个人简介
吴坚平，男，1973年2月于杭州出生，祖籍江苏无锡。教授，工学博士，1991年进入浙江大学化工系生物化工专业，1995年本科毕业获得生物化工学士学位，同年免试进入浙江大学化工系生物化工专业攻读硕士学位，1996年转为硕博一贯制博士研究生攻读生物化工专业博士学位，2000年按期毕业后留校，在化工工程与生物工程学院生物工程研究所任教，从事教学和科研工作，
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Fei, H; Xu, G; Wu, JP*; Yang, LR. Improving the acetaldehyde tolerance of DERA(SEP) by enhancing the rigidity of its protein structure. Journal of Molecular Catalysis B-Enzymatic, 2015-06, 116: 148-152.
Xu, G; Lan, SQ; Fu, SM; Wu, JP*; Yang, LR. Highly efficient dynamic kinetic resolution of benzylic amines with palladium catalysts on oxidized multiwalled carbon nanotubes for racemization. Tetrahedron Letters, 2015-05, 56(21):2714-2719.
Wu, JP; Li, M; Zhou, Y; Yang, LR; Xu, G*. Introducing a salt bridge into the lipase of Stenotrophomonasmaltophilia results in a very large increase in thermal stability. Biotechnology Letters, 2015-02, 37(2):403-407.
Wu, JP; Meng, X; Wang, L; Xu, G*; Yang, LR. Highly efficient dynamic kinetic resolution of secondary aromatic alcohols at low temperature using a low-cost sulfonatedsepiolite as racemization catalyst. Tetrahedron Letters, 2014-09, 55(37):5129-5132.
Li, M; Yang, LR; Xu, G; Wu, JP*. Screening, purification and characterization of a novel cold-active and organic solvent-tolerant lipase from Stenotrophomonasmaltophilia CGMCC 4254. Bioresource Technology, 2013-11, 148:114-120.
Xu, G; Wang, L; Chen, YJ; Cheng, YM; Wu, JP*; Yang, LR. Highly efficient dynamic kinetic resolution of secondary aromatic alcohols using a low-cost solid super acid as a racemization catalyst. Tetrahedron Letters, 2013-09, 54(37):5026-5030.
Lin, CJ; Yang, LR; Xu, G; Wu, JP*. Enhancement of HaloacetateDehalogenase Production by Strain Mutation and Condition Optimization. Biotechnology and Bioprocess Engineering, 2011-10, 16(5):923-929.
Lin, CJ; Yang, LR; Xu, G; Wu, JP*. Biodegradation and metabolic pathway of beta-chlorinated aliphatic acid in Bacillus sp. CGMCC no. 4196. Applied Microbiology and Biotechnology, 2011-04, 90(2):689-696.
Fei, H; Xu, G; Wu, JP*; Yang, LR. An improved flurogenic probe for high-throughput screening of 2-deoxyribose aldolases. Biochemical and Biophysical Research Communications, 2015-05, 460(3):826-830.
Xu, G; Meng, X; Xu, LJ; Guo, L; Wu, JP*; Yang, LR. Modification and simulation of Rhizomucormiehei lipase: the influence of surficial electrostatic interaction on enantioselectivity. Biotechnology Letters, 2015-04, 37(4):871-880.
Dai, XT; Wu, JP*; Meng, X; Xu, G*; Yang, LR. Preparation of (R)-1-Aminoindan by Dynamic Kinetic Resolution. Chinese Journal of Organic Chemistry, 2014-05, 34(5):933-937.
Fei, H; Xu, G; Wu, JP*; Yang, LR. Improvement of the thermal stability and aldehyde tolerance of deoxyriboaldolase via immobilization on nano-magnet material. Journal of Molecular Catalysis B-Enzymatic, 2014-03, 101:87-91.
Pei, XL; Yang, LR; Xu, G; Wang, QY; Wu, JP*. Discovery of a new Fe-type nitrile hydratase efficiently hydrating aliphatic and aromatic nitriles by genome mining. Journal of Molecular Catalysis B-Enzymatic, 2014-01, 99:26-33.
Li, M; Yang, LR; Xu, G; Wu, JP*. Identification of organic solvent-tolerant lipases from organic solvent-sensitive microorganisms. Journal of Molecular Catalysis B-Enzymatic, 2014-01, 99:96-101.
Peix XL; Zhang, HY; Meng, LJ; Xu, G; Yang, LR; Wu, JP*. Efficient cloning and expression of a thermostable nitrile hydratase in Escherichia coli using an auto-induction fed-batch strategy. Process Biochemistry, 2013-12, 48(12):1921-1927.
Tao, WH; Yang, LR; Xu, G; Qiao, YB; Wu, JP*. Immobilization of Lipase on Zirconium Nucleotide and Its Catalytic Performance. Chinese journal of catalysis, 2011-04, 32(4):630-636.
Mu Li; Li-Rong Yang; Gang Xu; Jian-Ping Wu*. Cloning and characterization of a novel lipase from Stenotrophomonasmaltophilia GS11: The first member of a new bacterial lipase familyXVI. Journal of Biotechnology, 2016, 228: 30-36.
Mu Li; Li-Rong Yang; Gang Xu; Jian-Ping Wu*. Highly diastereoselective acylation of l-menthol by a lipase from Stenotrophomonas maltophilia CGMCC 4254. Biochemical Engineering Journal, 2016, 109: 81-87.
Pei, XL; Yang, ZF; Wang, AM ; Yang, LR; Wu, JP. Identification and functional analysis of the activator gene involved in the biosynthesis of Co-type nitrile hydratase from Aurantimonas manganoxydans, Journal of Biotechnology, 2017, 251: 38-46.
Pei, XL; Wang, QY; Meng, LJ ; Li, J; Yang, ZF; Yin, XP ; Yang, LR; Chen, SY ; Wu, JP*. Chaperones-assisted soluble expression and maturation of recombinant Co-type nitrile hydratase in Escherichia coli to avoid the need for a low induction temperature, Journal of Biotechnology，203：9-16.
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