Brestovac, Brian - Curtin University - School of Biomedical Sciences

研究领域

Developing a flow-cytometry method for cervical cancer screening. The old cytology method lacks sensitivity, is labour intensive and expensive. By using the flow-cytometer instrument these problems should be resolved. This is particularly important due to the use of HPV vaccine which should reduce the number of positive samples, making the old method very expensive per life saved. The flow-cytometry method should deliver a superior service at a lower cost. I am also involved in investigating the anti-cancer properties of the plant Sarcostemma veminale. Preliminary findings with cancer cell lines are encouraging and this may result in a therapy which destroys cancer without damaging normal healthy tissue. The Sarcostemma veminale plant extract has been fractionated and results indicate that one of the fractions contains the active compound. Further testing will continue to isolate the active compound. The immune modulation properties of Sarcostemma veminale was also investigated by exposing macrophages to the plant extract and results show that it induces a pro-inflammatory and hence an anti-cancer responce. The results of this study was presented to the ASMR conference 2012 by my honours student Ozzie Coghlan who won a gold award for the presentation. I am interested in immuno-genetic association with disease. I have investigated the association of the Chemokine receptor deletion (CCR5) with primary dengue fever. I have also investigated the association between Killer Immunoglobulin-like Receptors and cervical cancer. Much of my earlier work focused on Human papillomavirus and cervical cancer. In my work I determined that HPV type 53 was not oncogenic. I also developed a multiplex nested PCR method to detect High-Risk HPV types.

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Brestovac, B., O. Coghlan, C. Jackaman, D. J. Nelson, and D. E. Townsend. 2015.“Sarcostemma viminale activates macrophages to a pro-inflammatory phenotype.”Comparative Clinical Pathology 24 (4): 817-826. Brestovac, B., M. Wong, P. Costantino, and D. M. Groth. 2014.“A Rapid DNA Extraction Method Suitable for Human Papillomavirus Detection.”Journal of Medical Virology 86 (4): 653-657. Brestovac, B., L. A. Halicki, R. P. Harris, I. Sampson, D. Speers, C. D. Mamotte, and D. T. Williams. 2014.“Primary acute dengue and the deletion in chemokine receptor 5 (CCR5[delta]32).”Microbes and Infection 16: 215-521. Brestovac, B., D. E. Townsend, J. Snook, G. Ellison, and A. Phillips. 2013.“Sarcostemma viminale: a potential anticancer therapy.”Comparative Clinical Pathology Oct 2013. Brestovac, B., M. E. Wong, R. Tjendera, P. Costantino, C. D. Mamotte, and C. S. Witt. 2013.“Human papillomavirus, high-grade intraepithelial neoplasia and killer immunoglogulin-like receptors: a Western Australian cohort study.”Infectious Agents and Cancer 8: 1-5. Brestovac, B., G. B. Harnett, D. W. Smith, F. Frost, and G. Shellam. 2005.“Multiplex nested PCR (MNP) assay for the detection of 15 high risk genotypes of human papillomavirus.”Journal of Clinical Virology 33: 116-122. Brestovac, B., G. B. Harnett, D. W. Smith, G. Shellam, and F. Frost. 2005.“Human Papillomavirus Genotypes and Their Association With Cervical Neoplasia in a Cohort of Western Australian Women.”Journal of Medical Virology 76: 106-110. Starac, D., B. Brestovac, G. Sterrett, D. W. Smith, and F. Frost. 2005.“Can HPV DNA testing on FNA material determine anogenital origin in metastatic squamous cell carcinoma?.”Pathology 37 (3): 197-203. Dimech, W., D. S. Bowden, B. Brestovac, K. Byron, G. James, D. Jardine, T. Sloots, and E. M. Dax. 2004.“Validation of assembled nucleic acid-based tests in diagnostic microbiology laboratories.”Pathology 36 (1): 45-50. Plunkett, M. K., B. Brestovac, J. E. Thompson, G. Sterrett, P. R. Fillon, D. W. Smith, and F. Frost. 2003.“The value of HPV DNA typing in the distinction between adenocarcinoma of endocervical and endometrial origin.”Pathology 35 (5): 397-401. Sayer, D., R. Whidborne, B. Brestovac, F. Trimboli, C. Witt, and F. Christiansen. 2001.“HLA-DRB1 DNA sequencing based typing: An approach suitable for high throughput typing including unrelated bone marrow registry donors.”Tissue Antigens 57 (1): 46-54.