Deployment of nucleic acid amplification assays for diagnosing pathogens in point-of-care settings is a challenge due to lengthy preparatory steps. We present a molecular diagnostic platform that integrates a fabless plasmonic nano-surface into an autonomous microfluidic cartridge. The plasmonic ‘hot’ electron injection in confined space yields a ninefold kinetic acceleration of RNA/DNA amplification at single nucleotide resolution by one-step isothermal loop-mediated and rolling circle amplification reactions. Sequential flow actuation with nanoplasmonic accelerated microfluidic colorimetry and in conjugation with machine learning-assisted analysis (using our ‘QolorEX’ device) offers an automated diagnostic platform for multiplexed amplification. The versatility of QolorEX is demonstrated by detecting respiratory viruses: SARS-CoV-2 and its variants at the single nucleotide polymorphism level, H1N1 influenza A, and bacteria. For COVID-19 saliva samples, with an accuracy of 95% on par with quantitative polymerase chain reaction and a sample-to-answer time of 13 minutes, QolorEX is expected to advance the monitoring and rapid diagnosis of pathogens.

由于准备步骤冗长，在护理点部署核酸扩增检测来诊断病原体是一项挑战。我们提出了一个分子诊断平台，将无晶圆厂等离子体纳米表面集成到自主微流体盒中。通过一步等温环介导和滚环扩增反应，有限空间中的等离子体“热”电子注入使单核苷酸分辨率的 RNA/DNA 扩增产生九倍的动力学加速。顺序流驱动与纳米等离子体加速微流体比色法并与机器学习辅助分析（使用我们的“QolorEX”设备）相结合，为多重扩增提供了一个自动化诊断平台。QolorEX 的多功能性通过检测呼吸道病毒来证明：SARS-CoV-2 及其单核苷酸多态性水平的变体、H1N1 甲型流感和细菌。对于 COVID-19 唾液样本，QolorEX 的准确度为 95%，与定量聚合酶链反应相当，样本到答案时间为 13 分钟，预计将推进病原体的监测和快速诊断。