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Encoded Microneedle Arrays for Detection of Skin Interstitial Fluid Biomarkers.
Advanced Materials ( IF 27.4 ) Pub Date : 2019-07-04 , DOI: 10.1002/adma.201902825
Xiaoxuan Zhang 1 , Guopu Chen 2 , Feika Bian 1 , Lijun Cai 1 , Yuanjin Zhao 1
Affiliation  

Skin interstitial fluid (ISF) is considered as an emerging source of biomarkers with physiological and medical significance. Microneedle arrays (MNs) provide a promising means for painless, noninvasive detection of these biomarkers. Here, novel MNs integrated with photonic crystal (PhC) barcodes are presented, and multiplex specific detection of ISF biomarkers is realized for the first time. The PhC barcodes-loaded flexible MNs are simply fabricated by replicating dynamic ferrofluid-cast micromoldings. When the prepared MNs are inserted into skin, they can enrich specific biomarkers to their probes-decorated PhC barcodes. Thus, by adding corresponding fluorescent probes to form sandwich immunocomplexes, the relative content of the biomarkers can be read out through the fluorescence intensity of the barcodes; meanwhile, the species of these biomarkers can be clearly distinguished by the reflection peaks of the PhC barcodes. Based on the encoded MNs, their sensitivity, flexibility, and versatility of capturing and detecting three inflammatory cytokines are demonstrated in a sepsis mice model. Compared with existing MNs for ISF detection, the encoded MNs not only possess equivalent detection effects with less post-processing and simplified procedures, but can also detect multiple biomarkers simultaneously, which makes them ideal in many clinical and biomedical detection areas.

中文翻译:

用于检测皮肤间质液生物标志物的编码微针阵列。

皮肤间质液(ISF)被认为是具有生理和医学意义的生物标志物的新兴来源。微针阵列(MNs)为无痛,无创地检测这些生物标记物提供了一种有希望的手段。在这里,提出了与光子晶体(PhC)条形码集成的新型MN,并且首次实现了ISF生物标记物的多重特异性检测。装有PhC条形码​​的柔性MN可以通过复制动态铁磁流体浇铸微成型件简单地制造出来。将准备好的MN插入皮肤后,它们可以在其探针修饰的PhC条码上丰富特定的生物标记。因此,通过添加相应的荧光探针形成夹心免疫复合物,可以通过条形码的荧光强度来读出生物标志物的相对含量。同时,这些生物标记的种类可以通过PhC条码的反射峰清楚地区分。基于编码的MN,在脓毒症小鼠模型中证明了它们捕获,检测三种炎性细胞因子的敏感性,灵活性和多功能性。与现有的用于ISF检测的MN相比,编码后的MN不仅具有同等的检测效果,而且后处理更少,流程更简单,而且可以同时检测多个生物标志物,因此使其在许多临床和生物医学检测领域均非常理想。
更新日期:2019-07-04
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