研究领域

Bioinorganic/Biophysical/Chemical Biology

The Crane group studies structure, function and mechanism of protein systems that underlie signal transduction. Of particular emphasis are processes mediated by redox and photochemistry and those dependent on highly cooperative macromolecular assemblies. Projects include understanding circadian clock light sensors, bacterial transmembrane signaling, nitric oxide enzymology and general aspects of protein electron transfer.

近期论文

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Davydov R, Labby KJ, Chobot SE, Lukoyanov DA, Crane BR, Silverman RB, Hoffman BM. Enzymatic and Cryoreduction EPR Studies of the Hydroxylation of Methylated Nω-Hydroxy-l-arginine Analogues by Nitric Oxide Synthase from Geobacillus stearothermophilus. Biochemistry. 2014 Oct 8. [Epub ahead of print] PubMed PMID: 25251261. Merz GE, Borbat PP, Pratt AJ, Getzoff ED, Freed JH, Crane BR. Copper-Based Pulsed Dipolar ESR Spectroscopy as a Probe of Protein Conformation Linked to Disease States. Biophys J. 2014 Oct 7;107(7):1669-74. doi: 10.1016/j.bpj.2014.07.068. PubMed PMID: 25296320. Conrad KS, Manahan CC, Crane BR. Photochemistry of flavoprotein light sensors. Nat Chem Biol. 2014 Sep 17;10(10):801-809. doi: 10.1038/nchembio.1633. PubMed PMID: 25229449. Crane BR, Young MW.Interactive features of proteins composing eukaryotic circadian clocks. Annu Rev Biochem. 2014;83:191-219. doi: 10.1146/annurev-biochem-060713-035644. PubMed PMID: 24905781. Vaidya AT, Top D, Manahan CC, Tokuda JM, Zhang S, Pollack L, Young MW, Crane BR. Flavin reduction activates Drosophila cryptochrome. Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):20455-60. doi: 10.1073/pnas.1313336110. Epub 2013 Dec 2. PubMed PMID: 24297896; PubMed Central PMCID: PMC3870761. Jiang N, Kuznetsov A, Nocek JM, Hoffman BM, Crane BR, Hu X, Beratan DN. Distance-independent charge recombination kinetics in cytochrome c-cytochrome c peroxidase complexes: compensating changes in the electronic coupling and reorganization energies. J Phys Chem B. 2013 Aug 8;117(31):9129-41. doi: 10.1021/jp401551t. Epub 2013 Jul 29. PubMed PMID: 23895339; PubMed Central PMCID: PMC3809023. Piasta KN, Ulliman CJ, Slivka PF, Crane BR, Falke JJ. Defining a key receptor-CheA kinase contact and elucidating its function in the membrane-bound bacterial chemosensory array: a disulfide mapping and TAM-IDS Study. Biochemistry. 2013 Jun 4;52(22):3866-80. doi: 10.1021/bi400385c. Epub 2013 May 23. PubMed PMID: 23668882; PubMed Central PMCID: PMC3705729. Li X, Fleetwood AD, Bayas C, Bilwes AM, Ortega DR, Falke JJ, Zhulin IB, Crane BR. The 3.2 Å resolution structure of a receptor: CheA:CheW signaling complex defines overlapping binding sites and key residue interactions within bacterial chemosensory arrays. Biochemistry. 2013 Jun 4;52(22):3852-65. doi: 10.1021/bi400383e. Epub 2013 May 23. PubMed PMID: 23668907; PubMed Central PMCID: PMC3694592. Sircar R, Greenswag AR, Bilwes AM, Gonzalez-Bonet G, Crane BR. Structure and activity of the flagellar rotor protein FliY: a member of the CheC phosphatase family. J Biol Chem. 2013 May 10;288(19):13493-502. doi: 10.1074/jbc.M112.445171. Epub 2013 Mar 26. PubMed PMID: 23532838; PubMed Central PMCID: PMC3650386. Levy C, Zoltowski BD, Jones AR, Vaidya AT, Top D, Widom J, Young MW, Scrutton NS, Crane BR, Leys D. Updated structure of Drosophila cryptochrome. Nature. 