个人简介

Dr. Gober received his B.S. from Northeastern University and his Ph.D. from Boston University. He was a postdoctoral fellow at Columbia University and Stanford University.

研究领域

Biochemistry/Systems Biology and Biological Regulation/Development and Gene Regulation

Fundamental problems in developmental biology, such as the generation of asymmetry, differential transcription, and the execution of positional information, are all exhibited during the cell cycle of the bacterium, Caulobacter crescentus. Caulobacter undergoes a fixed sequence of differentiation events within each cell cycle. Cell division yields morphologically and biochemically dissimilar daughter cells; a swarmer cell which posesses a single polar flagellum and a non-motile stalked cell. During the latter portion of the DNA replication phase, the components of the flagellum are synthesized and assembled at the pole of the predivisional cell that is opposite the stalk. For a subset of flagellar genes, positional information is generated through localized transcription in one pole of the cell. The newly replicated chromosomes in the predivisional cell, therefore differ in their program of transcription as well as the ability to initiate DNA replication. The overall goal of our research program is to define the mechanisms by which this differential, global programming of the Caulobacter chromosome is accomplished. Using both genetic and biochemical approaches we are determining the mechanisms of both temporal and spatial transcriptional activation of these locally expressed promoters. We are also interested in the mechanisms that couple cellular morphogenesis to gene expression. Early flagellar assembly events are required for the transcription of genes encoding structures that are assembled later in the flagellum. These same events are also required for normal cell division. We are determining how factors that regulate gene expression and cell division monitor the assembly of the cellular structures.

近期论文

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Clarke, C.F., Rowat,A.C., J.W. Gober Osmotic stress: Is CoQ a membrane stabilizer? Nat Chem Biol. (2014) Mar 18;10(4):242-3. White, C.L., J.W. Gober MreB: pilot or passenger of cell wall synthesis? Trends Microbiol. (2012) Feb;20(2):74-9. White Courtney L, Kitich Aleksandar, Gober James W Positioning cell wall synthetic complexes by the bacterial morphogenetic proteins MreB and MreD. Molecular microbiology, 2010; 76(3): 616-33. England Jennifer C, Perchuk Barrett S, Laub Michael T, Gober James W Global regulation of gene expression and cell differentiation in Caulobacter crescentus in response to nutrient availability. Journal of bacteriology, 2010; 192(3): 819-33. Divakaruni Arun V, Baida Cyril, White Courtney L, Gober James W The cell shape proteins MreB and MreC control cell morphogenesis by positioning cell wall synthetic complexes. Mol. Microbiol., 2007; 66(1): 174-88. Divakaruni Arun V, Loo Rachel R Ogorzalek, Xie Yongming, Loo Joseph A, Gober James W The cell-shape protein MreC interacts with extracytoplasmic proteins including cell wall assembly complexes in Caulobacter crescentus. Proc. Natl. Acad. Sci. U.S.A., 2005; 102(51): 18602-7. Dutton Rachel J, Xu Zhaohui, Gober James W Linking structural assembly to gene expression: a novel mechanism for regulating the activity of a sigma54 transcription factor. Mol. Microbiol., 2005; 58(3): 743-57. Muir Rachel E, Easter Jesse, Gober James W The trans-acting flagellar regulatory proteins, FliX and FlbD, play a central role in linking flagellar biogenesis and cytokinesis in Caulobacter crescentus. Microbiology (Reading, Engl.), 2005; 151(Pt 11): 3699-711. England Jennifer C, Gober James W Role of core promoter sequences in the mechanism of swarmer cell-specific silencing of gyrB transcription in Caulobacter crescentus. BMC Microbiol., 2005; 5(1): 25. Llewellyn Midge, Dutton Rachel J, Easter Jesse, O'donnol Danielle, Gober James W The conserved flaF gene has a critical role in coupling flagellin translation and assembly in Caulobacter crescentus. Mol. Microbiol., 2005; 57(4): 1127-42.