个人简介

Dipl. Phys., University of Heidelberg (Germany) PhD, Swiss Federel Institute of Technology at Lausanne (EPFL, Switzerland), Post-doc., Stanford University (USA)

研究领域

Biophysical Fluorescence

BIOPHYSICAL FLUORESCENCE The research in my group is directed towards biophysics with an emphasis on biophysical fluorescence. This highly interdisciplinary domain requires the interaction of chemists, physicists, and biologists. Only a concerted effort of these three groups will allow us to tackle the problems in the life sciences. The close proximity of departments of biology, biochemistry, chemistry, and physics at the National University of Singapore on one side and the research institutes (RIs) on the other side, allows us to develop physical and chemical methods for the study of biological questions on one hand and to apply these methods to the frontier in biology on the other hand. Our interests lie accordingly in the different areas that interact freely to advance this research field. Construction and development of new optical tools. Optical Spectroscopy is one of the most sensitive tools available in the life sciences. Proteins can be studied not only in ensembles but as well on the single molecule level. Besides using well established methods in our group (e.g. Fluorescence Correlation Spectroscopy, Fluorescence Resonance Energy Transfer), we also plan to develop new spectroscopy and microscopy tools and new mathematical procedures to study proteins on a single molecule level in vitro and in vivo. Study of selected proteins and protein complexes on a single molecule level and in living cells. In collaboration with the Department of Biological Sciences, the Department of Microbiology and the RIs we will study the properties of selected proteins and peptides to elucidate their function on a molecular level. At the moment we concentrate on either antimicrobial peptides and their interaction with bacterial membranes, or on the study of transmembrane proteins (G-protein coupled receptors, growth factors) and their structure, function and interactions. One of the most interesting questions in biology is the relationship between the structure and function of proteins. With the fluorescence tools developed in our group we hope to shed some light on this question by in vitro experiments. In a complementary approach we will study the proteins in living cells because proteins are in many cases very sensitive to their environment and only when studied under physiological conditions can we determine their exact function.

近期论文

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Rashid, R.; Beyer, S.; Blocki, A.; Le Visage, C.; Trau, D.; Wohland, T.; Raghunath, M. Mitochondrial Routing of Glucose and Sucrose Polymers After Pinocytotic Uptake: Avenues For Drug Delivery, Biomacromolecules (2014) Jun 9;15(6):2119-27 Rashid, R.; Lim, N.S.J.; Chee, S.M.L.; Png, S.N.; Wohland, T.; Raghunath, M. Novel Use for Polyvinylpyrrolidone as a Macromolecular Crowder for Enhanced Extracellular Matrix Deposition and Cell Proliferation, Tissue Engineering Part C (2014) May 2. (Epub) Machan, R.; Wohland, T. Recent Applications of Fluorescence Correlation Spectroscopy in Live Systems, FEBS Letters 588 (2014) 3571–3584. Singh, A.P.; Wohland, T. Applications of imaging fluorescence correlation spectroscopy, Curr. Op. Chem. Biol. (2014) 20:29-35. Guo, S.M.; Bag, N.; Mishra, A.;Wohland, T.; Bathe, M. Bayesian Total Internal Reflection Fluorescence Correlation Spectroscopy Reveals hIAPP-Induced Plasma Membrane Domain Organization in Live Cells, Biophy. J. 106 (2014) 190–200 Bag, N.; Yap, D.H.X.; Wohland, T. Temperature dependence of diffusion in model and live cell membranes characterized by imaging fluorescence correlation spectroscopy, BBA Biomembranes 1838 (2014) 802–813. Krieger, J.W.; Singh, A.P.; Garbe, C.S.; Wohland, T.; Langowski, J. Dual-Color Fluorescence Cross-Correlation Spectroscopy on a Single Plane Illumination Microscope (SPIM-FCCS), Optics Express 22(3) (2014) 2358-2375. Bag, N.; Wohland, T. Imaging Fluorescence Fluctuation Spectroscopy: New Tools for Quantitative Bioimaging. Annu. Rev. Phys. Chem. 65 (2014) 225–48. Bag, N; Ali, A; Chauhan, VS; Wohland, T; Mishra, A. Membrane destabilization by monomeric hIAPP observed by imaging fluorescence correlation spectroscopy, Chem Commun (Camb). 49 (80) (2013) 9155-7. Sankaran, J.; Bag, N.; Kraut, R.S.; Wohland, T. Accuracy and precision in camera-based fluorescence correlation spectroscopy measurements, Anal. Chem. 85 (8) (2013) 3948-54. Singh, A.P.; Krieger, J.W.; Buchholz, J.; Charbon, E.; Langowski, J.; Wohland, T. The performance of 2D array detectors for light sheet based fluorescence correlation spectroscopy, Optics Express, 21(7) (2013) 8652-8668. Supplementary material Lauterbach, T.; Manna, M.; Ruhnow, M.; Wisantoso, Y.; Wang, Y.; Matysik, A.; Oglecka, K.; Mu, Y.; Geifman-Shochat, S.; Wohland, T.; Kraut, R. Weak Glycolipid Binding of a Microdomain-Tracer Peptide Correlates with Aggregation and Slow Diffusion on Cell Membranes, Plos ONE 7 (12) (2012), e51222. Lam, C.S.; Mistri, T.K.; Foo, Y.H.; Sudhaharan, T.; Gan, H.T.; Rodda, D.; Lim, L.H.; Chou, C.; Robson, P.; Wohland, T.; Ahmed, S. DNA dependent Oct4-Sox2 interaction and diffusion properties characteristics of the pluripotent cell state revealed by fluorescence spectroscopy, Biochem J., 488 (2012) 21-33. Bag, N.; Sankaran, J.; Paul, A.; Kraut, R.; Wohland, T. Calibration and Limits of Camera-Based Fluorescence Correlation Spectroscopy: A Supported Lipid Bilayer Study, Chemphyschem 13(11) (2012) 2784-94. Kay, J.G.; Koivusalo, M.; Ma, X.; Wohland, T.; Grinstein, S. Phosphatidylserine dynamics in cellular membranes, Mol Bio Cell, 23 (11) (2012) 2198-2212. Guo, S-M.; He, J.; Monnier, N.; Sun, G.; Wohland, T.; Bathe, M. A Bayesian approach to the analysis of fluorescence correlation spectroscopy data II: Application to simulated and in vitro data, Anal Chem, 84 (9) (2012) 3880-3888. Foo, Y.H.; Naredi-Rainer, N.; Lamb, D.C.; Ahmed, S.; Wohland, T. Factors Affecting the Quantification of Biomolecular Interactions by Fluorescence Cross-Correlation Spectroscopy, Biophys J, 102 (2012) 1174-1183. Korzh, V.; Wohland, T. Analysis of properties of single molecules in vivo or... why small fish is better than empty dish, Russian Journal of Developmental Biology 43 (2) (2012) 67-76. Kraut, R.; Bag, N.; Wohland, T. Fluorescence Correlation Methods for Imaging Cellular Behavior of Sphingolipid-Interacting Probes, Methods in Cell Biology 108 (2012) 395-427. Ma, X.; Ahmed, S.; Wohland, T. EGFR activation monitored by SW-FCCS in live cells, Front Biosci (Elite Ed), 3(2011) 22-32.