The extensive abuse of chemical synthetic additives has raised increased attention to food safety. As substitutes, probiotics play an important role in human health as they balance the intestinal microbes in host. This study was aimed to isolate and evaluate the potential probiotic activities of lactic acid bacteria (LAB) from a local pickled leaf mustard (PLM) from Wuwei city in Anhui province through in vitro experiments. A total of 17 LAB strains were obtained as probiotics. All the isolates were sensitive to chloramphenicol, tetracycline, erythromycin, and doxycycline but exhibited resistance to antibiotics (e.g., streptomycin, kanamycin, gentamicin, and vancomycin). Out of the 17 strains, 9 were sensitive to most of the antibiotics and had no cytotoxic activity on human colorectal adenocarcinoma cell line (HT-29) cells. The isolated AWP4 exhibited antibacterial activity against four indicator pathogen strains (ATCC8099: Escherichia coli , ATCC6538: Staphylococcus aureus , ATCC9120: Salmonella enteric , and BNCC192105: Shigella sonnei ). Based on the phylogenetic analysis of the 16S rRNA gene, AWP4 belonged to Lactiplantibacillus plantarum . This study indicated that the Wuwei local PLM could be a potential resource to isolate beneficial LAB as probiotics. The data provide theoretical guidance for further animal experiments to estimate the probiotic effect and safety of Lpb. plantarum AWP4 in vivo .

中文翻译：

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安徽武威地方榨菜中分离的具有益生菌活性的乳酸菌作为化学合成添加剂的替代品的体外评价

化学合成添加剂的广泛滥用引起了人们对食品安全的日益关注。作为替代品，益生菌在人体健康中发挥着重要作用，因为它们可以平衡宿主的肠道微生物。本研究旨在通过体外实验从安徽省武威市当地腌制芥菜（PLM）中分离和评估乳酸菌（LAB）的潜在益生菌活性。共获得 17 株 LAB 菌株作为益生菌。所有分离株均对氯霉素、四环素、红霉素和强力霉素敏感，但对抗生素（如链霉素、卡那霉素、庆大霉素和万古霉素）表现出耐药性。在这 17 个菌株中，9 个对大多数抗生素敏感，并且对人结肠直肠腺癌细胞系 (HT-29) 细胞没有细胞毒活性。分离的 AWP4 对四种指示性病原体菌株（ATCC8099：大肠杆菌、ATCC6538：金黄色葡萄球菌、ATCC9120：肠道沙门氏菌和 BNCC192105：志贺氏菌）表现出抗菌活性。根据16S rRNA基因的系统发育分析，AWP4属于植物乳杆菌。该研究表明，武威本地PLM可能是将有益乳酸菌分离为益生菌的潜在资源。该数据为进一步的动物实验以评估 Lpb 的益生菌作用和安全性提供了理论指导。体内植物 AWP4。AWP4属于植物乳杆菌。该研究表明，武威本地PLM可能是将有益乳酸菌分离为益生菌的潜在资源。该数据为进一步的动物实验以评估 Lpb 的益生菌作用和安全性提供了理论指导。体内植物AWP4。AWP4属于植物乳杆菌。该研究表明，武威本地PLM可能是将有益乳酸菌分离为益生菌的潜在资源。该数据为进一步的动物实验以评估 Lpb 的益生菌作用和安全性提供了理论指导。体内植物 AWP4。