Kolpashchikov, Dmitry - University of Central Florida

个人简介

1.1. EDUCATION 1999 – PhD, Bioorganic Chemistry, Novosibirsk Institute of Bioorganic Chemistry, Russia Dissertation: ‘Synthesis of photoreactive dNTP analogs and their application for the study of protein-nucleic acid interactions’. Advisor: Olga I. Lavrik 1994 – BS, Chemistry, Novosibirsk State University, Russia Thesis: ‘Synthesis of dTTP analogs conjugated with photoreactive arylazido groups’ Advisor: Tatyana S. Godovikova 1.2. POSTDOCTORAL TRAINING 2002-2003 – Postdoctoral Research Scientist, Columbia University, New York, USA Aptameric sensors and molecular computation Research advisors: Milan N. Stojanovic and Donald W. Landry 2000-2002 – Postdoctoral Research Fellow, National Institute of Genetics, Japan, Study of molecular architecture of influenza virus RNA polymerase Research advisor: Akira Ishihama 1.3. PROFESSIONAL EMPLOYMENT 2014-present – Associate Professor, University of Central Florida, Orlando, FL, USA 2008-2014 – Assistant Professor, University of Central Florida, Orlando, FL, USA 2003-2008 – Associate Research Scientist, Columbia University, New York, NY, USA 1.4. HONORS AND AWARDS 2013-2014 – Research Incentive Award (RIA) University of Central Florida. RIA rewards outstanding achievements in research, scholarly and creative activities; adds $5,000.00 to base salary 2000-2003 – Young Talented Scientist Award of Siberian Branch of Russian Academy of Science 2001-2002 – Postdoctoral Fellowship from Centre of Excellence (COE) program of the Ministry of Education, Culture, Sport, Science, and Technology of Japan 2000-2001 – Postdoctoral Fellowship from Centre of Excellence (COE) program of the Ministry of Education, Culture, Sport, Science, and Technology of Japan 2 1.5. INTERNATIONAL RESEARCH PROGRAMS AND COLLABORATIONS 2013-present – Maxim V. Berezovski, University of Ottawa, Ottawa, Canada 2010-Present – John Katz, Scheme lab Inc., Sao Paulo, Brazil on the development of point of care diagnostics 2000-2002 – International Academic Exchange Program (Centre of Excellence (COE) program of the Ministry of Education, Culture, Sport, Science, and Technology of Japan) in Akira Ishihama’s lab 1999 – Visiting Scientist, NIEHS, NC, USA in Samuel H Wilson’s lab 1999 – International Exchange Program between Russian Academy of Science and French Academy of Science (CNRS) Convention de’Exchange in Alan Favre’s lab 1998 – International Exchange Program between Russian Academy of Science and French Academy of Science (CNRS) Convention de’Exchange in Alan Favre’s lab

研究领域

Our research interests cover all aspects of chemistry and biochemistry of nucleic acids and nucleic acids binding proteins. The following projects are in progress. Multicomponent sensors for nucleic acid analysis. This project aims at developing new hybridization probes for real-time analysis of specific DNA and RNA sequences. A multicomponent sensor produces detectable signal only when all sensor components form specific complexes with the analyzed DNA or RNA. In multicomponent sensors each constituent part can be designed to address a particular issue of target recognition. The high specialization of each component leads to the improved accuracy of genotyping for polymorphic sites, detection of folded nucleic acids and reduces the cost of multiplex assays. The sensors promise to advance molecular diagnostics of human genetic and infectious diseases. Binary reagents for DNA polymerase inactivation. . We develop two-component nucleotide reagents for highly selective irreversible inhibition of human immunodeficiency virus (HIV) reverse transcriptase (RT). The approach promises to deliver new type of efficient and side effect-free anti-AIDS drugs. DNA logic circuits. DNA-based molecular logic gates that can be used in the first DNA-based computer processor are under investigation. The main advantage of the gate design is the possibility to connect logic units in complex circuits at the molecular level.

