Bisulfite (HSO₃⁻) has been widely used in food and industry, which has brought convenience to human life, but also seriously endangered human health. In this work, the probe PBI was designed and synthesized to detect bisulfite (HSO₃⁻) through nucleophilic addition reaction. The probe PBI showed a selective reaction to HSO₃⁻ and can quantitatively detect HSO₃⁻. At the same time, the color of the probe PBI changed significantly, which provided a simple method for the naked eye to identify HSO₃⁻. Finally, it was successfully applied to the fluorescence imaging of HSO₃⁻ in living cells.

Graphical abstract

中文翻译：

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使用 NIR 荧光染料对 HSO3− 进行比色和比例荧光检测

亚硫酸氢盐(HSO ₃ ⁻ )广泛应用于食品和工业，在给人类生活带来方便的同时，也严重危害着人类的健康。在这项工作中，设计并合成了探针 PBI ，通过亲核加成反应检测亚硫酸氢盐 (HSO ₃ ^{- )。}探针PBI对HSO ₃ ^-表现出选择性反应，可以定量检测HSO ₃ ^-。同时，探针PBI的颜色发生明显变化，为肉眼识别HSO ₃ ^-提供了一种简便的方法。最后，它成功地应用于活细胞中HSO ₃ ^-的荧光成像。

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