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Amycolatopsis alkalitolerans sp. nov., isolated from Gastrodia elata Blume.
The Journal of Antibiotics ( IF 2.1 ) Pub Date : 2019-08-21 , DOI: 10.1038/s41429-019-0222-8
Manik Prabhu Narsing Rao 1 , Yan-Qiong Li 2 , Hui Zhang 2 , Zhou-Yan Dong 1 , Awalagaway Dhulappa 3 , Min Xiao 1 , Wen-Jun Li 1
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A Gram-staining positive and nonmotile strain designated SYSUP0005T was isolated from tubers of Gastrodia elata Blume. The 16S rRNA gene sequence result showed that strain SYSUP0005T shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%). Growth occurs at 14–37 °C (optimum temperature, 28 °C), at pH 6–9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl. Strain SYSUP0005T had meso-diaminopimelic acid in its peptidoglycan. The whole cell sugars were galactose, ribose, and xylose. The predominant menaquinone was MK-9(H4) and minor menaquinones were MK-9(H2) and MK-9(H8). The polar lipids were diphosphatidylglycerol (DPG); phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phosphatidylinositol (PI), unidentified glycolipid (GL), and unidentified phospholipid (PL). The genomic DNA G + C content was 69.6 mol%. The major fatty acids were iso-C16:0, anteiso-C17:0, C16:0, iso-C14:0, C17:1 ω6c, C17:0, and Summed Feature 3 (C16:1 ω7c/C16:1 ω6c). On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp. nov. is proposed. The type strain is SYSUP0005T (=KCTC 49024T = CGMCC4.7463T).



中文翻译:

耐霉菌霉菌。十一月。,与天麻分离。

革兰氏染色指定SYSUP0005阳性和无动力应变Ť从的块茎中分离出天麻布卢姆。16S rRNA基因序列的结果表明,菌株SYSUP0005 Ť共享同的型菌株最高序列相似性拟无枝酸cappadoca(95.7%),拟无枝酸黄山松(95.4%),拟无枝酸pigmentata(95.4%),拟无枝酸ruanii(95.1%),和拟无枝酸Helveola(94.8%)。生长在14–37°C(最适温度,28°C),pH 6–9(最适pH 8)和最高6%(w / v)NaCl的存在下发生。菌株SYSUP0005 T具有介观-二氨基庚二酸在其肽聚糖中。全细胞糖是半乳糖,核糖和木糖。主要的甲萘醌为MK-9(H 4),次要的甲萘醌为MK-9(H 2)和MK-9(H 8)。极性脂质是二磷脂酰甘油(DPG);磷脂酰单甲基乙醇胺(PME),磷脂酰乙醇胺(PE),磷脂酰肌醇(PI),未鉴定的糖脂(GL)和未鉴定的磷脂(PL)。基因组DNA G + C含量为69.6 mol%。的主要脂肪酸为异-C 16:0,反异-C 17:0,C 16:0,异-C 14:0,C 17:1个 ω 6 Ç,C 17:0,和求和功能3(C16:1个 ω 7 ç / C 16:1个 ω 6 Ç)。上的表型,系统发育,化学分类的字符,和基因组比较的基础上,SYSUP0005 Ť表示属的一个新种拟无枝酸,为此,名称拟无枝酸alkalitolerans藻。十一月 被提议。类型应变为SYSUP0005 T(= KCTC 49024 T  = CGMCC4.7463 T)。

更新日期:2020-01-16
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