Gene expression profiling of tissue cells with spatial context is in high demand to reveal cell types, locations, and intercellular or molecular interactions for physiological and pathological studies. With rapid advances in barcoding chemistry and sequencing chemistry, spatially resolved transcriptome (SRT) techniques have emerged to quantify spatial gene expression in tissue samples by correlating transcripts with their spatial locations using diverse strategies. These techniques provide both physical tissue structure and molecular characteristics and are poised to revolutionize many fields, such as developmental biology, neuroscience, oncology, and histopathology. In this context, this Perspective focuses on next-generation sequencing-based SRT methods, particularly highlighting spatial barcoding chemistry. It delves into optically manipulated spatial indexing methods and DNA array-barcoded spatial indexing methods by exploring current advances, challenges, and future development directions in this nascent field.

中文翻译：

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基于下一代测序的空间转录组学：条形码化学的视角


迫切需要利用空间背景对组织细胞进行基因表达谱分析，以揭示细胞类型、位置以及细胞间或分子相互作用以进行生理和病理研究。随着条形码化学和测序化学的快速发展，空间分辨转录组（SRT）技术已经出现，通过使用不同的策略将转录本与其空间位置相关联来量化组织样本中的空间基因表达。这些技术提供了物理组织结构和分子特征，有望彻底改变许多领域，如发育生物学、神经科学、肿瘤学和组织病理学。在此背景下，本视角重点关注下一代基于测序的 SRT 方法，特别强调空间条形码化学。它通过探索这一新兴领域的当前进展、挑战和未来发展方向，深入研究光学操纵空间索引方法和 DNA 阵列条形码空间索引方法。