A novel closed bipolar electrode (cBPE) based click-light sensing strategy is prepared with integration of Au@MnO2 nanozyme for multi-mode visible detection of multiple targets, which have been widely applied in clinical tumor screening tests. cBPE benefiting from its easy requirement (one direct current power), portability, fast response and low background, has been an emerging device for construction of biosensors by integrating ultra-highly sensitive electrochemiluminescence (ECL) technique and shows great potential for point-of-care detection. However, existing cBPE-ECL methods are utilized at a high potential for ECL response, which greatly limits its sustainable and portable applications. Herein, a solid-state luminescent semiconductor, light emitting diode (LED) was first integrated with cBPE systems to provide significantly higher luminous efficiency in lower voltage and visualized fast readout in the sensing system. As target analytes (alpha-fetoprotein, prostate-specific antigen, and carcinoembryonic antigen) bind to the antibody-modified electrode surface, the nanozyme-labeled secondary antibodies (Ab2@Au@MnO₂) accelerate H2O2 decomposition, releasing more electrons. These electrons are transferred to the LED through the cBPE, increasing its luminous intensity, thus achieving simultaneous detection of multiple targets with low detection limits. Given the wide and urgent requirement of point-of-care devices, its potential applications in the construction of clinical sensing platform are expected.

中文翻译：

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多通道封闭式双极电极 LED 传感阵列，可同时灵敏可见地检测多种肿瘤标志物

构建了一种基于闭合双极电极 （cBPE） 的新型点击光传感策略，集成 Au@MnO2 纳米酶，用于多个靶点的多模式可见检测，已在临床肿瘤筛查试验中得到广泛应用。cBPE 受益于其简单要求（一个直流电源）、便携性、快速响应和低背景，通过集成超高灵敏度电化学发光 （ECL） 技术成为构建生物传感器的新兴设备，并在即时检测中显示出巨大的潜力。然而，现有的 cBPE-ECL 方法被用于 ECL 反应的可能性很高，这极大地限制了其可持续和可移植的应用。在此，固体发光半导体发光二极管 （LED） 首次与 cBPE 系统集成，以在较低电压下提供显着更高的发光效率，并在传感系统中实现可视化的快速读数。当靶标分析物（甲胎蛋白、前列腺特异性抗原和癌胚抗原）与抗体修饰的电极表面结合时，纳米酶标记的二抗 （Ab2@Au@MnO₂） 加速 H2O2 分解，释放更多电子。这些电子通过 cBPE 转移到 LED，增加其发光强度，从而实现对多个目标的同时检测，检测限低。鉴于床旁设备的广泛和迫切需求，其在临床传感平台建设中的潜在应用有望得到应用。