2013 Mar 21;495(7441):E3-4. doi: 10.1038/nature11995. PubMed PMID: 23518567; PubMed Central PMCID: PMC3694752. Airola MV, Huh D, Sukomon N, Widom J, Sircar R, Borbat PP, Freed JH, Watts KJ, Crane BR. Architecture of the soluble receptor Aer2 indicates an in-line mechanism for PAS and HAMP domain signaling. J Mol Biol. 2013 Mar 11;425(5):886-901. doi: 10.1016/j.jmb.2012.12.011. Epub 2012 Dec 26. PubMed PMID: 23274111; PubMed Central PMCID: PMC3577987. Airola MV, Sukomon N, Samanta D, Borbat PP, Freed JH, Watts KJ, Crane BR. HAMP domain conformers that propagate opposite signals in bacterial chemoreceptors. PLoS Biol. 2013;11(2):e1001479. doi: 10.1371/journal.pbio.1001479. Epub 2013 Feb 12. PubMed PMID: 23424282; PubMed Central PMCID: PMC3570549. Conrad KS, Bilwes AM, Crane BR. Light-induced subunit dissociation by a light-oxygen-voltage domain photoreceptor from Rhodobacter sphaeroides. Biochemistry. 2013 Jan 15;52(2):378-91. doi: 10.1021/bi3015373. Epub 2013 Jan 3. PubMed PMID: 23252338; PubMed Central PMCID: PMC3582384. McLaughlin MP, Retegan M, Bill E, Payne TM, Shafaat HS, Peña S, Sudhamsu J, Ensign AA, Crane BR, Neese F, Holland PL. Azurin as a protein scaffold for a low-coordinate nonheme iron site with a small-molecule binding pocket. J Am Chem Soc. 2012 Dec 5;134(48):19746-57. doi: 10.1021/ja308346b. Epub 2012 Nov 20. PubMed PMID: 23167247; PubMed Central PMCID: PMC3515693. Barry SM, Kers JA, Johnson EG, Song L, Aston PR, Patel B, Krasnoff SB, Crane BR, Gibson DM, Loria R, Challis GL. Cytochrome P450–catalyzed L-tryptophan nitration in thaxtomin phytotoxin biosynthesis. Nat Chem Biol. 2012 Oct;8(10):814-6. PubMed PMID: 22941045; PubMed Central PMCID: PMC3522571. Crane BR. Biochemistry. Nature’s intricate clockwork. Science. 2012 Jul 13;337(6091):165-6. doi: 10.1126/science.1224611. PubMed PMID: 22798591. Park S, Kim KY, Kim S, Crane BR. Crystallization and preliminary X-ray crystallographic analysis of Thermotoga maritima CheA P3-P4-P5 domains in complex with CheW. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jun 1;68(Pt 6):713-5. doi: 10.1107/S174430911201826X. Epub 2012 May 24. PubMed PMID: 22684078; PubMed Central PMCID: PMC3370918. Bhatnagar J, Sircar R, Borbat PP, Freed JH, Crane BR. Self-association of the histidine kinase CheA as studied by pulsed dipolar ESR spectroscopy. Biophys J. 2012 May 2;102(9):2192-201. doi: 10.1016/j.bpj.2012.03.038. PubMed PMID: 22824284; PubMed Central PMCID: PMC3341552. Briegel A, Li X, Bilwes AM, Hughes KT, Jensen GJ, Crane BR. Bacterial chemoreceptor arrays are hexagonally packed trimers of receptor dimers networked by rings of kinase and coupling proteins. Proc Natl Acad Sci U S A. 2012 Mar 6;109(10):3766-71. doi: 10.1073/pnas.1115719109. Epub 2012 Feb 21. PubMed PMID: 22355139; PubMed Central PMCID: PMC3309718. Zoltowski BD, Vaidya AT, Top D, Widom J, Young MW, Crane BR. Structure of full-length Drosophila cryptochrome. Nature. 2011 Nov 13;480(7377):396-9. doi: 10.1038/nature10618. Erratum in: Nature. 2013 Apr 11;496(7444):252. PubMed PMID: 22080955; PubMed Central PMCID: PMC3240699.