Rainey, Jan K. - Dalhousie University - Department of Biochemistry & Molecular Biology

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Rainey, Jan K. Professor 收藏完善纠错
Dalhousie University Department of Biochemistry & Molecular Biology
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研究领域

Structural & biophysical studies of membrane proteins & fibrous proteins Proteins in the cell membrane and in fibres function in highly organized supramolecular assemblies, groupings of many molecules. Both classes of protein are critical for life, but are notoriously difficult to study. Our research goal is to determine not only how these classes of proteins function, but to carry out studies of these proteins in highly physiologically relevant environments. The primary aim is to understand the contribution of each constituent atom to the biological activity of a protein, providing fundamental understanding of biochemical processes, new routes for drug design, and alternative approaches for disease diagnosis and treatment. Current systems under study include a G-protein coupled receptor and its peptidic ligands; recombinant spider silk (one of the toughest known materials!); the extracellular matrix protein collagen (the most abundant protein in animals); soft polymeric nanoparticles; and, fusion-associate small transmembrane (FAST) proteins (a recently discovered class of viral membrane fusion proteins.) Protein preparation Two major approaches are used in our laboratory to produce proteins. In the organic chemistry based solid-phase peptide synthesis, a polypeptide is built by adding each amino acid in sequence. This provides us the ability to alter or tailor any individual amino acid residue we wish, either to determine effects of substitutions or to allow highly specific stable-isotope for nuclear magnetic resonance (NMR) spectroscopy or fluorescent labelling for biophysical study. Alternatively, molecular biology based cloning and expression is used to produce larger proteins. Beyond production of proteins, this readily allows both uniform and selective stable-isotope labelling . In each case, purification may be performed by high-performance liquid chromatography (HPLC) or other chromatographic methods, as appropriate. Mass spectrometry is typically used to characterize our synthetic or expression products. NMR spectroscopy & structure calculations NMR spectroscopy is our primary experimental technique, allowing us to determine protein structure and dynamics at the level of the individual nucleus. We use both solution-state and solid-state NMR to understand protein function in a variety of situations ranging from polypeptides in aqueous buffer to large protein assemblies and proteins embedded in phospholipid bilayers. In the case of solid-state NMR, we are currently working towards new methods that will allow characterization of not only the structure of proteins in membranes or fibres but the structure of these proteins relative to their supramolecular environment. Structure calculations are a critical component of biomolecular NMR, and we spend a lot of time ensuring that our calculations are truly providing a structural ensemble accurately representing the experimental data. Scanning probe microscopy & force spectroscopy Scanning probe microscopy (SPM), also known as atomic force microscopy (AFM), is the other major technique employed by our laboratory. This type of microscopy uses a very sharp tip to image a surface, making use of the force of interaction between the tip and the surface to produce a topological and/or chemical image of the surface. This technique is capable of probing individual atoms, but for biological samples a more realistic resolution limit is on the order of a nanometer. SPM can also be used for study of the interaction forces between pairs of molecules, providing picoNewton level accuracy. A major focus in our research is the development of methods to allow exactly the same sample to be studied by solid-state NMR and SPM. Circular dichroism & fluorescence spectroscopy Circular dichroism (CD) spectroscopy allows rapid determination of protein secondary structure under various conditions. Fluorescence spectroscopy allows us to quickly assess the environment surrounding individual amino acids (i.e. are they exposed to solvent vs. buried in a protein core?) and the free- or bound-state of a protein. These methods provide an important counterpoint to our NMR and SPM experiments, since characterization is often much more rapidly achieved and provide different classes of information.

