Lipophilization is a promising way to improve the bioavailability of flavonoids. However, the traditional enzymatic esterification methods are time-consuming, and present low yields and purity. Herein, a novel membrane-based lipophilization technology—bioinspired lipase immobilized membranes (BLIMs), including CAL-B@PES, CAL-B@PDA/PES and GA/CAL-B@PDA/PES— were fabricated to improve the antioxidant flavanone glycoside hesperidin lipophilization. Via reverse filtration, PDA coating and GA crosslinking, Candida antarctica lipase B (CAL-B) was stably immobilized on membrane to fabricate BLIMs. Among the three BLIMs, GA/CAL-B@PDA/PES had the greatest enzyme activity and enzyme loading, the strongest tolerance of changes in external environmental conditions (temperatures, pH, heating time, storage time and numbers of cycles) and the highest hesperidin esterification efficiency. Moreover, the optimal operating condition for GA/CAL-B@PDA/PES fabrication was the CAL-B concentration of 0.36 mg/mL, operation pressure of 2 bar, GA concentration of 5% and crosslinking time of 1 h. Afterwards, the hesperidin esterification process did not affect the micromorphology of BLIM, but clearly improved the BLIM permeability and esterified product efficiency. The present study reveals the fabrication mechanism of BLIMs and offers insights into the optimizing strategy that governs the membrane-based lipophilization technology process.

中文翻译：

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用于提高橙皮苷亲脂化的仿生脂肪酶固定膜

亲脂化是提高类黄酮生物利用度的一种有前途的方法。然而，传统的酶酯化方法耗时长，收率低，纯度低。在此，制备了一种基于膜的新型亲脂化技术——仿生脂肪酶固定化膜（BLIMs），包括 CAL-B@PES、CAL-B@PDA/PES 和 GA/CAL-B@PDA/PES，以提高抗氧化黄烷酮的含量。糖苷橙皮苷亲脂化。通过反向过滤、PDA 涂层和 GA 交联，南极念珠菌脂肪酶 B (CAL-B) 稳定地固定在膜上以制造 BLIM。在三种 BLIM 中，GA/CAL-B@PDA/PES 的酶活性和酶载量最大，对外部环境条件（温度、pH、加热时间、储存时间和循环次数）变化的耐受性最强，最高橙皮苷酯化效率。此外，GA/CAL-B@PDA/PES制备的最佳操作条件是CAL-B浓度为0.36 mg/mL，操作压力为2 bar，GA浓度为5%，交联时间为1 h。之后，橙皮苷酯化过程对 BLIM 的微观形貌没有影响，但明显提高了 BLIM 的渗透性和酯化产物的效率。