个人简介

1987-1993 Study of Physics and Biochemistry, University Bayreuth 1993 Diploma thesis "2D-NMR investigations at the acceptor arm of the tRNAAla of E.Coli" (under the supervision of Prof. Dr. Mathias Sprinzl and Prof. Dr. Marcus Schwoerer). 1994-1998 PhD thesis "Development of novel NMR spectroscopic Methods for the analysis of the Constitution and Conformation of Biomacromolecules, Natural Products and Metall-organic Compounds", Institut für Organische Chemie, Universität Frankfurt/M. (supervisor: Prof. Dr. Christian Griesinger) 1998-1999 Postdoctoral research assistant at the Massachusetts Institute of Technology (M.I.T.), Boston, USA (supervisor: Prof. Dr. Robert G. Griffin) 1999-2002 Group Leader according to the Emmy-Noether-Research program of the DFG, Technische Universität München 2003-2010 Group Leader "Solid-State NMR" at the Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP) in Berlin-Buch 2004 Appointment for life to a C3 professorship at the Charité Universitätsmedizin in Berlin 2005 Affiliation with the Center for Biophysics and Bioinformatics (BPI) of the Humboldt-Universität Berlin. Cooption at the Faculty of Mathematics and Natural Sciences of the HU Berlin 2007- Coordinator of the Leibniz Graduate School Molecular Biophysics, Berlin 2010- W2 Appointment at the Technische Universität München 2010- Affiliation as a group leader at the Helmholtz Center München, German Research Center for Environmental Health

研究领域

Organic Chemistry and Biochemistry

Prof. Reif’s (b. 1968) group examines the structure of membrane proteins and amyloid fibrils using MAS (magic angle spinning) solid-state NMR spectroscopy. Research is focused on aggregates of the Alzheimer’s beta amyloid peptide. NMR experiments help to determine the binding site of small molecules. This work is highly significant in terms of diagnosis and treatment. Methodical new approaches are developed to gather information on the structure and dynamics of uniform isotope-labeled proteins in the solid phase.

近期论文

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Jacso, T., Bardiaux, B., Broecker, J., Fiedler, S., Baerwinkel, T., Mainz, A., Fink, U., Vargas, C., Oschkinat, H., Keller, S., Reif, B., The Mechanism of Denaturation and the Unfolded State of the α Helical Membrane-Associated Protein Mistic, J. Am. Chem. Soc. 135, 1884-18891 (2013). Zinkevich, T., Chevelkov, V., Reif, B., Saalwächter, K., Krushelnitzky, A., Multi-frequency 15N solid-state NMR relaxation analysis of protein dynamics validates motional models and offers surprising sensitivity to slow motions with very high order parameters, J. Biomol. NMR 57, 219-235 (2013). Asami, S., Reif, B., Proton-detected solid-state NMR at aliphatic sites: Applications to Crystalline Systems, Acc. Chem. Res. 46 (9), 2089-2097 (2013). Lopez del Amo, J.M., Schneider, D., Lange, A., Reif, B., Field Cryogenic solid-state NMR studies of fibrils of the Alzheimer's disease amyloid-β peptide: Perspectives for DNP, J. Biomol. NMR 56, 359-363 (2013). Mainz, A., Religa, T., Sprangers, R., Linser, R., Kay, L.E., Reif, B., Proton detection of MDa sized protein complexes in solution by MAS solid-state NMR, Angewandte Chemie Int. Edt. 52, 8746-8751 (2013). Agarwal, V., Linser, R., Dasari, M., Fink, U., Lopez del Amo, J.M., Reif, B., Hydrogen bonding involving side chain exchangeable groups stabilizes amyloid quarternary structure, Phys. Chem. Chem. Phys. 15, 12551-12557 (2013). Ravera, E., Parigi, G., Mainz, A., Religa, T. L., Reif, B., Luchinat, C., Experimental Determination of Microsecond Reorientation Correlation Times in Protein Solutions, J. Phys. Chem. B 117, 3548-3553 (2013). Asami, S., Rakwalska-Bange, M., Carlomagno, T., Reif, B., Protein/RNA interfaces probed by 1H detected MAS solid-state NMR, Angewandte Chemie Int. Edt. Engl. 52 2345-2349 (2013). Asami, S., Szekely, K., Schanda, P., Meier, B.H., Reif, B., Optimal degree of protonation for 1H detection of aliphatic sites as a function of the MAS frequency, J. Biomol. NMR 54 155-168 (2012). Lopez del Amo, J.M., Dasari, M., Fink, U., Grelle, G., Wanker, E.E., Bieschke, J., Reif, B., Structural analysis of Alzheimer disease beta-amyloid oligomers induced by the green tea compound epigallocatechin-3-gallate (EGCG), J. Mol. Biol. 421 517-524 (2012). Lopez del Amo, J.M., Schmidt, M., Fink, U., Dasari, M., Fändrich, M., Reif, B., An asymmetric dimer can be the basic subunit in Alzheimer's disease β-amyloid fibrils, Angewandte Chemie Int. Edt. Engl. 51 6136-6139 (2012). Jacso, T., Schneider, E., Rupp, B., Reif, B., Substrate transport activation is mediated through the second periplasmic loop P2 of MalF in the maltose transport complex of Escherichia coli, J. Biol. Chem. 287 17040-17049 (2012). Reif, B., Ultra-high resolution in MAS solid-state NMR of perdeuterated proteins: Implications for Structure and Dynamcis, J. Magn. Reson. 216 1-12 (2012). Kaden, D., Munter, L.-M., Reif B., Multhaup, G., The Amyloid Precursor Protein and its homologues: Structural and functional aspects of native and pathogenic oligomerization, Eur. J. Cell Biol. 91 234-239 (2012). Asami, S., Reif, B., Assignment strategies for aliphatic protons in the solid-state in randomly protonated proteins, J. Biomol. NMR 52 31-39 (2012). Mainz, A., Bardiaux, B., Kuppler, F., Multhaup, G., Felli, I., Pierattelli, R., Reif, B., Strcutural and mechanistic implications of metal-binding in the small heat shock protein αB-crystallin, J. Biol. Chem. 287 1128-1138 (2012). Bieschke, J., Herbst, M., Wiglenda, T., Friedrich, R., Boeddrich, A., Schiele, F., Kleckers, D., Lopez del Amo, J.M., Gruening, B., Wang, Q., Schmidt, M., Lurz, R., Anwyl, R., Schnoegl, S., Fändrich, M., Frank, R., Reif, B., Guenther, S., Walsh, D., Wanker, E.E., Small-molecule conversion of toxic oligomers to non-toxic β-sheet-rich amyloid fibrils, Nature Chem. Biol. 8 93-101 (2012). Jacso, T., Franks, T.W., Rose, H.M., Fink, U., Broecker, J., Keller, S., Oschkinat, H., Reif, B., Characterization of Membrane Proteins in Isolated Native Cellular Membranes by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy without Purification and Reconstitution, Angewandte Chemie Int. Edt. 51 432-435 (2012). Lalli, D., Schanda, P., Chowdhury, A., Retel, J., Hiller, M., Agarwal, V., Reif, B., van Rossum, B., Akbey, U., Oschkinat, H., Three-Dimensional Deuterium-Carbon Correlation for High-Resolution Solid-State MAS NMR Spectroscopy of Large Proteins, J. Biomol. NMR. 51 477-485 (2011). Bertini, I., Luchinat, C., Parigi, G., Ravera, E., Reif, B., Turano, P., Solid-state NMR of proteins sedimented by ultracentrifugation, Proc. Natl. Acad. Sci. U.S.A. 108 10396-10399 (2011).