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In-situ silver nanoparticles formation as a tool for non-enzymatic glucose sensing: Study with an enzyme mimicking salt
Colloids and Surfaces A: Physicochemical and Engineering Aspects ( IF 3.990 ) Pub Date : 2019-07-29 , DOI: 10.1016/j.colsurfa.2019.123715
Vijay D. Yadav,R. Akhil Krishnan,Ratnesh Jain,Prajakta Dandekar

Enzyme mimicking materials have attracted significant interest due to their ability to replace expensive and unstable enzymes, in a range of applications. The present study is focused on an effective and economic metal salt that mimics enzymatic catalysts, in oxidising D-glucose, for glucose analysis. This enzyme mimicking salt, termed as ‘salzyme’, mimics the activity of D-glucose oxidase, by converting D-glucose to D-gluconic acid, along with spontaneous formation of silver nanoparticles (AgNPs) and hydrogen peroxide. The resulting AgNPs were characterized by TEM, for determining their size and morphology and UV spectroscopy for estimation of D-glucose. The detection of hydrogen peroxide produced during D-glucose oxidation was confirmed by dye tests. The results suggest the potential of this enzyme mimicking salt for further exploration in diagnostic and therapeutic applications.



中文翻译:

原位形成银纳米颗粒作为非酶葡萄糖感测的工具:用模拟盐的酶进行研究

由于在许多应用中,模仿酶的材料具有替代昂贵且不稳定的酶的能力,因此引起了人们的极大兴趣。本研究集中于一种有效且经济的金属盐,该金属盐在氧化D-葡萄糖时模拟酶催化剂,用于葡萄糖分析。这种酶模仿盐,称为“盐酶”,通过将D-葡萄糖转化为D-葡萄糖酸以及自发形成银纳米颗粒(AgNP)和过氧化氢来模仿D-葡萄糖氧化酶的活性。通过TEM表征所得的AgNP,以确定其大小和形态,并通过UV光谱法评估D-葡萄糖。通过染料测试证实了在D-葡萄糖氧化过程中产生的过氧化氢的检测。

更新日期:2019-07-29
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