Undergraduate students in an environmental chemistry laboratory course were taught QuEChERS (quick, easy, cheap, effective, rugged, and safe), a sample preparation procedure that is commonly used in pesticide laboratories involving an acetonitrile salt-out extraction of fresh produce samples followed by solid-phase dispersive cleanup using a combination of sorbents. The cleaned extract was solvent exchanged into toluene and analyzed for pesticides by capillary gas chromatography–mass spectrometry in selective ion monitoring mode (GC–MS/SIM). Students utilized QuEChERS to analyze spiked and incurred pesticide residues in several types of plant foods and applied GC–MS/SIM for the simultaneous quantitation and identification of pesticides. Several chemistry, laboratory, and instrumental concepts were demonstrated such as sample preparation, aspects of method validation, and interpretation of chromatographic and mass spectrometric results. This experiment received favorable responses from the students because of the “real-world” applicability of the QuEChERS procedure, the use of GC–MS analysis, and concepts transferred from lecture to the laboratory.

中文翻译：

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QuEChERS和气相色谱-质谱法分析植物食品中的农药：本科生实验

向环境化学实验室课程的本科生授课QuEChERS（快速，简便，便宜，有效，坚固耐用和安全），这是一种农药制备实验室中常用的样品制备程序，涉及从新鲜产品样品中提取乙腈盐析，然后再进行乙腈盐析。结合使用吸附剂进行固相分散净化。清洗后的提取物被溶剂交换成甲苯，并通过毛细管气相色谱-质谱法以选择性离子监测模式（GC-MS / SIM）对农药进行分析。学生利用QuEChERS分析了几种植物性食物中加标的和引起的农药残留，并应用GC-MS / SIM进行农药的同时定量和鉴定。演示了几种化学，实验室和仪器概念，例如样品制备，方法验证的各个方面，以及色谱和质谱结果的解释。由于QuEChERS程序的“现实世界”适用性，GC-MS分析的使用以及从讲座到实验室的概念转移，该实验得到了学生的好评。