近期论文

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Gerasimova Y.V., Kolpashchikov D.M. (2014) Enzyme-assisted target recycling (EATR) for nucleic acid detection. Chemical Society Reviews. Bengtson H.B., Kolpashchikov D.M. (2014) A Differential Fluorescent Receptor for Nucleic Acid Analysis ChemBioChem, 15, 228-231. Labib M., Ghobadloo S.M., Khan N., Kolpashchikov D.M., Berezovski M.V. (2013) Four-Way Junction Formation Promoting Ultrasensitive Electrochemical Detection of MicroRNA. Anal. Chem. 85, 9422-9427. Gerasimova Y.V., Kolpashchikov D.M. (2013) Folding 16S RNA in a signal-producing structure for detection of bacteria, Angewandte Chemie International Edition Engl. 52, 10586-10588. This paper was featured on the cover of a September 2013 issue of the journal. Gerasimova Y.V., Cornett E.M., Edwards E., Su X., Rohde K.H., Kolpashchikov D.M. (2013) Deoxyribozyme Cascade for Visual Detection of Bacterial RNA. ChemBioChem. 14, in press, DOI: 10.1002/cbic.201300471. Cornett E.M., O’steen M.R, Kolpashchikov D.M. (2013) Operating cooperatively (OC) sensor for highly specific recognition of nucleic acids. PLOS one 8, e55919. Cornett E.M., Gerasimova Y.V., Kolpashchikov D.M. (2013) Two-component covalent inhibitor: Bioorganic & Medicinal Chemistry, 21, 1988–1991. Gerasimova Y.V., Kolpashchikov D.M. (2013) Detection of bacterial 16S rRNA using a molecular beacon-based X sensor. Biosensors & Bioelectronics, 41, 386–390. Cornett E.M., Kolpashchikov D.M.* SNP analysis using a molecular beacon-based operating cooperatively (OC) sensor. Methods in Molecular Biology, Nucleic acid detection, Humana press, 2013. 1039, 81-86. Gerasimova Y.V.,* Ballantyne J., Kolpashchikov D.M. Detection of SNP-Containing human DNA sequences using a split sensor with universal molecular beacon reporter. Methods in Molecular Biology, Nucleic acid detection, Humana press, 2013. 1039, 69-80. Gerasimova Y.V., Kolpashchikov D.M. (2012) Connectable DNA Logic Gates: OR and XOR logics. Chemistry-An Asian Journal, 7, 534-540. Cornett E.M., Campbell E.A., Gulenay G., Peterson E, Bhaskar N, Kolpashchikov D.M. (2012) Molecular logic gates for DNA analysis: detection of rifampin resistance in M.tuberculosis DNA. Angew Chem Int Ed Engl. 51, 9075-9077 Kolpashchikov D.M. (2012) An Elegant Biosensor Molecular Beacon Probe: Challenges and Recent Solutions, Scientifica, 2012, ID 928783. Kolpashchikov D.M., Gerasimova Y.V., Khan M.S. (2011) DNA nanotechnology for nucleic acid analysis: DX motif-based sensor ChemBioChem, 12, 2564-2567. Nguyen C., Grimes J., Gerasimova Y.V., Kolpashchikov D.M. (2011) Molecular beacon-based tricomponent probe for SNP analysis in folded nucleic acids, Chemistry: A European J. 17, 13052-13058. Gerasimova Y.V., Peck S. Kolpashchikov D.M. (2010) Enzyme-Assisted Binary Probe for Sensitive Detection of RNA and DNA. Chem Commun. 46: 8761-8763. Grimes J., Gerasimova Y.V. and Kolpashchikov D.M. (2010) Real-Time SNP Analysis in Secondary Structure-Folded Nucleic Acids, Angew Chem Int Ed Engl. 49: 8950-8953. This article was rated by Faculty of 1000 (Uttamchandani M, Yao S: 2010. F1000.com/6959956 http://f1000.com/6959956#eval7154054) Kolpashchikov D.M. (2010) Binary Probes for Nucleic Acid Analysis, Chem. Rev. 110, 4709-4723. Gerasimova Y.V., Hayson A., Ballantyne, J., Kolpashchikov D.M. (2010) A Single Molecular Beacon Probe is Sufficient for the Analysis of Multiple Nucleic Acid Sequences, ChemBioChem, 11, 1762-1768. Lake A., Shang S., Kolpashchikov D.M. (2010) Molecular Logic Gates Connected via DNA Four Way Junctions, Angew. Chemie Int. Ed. Engl., 49, 4459-4462.