JOCKUSCH, Rebecca - University of Toronto

研究领域

Our research is of both fundamental and applied interest. Understanding of the forces controlling protein structure and dynamics is essential to understand and manipulate biological systems. We are particularly interested in determining the role of non-covalent interactions, such as protein-water interactions, on protein structure and stability. From an applications perspective, mass spectrometry is now routinely used to analyze biological systems. While in some cases solution-phase structural properties appear to be retained in the gas phase, other studies indicate little or no correlation. Thus, although gas-phase analysis shows great promise in areas like drug binding assays, the unresolved relationship between mass spectrometry and solution-phase studies greatly restricts its utility. Our studies are designed to bridge between solution and the gas phase.

近期论文

查看导师最新文章（温馨提示：请注意重名现象，建议点开原文通过作者单位确认）

F.O. Talbot, S.V. Sciuto, and R.A. Jockusch, “Fluorescence imaging for visualization of the ion cloud in a quadrupole ion trap mass spectrometer”, Journal of The American Society for Mass Spectrometry, 24(12),1823-1832 (2013). DOI: 10.1007/s13361-013-0742-6. M.F. Czar and R.A. Jockusch, “Understanding Photophysical Effects of Cucurbituril Encapsulation: A Model Study with Acridine Orange in the Gas Phase”, ChemPhysChem, 14(6), 1138-1148 (2013). Designated as Very Important Paper (Inside Cover). DOI: 10.1002/cphc.201201008. H. Yao and R.A. Jockusch, “Fluorescence and electronic action spectroscopy of mass-selected gas-phase fluorescein, 2′,7′-dichlorofluorescein, and 2′,7′-difluorofluorescein ions”, Journal of Physical Chemistry A, 117(6), 1351-1359 (2013). DOI: 10.1021/jp309767f. B.L. Heath and R.A. Jockusch, “Ligand Migration in the Gaseous Insulin-CB7 Complex-A Cautionary Tale About the Use of ECD-MS for Ligand Binding Site Determination”, Journal of The American Society for Mass Spectrometry, 23(11), 1911-1920 (2012). DOI: 10.1007/s13361-012-0470-3. A.M. Nagy, F.O. Talbot, M.F. Czar, and R.A. Jockusch, “Fluorescence Lifetimes of Rhodamine Dyes in Vacuo“, Journal of Photochemistry and Photobiology A, 244, 47-53 (2012). DOI: 10.1016/j.jphotochem.2012.06.017. J. Pan, B.L. Heath, R.A. Jockusch, and L. Konermann, “Structural Interrogation of Electrosprayed Peptide Ions by Gas-Phase H/D Exchange and Electron Capture Dissociation Mass Spectrometry”, Analytical chemistry, 84(1), 373-378 (2011). DOI: 10.1021/ac202730d. H. Yao, J.D. Steill, J. Oomens, and R.A. Jockusch, “Infrared Multiple Photon Dissociation Action Spectroscopy and Computational Studies of Mass-Selected Gas-Phase Fluorescein and 2′,7′-Dichlorofluorescein Ions”, J. Phys. Chem. A, 115(34), 9739-9747 (2011). DOI: 10.1021/jp201946a. M.W. Forbes, A.M. Nagy, and R.A. Jockusch, “Photofragmentation of and electron photodetachment from a GFP model chromophore in a quadrupole ion trap”, International Journal of Mass Spectrometry, 308(2-3), 155-166 (2011). DOI: 10.1016/j.ijms.2011.08.016. V.A. Braz, M.D. Barkley, R.A. Jockusch, and P.L. Wintrode, “Efavirenz Binding Site in HIV-1 Reverse Transcriptase Monomers”, Biochemistry, 49(49), 10565-10573 (2010). DOI: 10.1021/bi101480z.. S.K. Sagoo and R.A. Jockusch, “The fluorescence properties of cationic rhodamine B in the gas phase”, Journal of Photochemistry and Photobiology A: Chemistry, 220(2-3), 173-178 (2011). DOI: 16/j.jphotochem.2011.04.008. F.O. Talbot, A. Rullo, H. Yao, and R.A. Jockusch, “Fluorescence Resonance Energy Transfer in Gaseous, Mass-Selected Polyproline Peptides”, Journal of the American Chemical Society, 132(45), 16156-16164 (2010). DOI:10.1021/ja1067405. P.D. McQueen, S. Sagoo, H. Yao, and R.A. Jockusch, “On the Intrinsic Photophysics of Fluorescein”, Angewandte Chemie International Edition, 49(48), 9193-9196 (2010). DOI: 10.1002/anie.201004366. M.W. Forbes and R.A. Jockusch, “Gas-Phase Fluorescence Excitation and Emission Spectroscopy of Three Xanthene Dyes (Rhodamine 575, Rhodamine 590 and Rhodamine 6G) in a Quadrupole Ion Trap Mass Spectrometer”,Journal of The American Society for Mass Spectrometry, 22(1), 93-109 (2011). DOI: 10.1007/s13361-010-0017-4. Q. Bian, M.W. Forbes, F.O. Talbot, and R.A. Jockusch, “Gas-phase fluorescence excitation and emission spectroscopy of mass-selected trapped molecular ions”, Physical Chemistry Chemical Physics, 12(11), 2590-2598 (2010). DOI: 10.1039/b921076h. M.W. Forbes and R.A. Jockusch, “Deactivation Pathways of an Isolated Green Fluorescent Protein Model Chromophore Studied by Electronic Action Spectroscopy”, Journal of the American Chemical Society, 131(47), 17038-17039 (2009). DOI: 10.1021/ja9066404. M.W. Forbes, F.O. Talbot, and R.A. Jockusch, “The Spectroscopy of Ions Stored in Trapping Mass Spectrometers” in Practical Aspects of Trapped Ion Mass Spectrometry: Applications of Ion Trapping Devices 5, 239-290 (2009). M.W. Forbes, M.F. Bush, N.C. Polfer, J. Oomens, R.C. Dunbar, E.R. Williams, and R.A. Jockusch, “Infrared spectroscopy of arginine cation complexes: Direct observation of gas-phase zwitterions”, Journal of Physical Chemistry A, 111(46), 11759-11770 (2007). DOI: 10.1021/jp074859f. M.F. Bush, M.W. Forbes, R.A. Jockusch, J. Oomens, N.C. Polfer, R.J. Saykally, and E.R. Williams, “Infrared spectroscopy of cationized lysine and epsilon-N-methyllysine in the gas phase: Effects of alkali-metal ion size and proton affinity on zwitterion stability”, Journal of Physical Chemistry A, 111(32), 7753-7760 (2007). DOI: 10.1021/jp071902q.