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Genetic diversity of Lansium parasiticum (Osbeck) K. C. Sahni & Bennet accessions based on vegetative morphological characters and simple sequence repeat markers
Genetic Resources and Crop Evolution ( IF 1.6 ) Pub Date : 2022-01-08 , DOI: 10.1007/s10722-021-01336-9
Darda Efendi 1 , Willy Bayuardi Suwarno 1 , Deden Derajat Matra 1 , Hevia Purnama Sari 2
Affiliation  

Morphological characters and SSR (Simple Sequence Repeat) molecular marker information were combined to study the genetic diversity of Lansium domesticum Corrêa. The research aimed to study the genetic diversity of accessions of L. parasiticum (duku, langsat, kokosan) accessions based on morphological characters and SSR markers. The field research was conducted at the Bogor Botanical Gardens, Mekarsari Fruit Park, and Condet Fruit Reserve. Morphological characterization was carried out by direct observation. SSR was characterized by using the Type-it Microsatellite PCR kit protocol. Data were analyzed using Cervus 3.07, POPGENE 3.2, and PBSTAT-CL 2.1. Accessions formed four groups at a cophenetic distance of 0.45. Duku condet is in group one. Group two was identified as kokosan-langsat, while the other groups’ names could not be identified. Analysis based on SSR markers yielded two main groups at a cophenetic distance of 0.35. Almost group one consisted of accessions with local names duku and langsat, while group two consisted of accessions with local names of kokosan. Primers P1, P4, P5, and P8 are the best primers in distinguishing these two groups. The dendrogram resulted from the combined data analysis formed two groups at a cophenetic distance of 0.35. The observed genetic variability among accessions was high based on morphological and molecular characters. The combination of morphological and SSR markers can distinguish accession with the vernacular name langsat and duku but cannot distinguish accessions with the vernacular name duku since langsat and kokosan are separate groups while duku is in both groups.



中文翻译:

基于营养形态特征和简单序列重复标记的 Lansium parasiticum (Osbeck) KC Sahni & Bennet 种质的遗传多样性

结合形态学特征和SSR(Simple Sequence Repeat)分子标记信息,研究Lansium domesticum Corrêa的遗传多样性。本研究旨在研究寄生乳杆菌种质的遗传多样性(duku, langsat, kokosan) 基于形态特征和 SSR 标记的种质。实地研究是在茂物植物园、梅卡萨里水果公园和康代水果保护区进行的。通过直接观察进行形态表征。SSR 通过使用 Type-it 微卫星 PCR 试剂盒方案进行表征。使用 Cervus 3.07、POPGENE 3.2 和 PBSTAT-CL 2.1 分析数据。种质以 0.45 的共同距离形成四个组。Duku condet 在第一组。第二组被确定为 kokosan-langsat,而其他组的名称无法确定。基于 SSR 标记的分析在 0.35 的共同距离处产生了两个主要组。几乎第一组由本地名称为 duku 和 langsat 的种质组成,而第二组由本地名称为 kokosan 的种质组成。引物 P1、P4、P5 和 P8 是区分这两组的最佳引物。由组合数据分析得出的树状图在 0.35 的共同距离处形成了两组。基于形态和分子特征,观察到的种质之间的遗传变异性很高。形态学和 SSR 标记的组合可以区分白话名 langsat 和 duku 的种质,但不能区分白话名 duku 的种质,因为 langsat 和 kokosan 是不同的组,而 duku 在两个组中。

更新日期:2022-01-09
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