tyrosol and Salidroside. Microbial Biotechnology, 2020, 14 (6), 2605-2616. https://doi.org/10.1111/1751-7915.13667
2. J. Zhang, D. Zhang, J. Zhu, H. Liu, S. Liang*, Y. Luo*. Efficient Multiplex Genome Editing in Streptomyces via Engineered CRISPR-Cas12a Systems. Frontiers in Bioengineering and Biotechnology, 2020, 8:726. https://doi.org/10.3389/fbioe.2020.00726
1. Q. Zhou#, S. Ning#, Y. Luo*. Coordinated Regulation for Nature Products Discovery and Overproduction in Streptomyces. Synthetic and Systems Biotechnology, 2020, 5(2): 49-58. https://doi.org/10.1016/j.synbio.2020.04.002
2019
2. L. Wang, H. Wang, H. Liu, Q. Zhao, B. Liu, L. Wang, J. Zhang, J. Zhu, R. Bao, Y. Luo*. Improved CRISPR-Cas12a-assisted One-pot DNA Editing Method Enables Seamless DNA Editing. Biotechnology and Bioengineering, 2019, 116(6), 1463-1474. https://doi.org/10.1002/bit.26938
1. Q. Zhao, L. Wang, Y. Luo*. Recent Advances in Natural Products Exploitation in Streptomyces via Synthetic Biology. Engineering in Life Sciences, 2019, 19(6): 452-462. https://doi.org/10.1002/elsc.201800137
2018
3. H. Liu, L. Wang, Y. Luo*. Blossom of CRISPR Technologies and Applications in Disease Treatment. Synthetic and Systems Biotechnology, 2018, 3(4), 217-228. https://doi.org/ 10.1016/j.synbio.2018.10.003
2. H. Yu, Z. Wang, H. Xu, J. Guo, Q. Ma, X. Mu, Y. Luo*. A Method for Absolute Protein Expression Quantity Measurement Employing Insulator RiboJ. Engineering. 2018, 4(6), 881-887. https://doi.org/10.1016/j.eng.2018.09.012
1. B. Liu, L. He, L. Wang, T. Li, C. Li, H. Liu, Y. Luo*, R. Bao*. Protein Crystallography and Site-Direct Mutagenesis Analysis of the Poly (ethylene terephthalate) Hydrolase PETase from Ideonella sakaiensis. ChemBioChem, 2018, 19(14), 1471-1475. https://doi.org/10.1002/cbic.201800097
Before 2018
15. Y. Luo, B. Enghiad and H. Zhao*. New Tools for Reconstruction and Heterologous Expression of Natural Product Biosynthetic Gene Clusters. Natural Product Reports, 2016, 33(2): 174-182. https://doi.org/10.1039/c5np00085h
14. Y. Luo#, B. Li#, D. Liu#, L. Zhang, Y. Chen, B. Jia, B. Zeng, H. Zhao* and Y. Yuan*. Engineered Biosynthesis of Natural Products in Heterologous Hosts. Chemical Society Reviews, 2015, 44(15): 5265-90. https://doi.org/10.1039/c5cs00025d
13. Y. Luo, L. Zhang, K. Barton and H. Zhao*. Systematic Identification of a Panel of Strong Constitutive Promoters from Streptomycesalbus. ACS Synthetic Biology, 2015, 4(9):1001-10. https://doi.org/10.1021/acssynbio.5b00016
12. Y. Luo#, R. Cobb# and H. Zhao*. Recent Advances in Natural Product Discovery. Current Opinion in Biotechnology, 2014, 30, 230-237. https://doi.org/10.1016/j.copbio.2014.09.002
11. Y. Luo, H. Huang, J. Liang, M. Wang, L. Lu, Z. Shao, R. Cobb and H. Zhao*. Activation and Characterization of a Cryptic Polycyclic Tetramate Macrolactam Biosynthetic Gene Cluster. Nature Communications, 2013, 4, 2894. https://doi.org/10.1038/ncomms3894
10. Y. Luo, J. Lee, and H. Zhao*. Challenges and Opportunities in Synthetic Biology for Chemical Engineers. Chemical Engineering Science, 2013, 103, 115-119. https://doi.org/10.1016/j.ces.2012.06.013
9. J. Liang#, Y. Luo#, and H. Zhao*. Synthetic Biology: Putting Synthesis into Biology. Wiley Interdisciplinary Reviews: Systems Biologyand Medicine, 2011, 3(1), 7-20. https://doi.org/10.1002/wsbm.104
8. S. Rubin-Pitel#, Y. Luo#, J. Lee, and H. Zhao*. A Diverse Family of Type III Polyketide Synthases in Eucalyptus Species. Molecular Biosystems, 2010, 6, 1444-1446. https://doi.org/10.1039/c004992a
7. T. Si, Y. Luo, Z. Bao and H. Zhao*. RNAi-assisted Genome Evolution in Saccharomyces cerevisiae for Complex Phenotype Engineering. ACS Synthetic Biology, 2014, 4(3), 283-91. https://doi.org/10.1021/sb500074a
6. T. Si, Y. Luo, H. Xiao, and H. Zhao*. Utilizing an Endogenous Pathway for 1-Butanol Production in Saccharomyces cerevisiae. Metabolic Engineering, 2014, 22, 60-8. https://doi.org/10.1016/j.ymben.2014.01.002
5. Z. Shao, G. Rao, C. Li, Z. Abil, Y. Luo and H. Zhao*. Refactoring Silent Biosynthetic Gene Clusters Using a Plug-and-Play Scaffold. ACS Synthetic Biology, 2013, 2(11), 662-9. https://doi.org/10.1021/sb400058n
4. R. E. Cobb, Y. Luo, T. Freestone and H. Zhao*. Chapter 10: Drug Discovery and Development via Synthetic Biology. In Synthetic Biology: Tools and Applications, (H. Zhao, Ed.), pp. 183-206, Elsevier, San Diego, CA, 2013.
3. Z. Shao, Y. Luo, and H. Zhao*. DNA Assembler Method for Construction of Zeaxanthin-Producing Saccharomyces cerevisiae Strains. Methods in Molecular Biology, 2012, 898, 251-262. https://doi.org/10.1007/978-1-61779-918-1_17
2. Z. Shao, Y. Luo, and H. Zhao*. Rapid Characterization and Engineering of Natural Product Biosynthetic Pathways via DNA Assembler. Molecular Biosystems, 2011, 7, 1056-1059. https://doi.org/10.1039/c0mb00338g
1. J. Li, Y. Luo, J. Lee, and H. Zhao*. Cloning and Characterization of a Type III Polyketide Synthase from Aspergillus niger. Bioorganic & Medicinal Chemistry Letters, 2011, 21(20),6085-6089. https://doi.org/10.1016/j.bmcl.2011.08.058
