Rafferty, John - University of Sheffield - Department of Molecular Biology and Biotechnology

个人简介

Career History 2005 - present: Reader in Structural Biology, Mol.Biol.&Biotech.Dept, Sheffield Univ 1999 - 2004: Royal Society Olga Kennard Fellow, Mol.Biol.&Biotech.Dept, Sheffield Univ 1996 - 1999: BBSRC David Phillips Research Fellow, Mol.Biol.&Biotech.Dept, Sheffield Univ. 1992 - 1996: PDRA, Mol.Biol. & Biotech. Dept, Sheffield Univ. 1990 - 1991: PDRA, Biochem. & Mol.Biol. Dept, Leeds Univ. 1989 - 1990: PDRA, Biophysics Dept, Leeds Univ. 1986 - 1990: PhD, Biophysics Dept, Leeds Univ.

研究领域

Structural study of proteins and DNA primarily by X-ray crystallography and electron microscopy to gain 3D insights of biological macromolecules and their assemblies. Structure and function relationships.

近期论文

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AlMalki FA, Flemming CS, Zhang J, Feng M, Sedelnikova SE, Ceska T, Rafferty JB, Sayers JR & Artymiuk PA (2016) Direct observation of DNA threading in flap endonuclease complexes. Nature Structural and Molecular Biology, 23, 640-646. View this article in WRRO Khavnekar S, Dantu SC, Sedelnikova S, Ayora S, Rafferty J & Kale A (2016) Structural insights into dynamics of RecU–HJ complex formation elucidates key role of NTR and stalk region toward formation of reactive state. Nucleic Acids Research. View this article in WRRO Phansopa C, Roy S, Rafferty JB, Douglas CW, Pandhal J, Wright PC, Kelly DJ & Stafford GP (2014) Structural and functional characterization of NanU, a novel high-affinity sialic acid-inducible binding protein of oral and gut-dwelling Bacteroidetes species.. Biochem J, 458(3), 499-511. View this article in WRRO Green V, Curtis FA, Sedelnikova S, Rafferty JB & Sharples GJ (2013) Mutants of phage bIL67 RuvC with enhanced Holliday junction binding selectivity and resolution symmetry.. Mol Microbiol, 89(6), 1240-1258. View this article in WRRO Salmon RC, Cliff MJ, Rafferty JB & Kelly DJ (2013) The CouPSTU and TarPQM transporters in Rhodopseudomonas palustris: redundant, promiscuous uptake systems for lignin-derived aromatic substrates.. PLoS One, 8(3), e59844. View this article in WRRO Afonso JP, Chintakayala K, Suwannachart C, Sedelnikova S, Giles K, Hoyes JB, Soultanas P, Rafferty JB & Oldham NJ (2013) Insights into the structure and assembly of the Bacillus subtilis clamp-loader complex and its interaction with the replicative helicase.. Nucleic Acids Res, 41(9), 5115-5126. View this article in WRRO Abd Aziz AG, Sedelnikova SE, Ruzheinikov SN, Thorpe S, Mohamed R, Nathan S, Rafferty JB, Baker PJ & Rice DW (2012) Crystallization and preliminary X-ray analysis of the receiver domain of a putative response regulator, BPSL0128, from Burkholderia pseudomallei.. Acta Crystallogr Sect F Struct Biol Cryst Commun, 68(Pt 8), 917-922. Canas C, Carrasco B, Garcia-Tirado E, Rafferty JB, Alonso JC & Ayora S (2011) The Stalk Region of the RecU Resolvase Is Essential for Holliday Junction Recognition and Distortion. J MOL BIOL, 410(1), 39-49. Kale A, Phansopa C, Suwannachart C, Craven CJ, Rafferty JB & Kelly DJ (2011) The virulence factor PEB4 (Cj0596) and the periplasmic protein Cj1289 are two structurally related SurA-like chaperones in the human pathogen Campylobacter jejuni.. J Biol Chem, 286(24), 21254-21265.