近期论文

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Huang, S.K., Shin, K., Sarker, M. and Rainey, J.K., (2017) Apela exhibits isoform- and headgroup-dependent modulation of micelle binding, peptide conformation and dynamics. Biochim Biophys Acta - Biomembranes 1859:767-778 [PubMed] [Article] Zhong, X.Z., Zou, Y., Sun, X., Dong, G., Cao, Q., Pandey, A., Rainey, J.K., Zhu, X. and Dong, X-P., (2017) Inhibition of TRPML1 by lysosomal adenosine involved in severe combined immunodeficiency diseases. J. Biol. Chem. 292:3445-3455 [PubMed] [Article] Young, B.M., Nguyen, E., Chedrawe, M.A.J., Rainey, J.K. and Dupré, D.J., (2017) Differential contribution of transmembrane domains IV, V, VI, and VII to human angiotensin II type 1 receptor homomer formation J. Biol. Chem. 292:3341-3350 [PubMed] [Article] Shin, K., Chapman, N.A., Sarker, M., Kenward, K., Huang, S.K., Weatherbee-Martin, N., Pandey, A., Dupré, D.J. and Rainey, J.K., (2017) Bioactivity of the putative apelin proprotein expands the repertoire of apelin receptor ligands. Biochim Biophys Acta - General Subjects 1861:1901-1912 [PubMed] [Article] Patterson, R.E., Weatherbee-Martin, N. and Rainey, J.K., (2017) Pyrene-Apelin Conjugation Modulates Fluorophore- and Peptide-Micelle Interactions. J Phys Chem B 121:4768–4777 [PubMed] [Article] Langelaan, D.N., Pandey, A., Sarker, M. and Rainey, J.K., (2017) Preserved transmembrane segment topology, structure, and dynamics in disparate micellar environments J Phys Chem Lett 8:2381-2386 [PubMed] [Article] Shin, K., Sarker, M., Huang, S.K., and Rainey, J.K., (2017) Apelin conformational and binding equilibria upon micelle interaction primarily depend on membrane-mimetic headgroup Sci Rep Under review: Shin, K., Kenward, K. and Rainey, J.K., (2017) Apelinergic system structure and function Compr Physiol Under review: Tremblay, M-L., Xu, L., Sarker, M., Liu, X-Q. and Rainey, J.K., (2016) Characterizing aciniform silk repetitive domain backbone dynamics and hydrodynamic modularity. Int. J. Mol. Sci. 147:E1305 [PubMed] [Article] Pandey, A., Shin, K., Patterson, R.E., Liu, X-Q. and Rainey, J.K., (2016) Current strategies for protein production and purification enabling membrane protein structural biology. Biochem. Cell Biol. 94(6):507-527 [PubMed] [Article] Weatherbee-Martin, N., Xu, L., Hupe, A., Kreplak, L., Fudge, D.S., Liu, X-Q. and Rainey, J.K., (2016) Identification of wet-spinning and post-spin stretching methods amenable to recombinant spider aciniform silk Biomacromolecules 17:2737-2746 [PubMed] [Article] Sarker, M., Orrell, K.E., Xu, L., Tremblay, M-L., Bak, J.J., Liu, X-Q. and Rainey, J.K., (2016) Tracking transitions in spider wrapping silk conformation and dynamics by 19F nuclear magnetic resonance spectroscopy Biochemistry 55:3048–3059 [PubMed] [Article] Read, J., Clancy, E.K., Sarker, M., de Antueno, R., Langelaan, D.N., Parmar, H.B., Shin, K., Rainey, J.K. and Duncan, R., (2015) Reovirus FAST proteins drive pore formation and syncytiogenesis using a novel helix-loop-helix fusion-inducing lipid packing sensor PLoS Pathog 11(6):e1004962 [PubMed] [Article] Sarker, M., Fraser, R.E., Lumsden, M.D., Anderson, D.J. and Rainey, J.K., (2015) Characterization of variant soft nanoparticle structure and morphology in solution by NMR spectroscopy. J Phys Chem C 119:7461-7471 [Article] Key, T., Sarker, M., de Antueno, R., Rainey, J.K. and Duncan, R., (2015) The p10 FAST protein fusion peptide functions as a cystine noose to induce cholesterol-dependent liposome fusion without liposome tubulation. Biochim Biophys Acta - Biomembranes 1848:408-416 [PubMed] [Article] Eftaiha, A.F., Tremblay, M-L., Rainey, J.K. and Paige, M.F, (2015) The effect of perfluorooctadecanoic acid on a model phosphatidylcholine-peptide pulmonary lung surfactant mixture. J. Fluorine Chem. 177:55-61 [Article] Clattenburg, L., Wigerius, M., Qi, J., Rainey, J.K., Rourke, J.L., Muruganandan, S., Sinal, C.J. and Fawcett, J.P., (2015) NOS1AP functionally associates with YAP to regulate Hippo signaling Mol. Cell. Biol. 35:2265-2277 [PubMed] [Article] Tremblay, M-L., Xu, L., Lefèvre, T., Sarker, M., Orrell, K.E., Leclerc, J., Meng, Q., Pezolet, M., Auger, M., Liu, X-Q. and Rainey, J.K., (2015) Spider wrapping silk fiber architecture arising from its modular soluble protein precursor. Sci. Rep. 5:11502 [PubMed] [Article] Pandey, A., Sarker, M., Liu, X-Q. and Rainey, J.K., (2014) Small expression tags enhance bacterial expression of the first three transmembrane segments of the apelin receptor. Biochem Cell Biol 92:269-278 [PubMed] [Article] Chapman, N.A., Dupré, D.J. and Rainey, J.K., (2014) The apelin receptor: Physiology, pathology, cell signalling, and ligand modulation of a peptide-activated class A GPCR. Biochem Cell Biol 92:431-440 [PubMed] [Article] Ramu, T., Prasad, M., Connors, E., Mishra, A., Thomassin, J-L., Leblanc, J., Rainey, J.K. and Thomas, N., (2013) A novel C-terminal region within the multicargo type III secretion chaperone CesT contributes to effector secretion. J. Bacteriol. 195:740-756 [PubMed] Xu, L., Tremblay, M-L., Orrell, K.E., Leclerc, J., Meng, Q., Liu, X-Q. and Rainey, J.K., (2013) Nanoparticle self-assembly by a highly stable recombinant spider wrapping silk protein subunit. FEBS Lett 587:3273-3280 [PubMed] [Article] Langelaan, D.N., Reddy, T., Banks, A.W., Dellaire, G., Dupré, D. and Rainey, J.K., (2013) Structural features of the apelin receptor N-terminal tail and first transmembrane segment implicated in ligand binding and receptor trafficking. Biochimica et Biophysica Acta - Biomembranes 1828:1471-1483 [PubMed] [Article] Kehoe, S., Tremblay, M-L., Coughlan, A., Towler, M.R., Rainey, J.K., Abraham, R.J. and Boyd, D., (2013) Preliminary Investigation of the Dissolution Behavior, Cytocompatibility, Effects of Fibrinogen Conformation and Platelet Activation for Radiopaque Embolic Particles. J. Funct. Biomater. 4:89-113 [Article] Shin, K., Pandey, A., Liu, X-Q., Anini, Y. and Rainey, J.K., (2013) Preferential apelin-13 production by the proprotein convertase PCSK3 is implicated in obesity. FEBS Open Bio. 3:328-333 [PubMed] [Article]

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