Bacterial resistance is a natural process carried out by bacteria, which has been considered a public health problem in recent decades. This process can be triggered through the efflux mechanism, which has been extensively studied, mainly related to the use of natural products to inhibit this mechanism. To carry out the present study, the minimum inhibitory concentration (MIC) tests of the compound limonene were performed, through the microdilution methodology in sterile 96-well plates. Tests were also carried out with the association of the compound with ethidium bromide and ciprofloxacin, in addition to the ethidium bromide fluorimetry, and later the molecular docking. From the tests performed, it was possible to observe that the compound limonene presented significant results when associated with ethidium bromide and the antibiotic used. Through the fluorescence emission, it was observed that when associated with the compound limonene, a greater ethidium bromide fluorescence was emitted. Finally, when analyzing the in silico study, it demonstrated that limonene can efficiently fit into the MepA structure. In this way, it is possible to show that limonene can contribute to cases of bacterial resistance through an efflux pump, so that it is necessary to carry out more studies to prove its effects against bacteria carrying an efflux pump and assess the toxicity of the compound.

细菌耐药性是细菌进行的自然过程，近几十年来一直被认为是一个公共卫生问题。这一过程可以通过外排机制触发，该机制已被广泛研究，主要与使用天然产物抑制这一机制有关。为了进行本研究，通过无菌 96 孔板中的微量稀释方法，对化合物柠檬烯进行了最低抑制浓度 (MIC) 测试。除了溴化乙锭荧光测定法和后来的分子对接外，还对该化合物与溴化乙锭和环丙沙星的关联进行了测试。从所进行的测试中可以观察到，当与溴化乙锭和所使用的抗生素相关时，化合物柠檬烯呈现出显着的结果。通过荧光发射，观察到当与化合物柠檬烯结合时，发出更大的溴化乙锭荧光。最后，在分析计算机研究时，证明柠檬烯可以有效地融入 MepA 结构。通过这种方式，有可能表明柠檬烯可以通过外排泵导致细菌耐药的情况，因此有必要进行更多的研究以证明其对携带外排泵的细菌的作用并评估该化合物的毒性.