A Fe-loaded Bi₂O₂S nanosheet photoanode serving as photoelectric biomonitoring platform for the detection of prostate-specific antigen (PSA) using biologically inspired prussian nanoparticle (PB)-catalyzed biocatalytic precipitation strategy was developed. Primarily, the signal probe PB-mAb₂ obtained by electrostatic adsorption was immobilized on a microplate in the presence of target PSA, and 4-chloro-1-naphthol (4-CN) was oxidized to benzo-4-chloro-hexadienone (4-CD) with the assistance of exogenous hydrogen peroxide, which was generated by a large number of hydroxyl radicals catalyzed by PB. The generated 4-CD showed strongly low conductivity characteristics to burst the photocurrent of highly photoactive Fe–Bi₂O₂S photoanode. The split incubation reaction could be suitable for high volume and low-cost rapid detection. A dynamic response range of 0.1–100 ng mL⁻¹ with a limit of detection of 34.2 pg mL⁻¹ was achieved with the sensor based on a photoelectric sensing platform and a biomimetic catalytic precipitation reaction. Equally important, the sensor also showed good potential in the detection of real samples compared to commercially available ELISA kits. In conclusion, this work provides a fresh scheme for the development of sensitive biosensors through a bio-inspired catalytic strategy of versatility and a photoanode coupling with high photoelectric activity.

开发了一种负载 Fe 的 Bi ₂ O ₂ S 纳米片光阳极作为光电生物监测平台，使用受生物启发的普鲁士纳米颗粒 (PB) 催化的生物催化沉淀策略检测前列腺特异性抗原 (PSA)。首先，将静电吸附得到的信号探针PB-mAb ₂在目标PSA存在下固定在微孔板上，4-氯-1-萘酚（4-CN）被氧化成苯并-4-氯-己二烯酮（4 -CD)在外源性过氧化氢的辅助下，由PB催化产生大量羟基自由基。生成的 4-CD 表现出极低的电导率特性，可爆发高光活性 Fe–Bi ₂ O _{2的光电流}S光电阳极。分流孵育反应适用于高通量、低成本的快速检测。基于光电传感平台和仿生催化沉淀反应的传感器实现了0.1–100 ng mL ^{-1的动态响应范围和 34.2 pg mL -1}的检测限。同样重要的是，与市售的 ELISA 试剂盒相比，该传感器在检测真实样品方面也显示出良好的潜力。总之，这项工作通过多功能的仿生催化策略和具有高光电活性的光电阳极耦合，为灵敏生物传感器的开发提供了一种新的方案。