Biosynthesis of 12-aminododecanoic acid (ADDA) directly from biomass-derived sugars would enable a more sustainable process for manufacturing the engineering polymer Nylon 12. ADDA biosynthesis is currently hindered by the cytotoxicity of dodecanoic acid (DDA) to growing cells, and the accumulation of the overoxidized byproduct dodecanedioic acid (DDDA). In this study, these challenges were addressed by engineering an autoinducible system to better control in vivo DDA synthesis without impacting growth, and deleting aldehyde dehydrogenases and oxidases to reduce DDDA accumulation. As a result, a one-step fermentation process was established to synthesize ADDA from glucose and cellobiose. Finally, batch fermentation achieved 1035 mg/L ADDA and 5% yield, which is the highest titer and yield accomplished directly from sugar to date. This research contributes to the mechanistic understanding of microbial DDA, ADDA, and DDDA synthesis, as well as the goal of developing more sustainable processes for nylon production.

中文翻译：

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从生物质糖中生物合成 12-氨基十二酸


直接从生物质衍生的糖中生物合成 12-氨基十二烷酸 （ADDA） 将使制造工程聚合物尼龙 12 的工艺更加可持续。ADDA 生物合成目前受到十二烷酸 （DDA） 对生长细胞的细胞毒性以及过氧化副产物十二烷酸 （DDDA） 的积累的阻碍。在这项研究中，通过设计一种自诱导系统来更好地控制体内 DDA 合成而不影响生长，并删除醛脱氢酶和氧化酶以减少 DDDA 积累，从而解决了这些挑战。因此，建立了一步发酵工艺，从葡萄糖和纤维二糖合成 ADDA。最后，分批发酵实现了 1035 mg/L 的 ADDA 和 5% 的产量，这是迄今为止直接从糖中获得的最高滴度和产量。这项研究有助于对微生物 DDA、ADDA 和 DDDA 合成的机理理解，以及为尼龙生产开发更可持续的工艺的目标。