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Cytochrome P450 2D6 biosensor for perphenazine based on multi-walled carbon nanotube/ionic liquid and tetrathiafulvalene-tetracyanoquinodimethane salt/ionic liquid gels
Electrochimica Acta ( IF 5.5 ) Pub Date : 2022-12-01 , DOI: 10.1016/j.electacta.2022.141656
Ting Shu , Junyi Wang , Xiatong Li , Xiaobo Wang , Shi Wang

A cytochrome P450 2D6 (CYP2D6) biosensor was developed with the incorporation of multi-walled carbon nanotube/ionic liquid and tetrathiafulvalene-tetracyanoquinodimethane salt/ionic liquid (MWNT/IL-[TTF–TCNQ]/IL) gels. The MWNT/IL-[TTF–TCNQ]/IL gels were characterized using Fourier-transform infrared spectroscopy, ultraviolet–visible spectroscopy, and scanning electron microscopy. Utilizing the excellent stability of the MWNT/IL gel and the unique structure of the [TTF–TCNQ]/IL gel, CYP2D6 was immobilized on the screen-printed electrode with high bioactivity. Moreover, the CYP2D6 catalytic effect was highly promoted upon application of MWNT/IL-[TTF–TCNQ]/IL gels as the mediating system. The proposed MWNT/IL-[TTF–TCNQ]/IL gel-based CYP2D6 biosensor was used to detect perphenazine via voltammetry and amperometry. The calibration of perphenazine ranged from 6 × 10−8 to 1.6 × 10−5 M with a detection limit of 2.05 × 10−8 M. It is expected that the MWNT/IL-[TTF–TCNQ]/IL gel-based CYP2D6 biosensor opens new avenues for promising advances in the analysis of drugs.



中文翻译:

基于多壁碳纳米管/离子液体和四硫富瓦烯-四氰基喹啉二甲烷盐/离子液体凝胶的细胞色素P450 2D6奋乃静生物传感器

开发了一种细胞色素 P450 2D6 (CYP2D6) 生物传感器,结合了多壁碳纳米管/离子液体和四硫富瓦烯-四氰基喹啉二甲烷盐/离子液体 (MWNT/IL-[TTF–TCNQ]/IL) 凝胶。MWNT/IL-[TTF–TCNQ]/IL 凝胶使用傅立叶变换红外光谱、紫外-可见光谱和扫描电子显微镜进行了表征。利用 MWNT/IL 凝胶优异的稳定性和 [TTF–TCNQ]/IL 凝胶的独特结构,将 CYP2D6 固定在具有高生物活性的丝网印刷电极上。此外,在应用 MWNT/IL-[TTF–TCNQ]/IL 凝胶作为中介系统后,CYP2D6 的催化作用得到了极大的促进。所提出的基于 MWNT/IL-[TTF–TCNQ]/IL 凝胶的 CYP2D6 生物传感器用于通过伏安法和安培法检测奋乃静。−8至 1.6 × 10 −5  M,检测限为 2.05 × 10 −8  M。预计基于 MWNT/IL-[TTF–TCNQ]/IL 凝胶的 CYP2D6 生物传感器开辟了新途径,有望在药物分析。

更新日期:2022-12-01
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